... concentrations of NaCl: 25 mM (lanes 2 and 8), 50 mM (lanes 3 and 9), 100 mM (lanes 4 and 10), 200 mM (lanes 5 and 11), 500 mM (lanes 6 and 12), and 1000 mM (lanes 7 and 13). Lanes 2–7 show the dissociation ... were washed twice with serum-free medium, inoculated with the DNA- Lipo-fectamine mixture, and incubated at 37°C for 6 hours.The DNA solution was replaced with complete medium and the cells wee ... expanding, carry mutations inTat R57S and G63Q within and close to the basic domain, and yet exhibit increased transcriptional activity [40]. Onthe other hand, the phosphorylation of serines and...