Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... Sampieri, F.(1991) An anti-insect toxin puriﬁed from the scorpion Androctonusaustralis Hector also acts on the alpha- and beta-sites of the mammalian sodium channel: sequence and circular dichroismstudy. ... mammals and can acts on the a- andb-sites of the mammaliansodium channel [44]. Therefore, whether Tyr31 is relatedTable 2. The amounts of aconitine required to induce arrhythmia inuntreated rats ... Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin Fang Peng1, Xian-Chun Zeng1, Xiao-Hua He1, Jun Pu2,...

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... MW.Custom-made software and hardware were used for acquisition and analysis of data. Leakage and linear capacity currents weresubtracted by using P/4 protocols. Data were sampled onceevery 5 ls and ... shown are the mean (j) and standard deviation (bars) for each cRNA a: b ratio, and the sample size percRNA combination is shown in parenthesis. Data are from ﬁve diﬀerent batches of oocytes, each ... b1Btranscript is abundant in human brain and skeletal muscle (Fig. 2A) , and present at a very low levelFig. 2. Northern blot analysis of the gene expression of human b1B (A, B) and b1(C,D)subunits,...

Báo cáo khoa học: Identiﬁcation and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

... inﬂammation [27] and oncogenictransformation [28,29].BLASTanalysis of the mouse and human genomedatabases allowed us to identify an unknown sialyltrans-ferase gene encoding a second Galb1–4GlcNAc ... analysed by radio-imaging.Multiple tissue expression array and northern analysis An EcoRI–BamHI 1.6 kb fragment of the human ST6GalII cDNA and a 1.8 kb human b-actin cDNA (Clontech)used as a positive ... occipital and parietal lobe and salivary gland.Almost no expression was observed in fetal lung and heart,uterus, bladder, kidney, duodenum, trachea, Burkitt’s lym-phoma, and colorectal adenocarcinoma....

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... technical assistance and helpful discussion and toDr Gabriella Fiorentino, Dr Pietro Amodeo and DrFrancesca Maria Pisani for critical reading of thismanuscript. This work was grant-aided by the ... best of our knowledge, Rep245 typiﬁes the shortest functional domain from a crenarchaeal plasmid endowed withDNA and RNA synthesis and terminal transferase activity.AbbreviationsAEP, archaeo-eukaryotic ... [32P]dATP[aP] as substrates. The approximate size of the bands (in nucleotides) is indicated on the right-hand side of eachpanel.S. Prato et al. Analysis of the pIT3 prim–pol domainFEBS Journal...

Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

... the cement apparatus of three adultsessile barnacles, Elminius modestus, Balanus balanoides and Balanus haemri. Mar Biol 7, 239–248.21 Lacombe D (1970) A comparative study of the cementglands ... Bicp-19kM. rosa, B. improvisus and B. albicostatus were collected from Miyako Bay (Iwate), Yodo River (Osaka) and Shi-mizu Bay (Shizuoka, Japan), respectively. RNA and DNAmanipulation was generally ... GAG CCC TGC GTG GC-3¢ and 5¢-AAC TCC GTG GAG AAG AAG AA-3¢ for the first PCR amplification; and 5¢-TGC TGA CCG ACGCGC CTC CT-3¢ and 5¢-GGC AAC ACG GGC GTCACC GC-3¢ for the second PCR amplification....

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... such as the spi-der toxin x-agatoxin [10], C-type natriuretic peptide from the Australian platypus [11], fulicin from Afri-can giant snails [12], and contryphan from conesnails (Table 2). The ... crosspeaks were assigned and inte-grated, with concomitant cycles of structure calcula-tions for evaluation of distance and angle constraintviolations as well as assignments of additional peaksbased ... isunderlined. The polyA signal AATAAA in the 3¢-UTR is also underlined. The cDNA of con-omarphin has been deposited in the Gen-bank database with the accession numberEU048276.Y. Han et al. A D-amino acid-containing...

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... the a- anomer and the b-anomer at equilibrium. The otherpanels show the results of partial hydrolysis of (GlcNAc)6 and (Glc-NAc)5by chitinase G (ChiG). In these panels, substrates display the 60 ... chitinaseshave catalytic domains that are at least as large asthose of the plant enzymes and that may contain atleast six subsites [26,30]. However, the catalyticdomains of ChiG, ChiC and most other ... discussions, and May Bente Brurberg and Svein Horn for helpful discussions. The authorsacknowledge the beamline staff at ID14-EH3 for tech-nical assistance, and the ESRF and the NorwegianResearch...

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... been identified and can be divided intoCHH -A and CHH-B groups [19]. In the crabs Can-cer pagurus, Carcinus maenas and Libinia emarginata and in the crayfishes Procambarus clarkii and Orconec-tes ... were allowed to anneal bymixing equal amounts of each strand, heating to 100 °C for1 min, and cooling gradually to room temperature for3–4 h. Single-stranded RNAs and the annealed RNA(dsRNA) ... 5A) . In the same nitrocellulose membrane, the CHH transcript levels were compared against b-actinprobe and the b-actin transcript was observed as a defined band in the all eyestalk total RNA...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... with a Thermal Cycler Dice RealTime System (TaKaRa Bio Inc.). Forward primers 5¢-CGTTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CACAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi-trogen Corporation) and reverse ... lanes 3–5). A lar-ger amount of the 54 kDa polypeptide was generated from the GmPDIL- 3a mRNA with the AUG2 muta-tion (Fig. 1B, lanes 4 and 5) than from that contain-ing only the AUG1 mutation ... Region, Sapporo, JapanIntroductionSecretory, organelle and membrane proteins are synthe-sized and folded with the assistance of molecular chap-erones and other folding factors in the endoplasmicreticulum...

Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulﬁde isomerases as molecular chaperones for seed storage proteins doc

... 5¢-GACGACGACAAGATGGAGGAATCATCGGAGAAAGAGTTC-3¢ and 5¢-GAGGAGAAGCCCGGTTCAAAGCTCATCTTTTCCTTTTTC-3¢ forGmPDIL-1, and 5¢-GACGACGACAAGATGCTCACCGACGACGAGGACC-3¢ and 5¢-GAGGAGAAGCCCGGTTCATAATTCATCCTTCACATC-3¢ ... sequence data for the cDNA of GmPDIL-1 and GmPDIL-2 and genomicGmPDIL-1 and GmPDIL-2 are available in the DDBJ ⁄ EMBL ⁄ GenBank databases underaccession numbers AB182628, AB185851,AB300660 and AB300661, ... localization of GmPDIL-1 and GmPDIL-2Anti-GmPDIL-1 and anti-GmPDIL-2 sera speciﬁcallyrecognized recombinant GmPDIL-1 and GmPDIL-2,respectively (Fig. 4A, lanes 1 and 4). Anti-GmPDIL-1 and anti-GmPDIL-2...

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