... therefore discarded. Mass spectrometryconfirmed the two forms were distiguished by Cys95AMY1glutathionylation in the lower pI form [38,50–52]. Thearomatic replacements Met53Trp/Tyr had no visible ... to C. Vincentsen for expert technicalassistance, L. H. Sørensen and the late B. Corneliussen for amino acidanalysis, and M B. Rask and the late J. Sage for DNA sequencing.This work was supported ... Perkin-Elmer) and the plasmid was used for P. pastoris transfor-mation by electroporation [50] upon linearization at theBstXI site. Screening was performed for Zeocin transform-ants on YPDS plates (1%...