... JournalOpen AccessShort report A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoterLaurent Mailly1,2, Charlotte Boulade-Ladame1, Georges Orfanoudakis1 ... shuttle plasmid containing a promoter for the expression of the transgene. After selection of recombinants, the plasmid DNA of each clone has to betransferred into an other bacterial strain (i.e. ... recombinant adenoviral vectors. HumGene Ther 1999, 10(12):2013-2017.7. Mizuguchi H, Kay MA, Hayakawa T: In vitro ligation-based cloning of foreign DNAs into the E3 and E1 deletion regions for...