... distalfemora were harvested, cleaned of soft tissue, and fixedin 70% ethanol.The femur bone was trimmed above and below the ends of the implant, cleaned of soft-tissue, and fixe d in 70%alcohol. The ... implant and thebone and soft-tissue pixels adjacent to the edge of theimplants were counted.Osseointegration was de fined as bone-to-imp lant con-tact and calculated as the ratio of the number of ... and 255), bone pixels (grayscale value between 80 and 200), and soft-tissue pixels (grayscale value between0 and 80). An edge detection algorithm was used todetect pixels at the perimeter of...