... E D I C I N ETM Bone Research Protocols Edited byMiep H. Helfrich, PhDStuart H. Ralston, MD Bone Research Protocols Edited byMiep H. Helfrich, PhDStuart H. Ralston, MD Bone Cells from Calvariae ... receptors 1-4. Bone 27,185–195.01/Gall/1-18/F1 2/26/03, 10:44 AM18 Bone Cells from Calvariae and Long Bones 1919From: Methods in Molecular Medicine, Vol. 80: Bone Research Protocols Edited ... Calvariaeand Long BonesAstrid Bakker and Jenneke Klein-Nulend1. IntroductionWhen conducting in vitro research on bone, a choice has to be made betweenusing bone organ or bone cell cultures....