báo cáo hóa học:" Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" ppt

... Helsinki, FinlandEmail: Angelina Plyusnina - anguelina.pljusnina@helsinki.fi; Alexander Plyusnin* - alexander.plyusnin@helsinki.fi* Corresponding author Abstract Tula hantavirus carrying recombinant ... parental virus: analysis of consecutive passages in a cell cultureAngelina Plyusnina and Alexander Plyusnin*Address: Haartman Institute, Department of Virology, University of Helsinki POB 21, FIN-00014, ... Gilljam M, KanervaM, Manni T, Pejcoch M, Niemimaa J, Kaikusalo A, Henttonen H,Vaheri A, Plyusnin A: Isolation and characterization of Tula virus: a distinct serotype in genus Hantavirus, family...

Báo cáo sinh học: " Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" pot

... in this paper show that the recTULVpresents no real match to the original cell adapted variantand that the lower fitness of the recombinant virus can notbe increased by pre-passaging in cell ... weaker due to the fact that two non-canonical G:Ubase pairs presented in the plus-sense RNA occur as non-pairing C /A bases in the minus-sense RNA. Interestingly, in Puumala hantavirus, a hairpin-like ... Pejcoch M, Niemimaa J, Kaikusalo A, Henttonen H,Vaheri A, Plyusnin A: Isolation and characterization of Tula virus: a distinct serotype in genus Hantavirus, family Bunya-viridae. J Gen Virol...

báo cáo hóa học: " Work-related stress and bullying: gender differences and forensic medicine issues in the diagnostic procedure" pdf

... Public Health and Neuroscience, University of Pavia, Pavia,ItalyFull list of author information is available at the end of the articleTonini et al . Journal of Occupational Medicine and Toxicology ... and interpreted the data. FM collected,analyzed and interpreted the data. CF collected, analyzed and interpreted the data, performed the statistical analysis. FS participated in the design of the ... correctly. To calculate the scores, a computer program and a manual scoring are available. The interpretation of results requires a high level of psy-chometric, clinical, professional and characteriologicalcompetence.The...

Báo cáo hóa học: " Research Article Robust Object Categorization and Segmentation Motivated by Visual Contexts in the Human Visual System" docx

... F) is the relative frequency of codebook F in object instance l.We have to minimize intraclass variations. As mentioned,one of the main causes of large intraclass variation is surface markings, ... comparing visual appearance. In Figure 10, wheels in the car codebook and in airplanecodebook have a similar appearance. At the same time,each category also has a contextually related backgroundcodebook. ... capability of intraclass variation is higher. At the same time, the discrimination power is reduced due to the weak spatial relation. Since the conventional principlecomponent analysis (PCA) can represent...

báo cáo sinh học:" What impact do Global Health Initiatives have on human resources for antiretroviral treatment roll-out? A qualitative policy analysis of implementation processes in Zambia" pptx

... but seconding them, as in the case of additional provincial clinical care specialists, additionalcapacity remains external and limited to the period of GHIfunding available. The more Zambia's ... of their clinic, imposing a further strain on the day -to- day running of the ART programme.Mentoring and the secondment of GHI staff to the public sector In addition to training, the PEPFAR ... directorates in each of Zambia's nine provinces.While each of these interventions aims to alleviate the human resource shortage in relation to ART, examiningtheir impact at district and...

Báo cáo hóa học: " Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands" pdf

... icosahedron. The P domain is divided into sub-domains P1 and P2 that participate in dimeric contactsthat increase the stability of the capsid. The P2 domain is an insertion in the P1 domain and ... 5'- GGG GAT ATC CAC CAT GGA GAC AGA CAC ACT CCT GCT AT -3'VK2-F 5'- GGG GAT ATC CAC CAT GGA TTT TCA AGT GCA GAT TTT CAG -3'VK3-F 5'- GGG GAT ATC CAC CAT GGA GWC ACA ... hemagglutination. In this study, we engineered the antigen binding domains of mAb 54.6 into a single chain variablefragment (scFv) and tested whether these scFv could function as cell binding inhibitors,...

báo cáo hóa học:" Recombinant HPV16 E7 assembled into particles induces an immune response and specific tumour protection administered without adjuvant in an animal model" doc

... madecontribution in data analysis; CG made contribution to the analysis andinterpretation of the data, in critical revision of the manuscript and in acquisition of funding. PDB conceived and designed ... the study, analysed andinterpreted the data and drafted the manuscript. All authors read andapproved the final manuscript.Authors’ informationLP’s present address: Istituto Nazionale Malattie ... Elena 299, 00161 Rome, Italy.Authors’ contributionsLP carried out the biochemical and immunological assays, madecontribution to the analysis and interpretation of the data and helped to draft...

báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx

... N-terminal His-tag to allow a functional expression of the 2,5-DKCMO in E. coliand easy protein purification by affinity chromatography. The N-terminal tag was favored compared to the C-terminal tag, ... (DBU, Osnabrück,Germany, Grant No. AZ13234) for financial support and Christin Peters andIna Menyes for assistance in the laboratory.Competing interests The authors declare that they have no ... 1,2-monoox-ygenase considerably and could thus gene rate valuablecatalysts for organic synthesis providing access to indus-trial valuable precursors for e.g. azadirachtin.Regarding the requirement for cofactor...

báo cáo hóa học:" Recombinant bromelain production in Escherichia coli: Process optimization in shake flask culture by Response Surface Methodology" pptx

... respectively. The analysis of variance (ANOVA) tested using Fisher’s statistical analysis, was used to verify the adequacy of the model. The results are shown in Table 3. The model F-value of 99.32 ... activity of recombinant bromelain. An increase in recombinant bromelain production was seen by increasing the L-arabinose concentrations from 0.1−0.2 % (w/v). The maximum recombinant protein production ... information on the relationship between the response and experimental levels of each variable. They also explain the type of interaction existing between the test variables and help to obtain the...

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