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Báo cáo Y học: Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin Characterization of different PAI-1 mutants pdf

Báo cáo Y học: Interaction between p21-activated protein kinase and Rac during differentiation of HL-60 human promyelocytic leukemia cell induced by all-trans-retinoic acid pdf

Báo cáo Y học: Interaction between p21-activated protein kinase and Rac during differentiation of HL-60 human promyelocytic leukemia cell induced by all-trans-retinoic acid pdf

... 1stoichiometry and with a Kdvalue of 6.7 nM.Keywords:Rac;PAK;HL-60;GTPase;MAPkinase.A phagocytic superoxide-generating system is expressedupon induced differentiation of HL-60 human promyelo-cytic ... previously [27].RESULTSInduction of gp91phoxand superoxide generatingactivity of HL-60 CellsUtilizing the induced differentiation of HL-60 promyelo-cytic leukemia cells as a model of myeloid ... of mature cell types, all stages from promyel-ocytes to polymorphonuclear leukocytes are present inboth. HL-60 cells induced with ATRA for 5 days showed40–60% of the NADPH oxidase activity...
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Báo cáo y học: "Grb2-associated binder 1 polymorphism was associated with the risk of Helicobactor pylori infection and gastric atrophy"

... Committee of the Nagoya University Graduate School of Medicine. Tests for Helicobacer pylori (H. pylori) antibody and pepsinogens Anti-H. pylori IgG antibody tests, high-molecular- weight campylobacter-associated-protein ... the genotype frequency and odds ratio (OR) of H. pylori seropositivity in healthy controls. The seropositivity rate for those with A/A was lowest. The decreased OR of A/A for H. pylori seropositivity ... significantly higher than the seronegative healthy controls in which atrophy was present in only 10% of subjects (p<0.001). Table 1 Characteristics of the study subjects by source of recruitment....
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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx

Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx

... expressed enzymes had nolyase activity towards PROG or 17-OHPROG.Understanding of the complexity of hydroxylase andlyase activity of CYP17 by way of primary sequencealignments of CYP17 from different ... diethylpyrocarbonate; DHEA, dehydro-epiandrosterone; DHEA-S, dehydroepiandrosterone-sulphate; DOC,deoxycorticosterone; CYP17, cytochrome P450 17a-hydroxylase;CYP21, cytochrome P450 21-hydroxylase; ... 17a-hydroxylase (CYP17) cata-lyses not only the 17a-hydroxlation of pregnenolone andprogesterone and the C17,20-side chain cleavage (lyase) of 17a-hydroxypregnenolone, necessary for the biosynthesis...
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Báo cáo Y học: Agmatine oxidation by copper amine oxidase Biosynthesis and biochemical characterization of N-amidino-2-hydroxypyrrolidine pdf

Báo cáo Y học: Agmatine oxidation by copper amine oxidase Biosynthesis and biochemical characterization of N-amidino-2-hydroxypyrrolidine pdf

... Values of kcatand K0mare independent of the enzyme assay.Biosynthesis of N-amidino-2-hydroxypyrrolidineN-Amidino-2-hydroxypyrrolidine was synthesized as fol-lows. Twenty micrograms of P. ... Federico11Department of Biology, University ÔRoma TreÕ, Rome, Italy;2Department of Structural and Functional Biology,University of Insubria, Varese, ItalyThe p roduct of agmatine oxidation catalyzed by Pisumsativum ... and of the trypsin catalyzed hydrolysis of N-a-benzoyl-L-arginine p-nitroanilide (at pH 6.8 and21.0 °C) by N-amidino-2-hydroxypyrrolidine were deter-mined from the linear dependence of the...
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Báo cáo Y học: Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin Characterization of different PAI-1 mutants pdf

Báo cáo Y học: Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin Characterization of different PAI-1 mutants pdf

... Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin Characterization of different PAI-1 mutants Nuria Arroyo De Prada1,*, Florian Schroeck1,*, ... andexerted an inhibitory activity against HMW-uPA of 90%(equivalent to 90 000 Uámg)1) after the two-step af®nitychromatography. Interaction of (mutant) PAI-1 with VnAll inhibitory active mutants ... 1MNaCl.The generated PAI-1 var iants did also not display anyinhibitory activity after puri®cation under native conditions.Again, the inhibitory activity of at least some of the PAI-1 mutants could...
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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf

Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf

... Interest-ingly, the specific inhibitory activity of G56A/Q334S wasslightly decreased by vitronectin. Latency transition of PAI-1 wild-type and PAI-1 variants inthe absence and the presence of vitronectin To ... andrefolded by dialysis against NaCl/Pi.Assays for measuring specific inhibitory activity of PAI-1 The specific inhibitory activity of the reactivated PAI-1 variants was measured by titration ... to PAI-1 wild-type (Table 3). Analysis of Table 2. Stability of specific inhibitory activity of PAI-1 variants at 37 8C. Means, SDs, and numbers of experiments are indicated. *, Significantlydifferent...
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Báo cáo y học: "Electronic patient record use during ward rounds: a qualitative study of interaction between medical staff"

... access to informa-tion in different ways, the change of record is likely to affect interaction. We therefore chose to compare how interaction was achieved with each type of record. The ward-round ... months after deployment of the system, andthe effects of the introduction of the EPR on group interaction were discussed. Patient privacy was ensured at all times byavoiding capture of images that ... interacting with each other using theelectronic record compared with the paper one. The physicalsetup of the technology may impede the consultant's ability tolead the ward round and may prevent...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

... iscrepancy could lie in the fact thattype II collagen is more glycosylated than type I collagen. Itseems to us probable that glycosylation of hydroxylysinewill block the binding. However, the glycosylation ... anhydride,indicating that the side-effects of the treatment with aceticanhydride described above are not responsible for the loss of binding. Methylation of Lys/Hyl residues by treatment with HCHO/NaBH3CN ... CastellaniÕ,University of Pavia, Italy). All other reagents were of analytical grade.Preparation and analysis of decorin from tendonDecorin was purified as described p reviously [31,32]. Brie y, proteoglycans...
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Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot

Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot

... lectivity of this process has been demonstrated unambiguously by thedifferential clearing of distinct proteins from the haemol-ymph [3–6]. Transport of h examerins t hrough f at body cellmembranes ... and in situ hybridization of Calliphora fat body. Longitudin al cryosections (10 lm) of 7-day-old larvae (A–C) were stained with a Cy2-conjugated goat anti-rabbit IgG after incubation with rabbit ... isrestricted to the cytoplasm. (C) AFP immunostaining can also be found in the cytoplasm of haemocytes. (D) In situ hybridization of longitudinalcryosections with a digoxygenin-labeled antisense...
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Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf

Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf

... Drakenberg21Department of Clinical Chemistry, University Hospital Malmo¨, Lund University, Malmo¨, Sweden;2Department of BiophysicalChemistry, Lund University, Lund, SwedenThe interaction of blood ... vicinity of the membrane.We found that the isolated factor X Gla domain exhibitslow affinity binding to PtdSer-containing membranes with aKd of 4.6 lM. This agrees well with the value of 2.4 ... that mutation of Ca2+ligatingamino acids in the N-terminal part of the first EGF-likedomain of factor X influences the amidolytic activity of theintact protein [36]. However, direct interactions...
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