... initially for SSCexpansion, containing LIF, putrescine, EGF, GDNF, bFGF,insulin and transferrin. The proliferating cells that resultedwere described as forming compact clusters and havingblurred ... zonapellucida.AcknowledgementsThe author is much indebted to Robert M. Moor for inspiring this article, and for his unstinting support and expert advice; and to Roger G. Gosden and Rus Hoelzel for their critical appraisal ... characteristics. For example,nestin [60] and Gata-4 [69] are markers for OSE stemcells. FoxA2 is known to be expressed in uterine glands[71], and expression in this culture system may there-fore be indicative...