Báo cáo Y học: Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(b1–6)Man-motifs doc
Báo cáo Y học: Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(b1–6)Man-motifs doc
... (Deisenhofen, Germany).Peptide-N4-(N-acetyl-b-glucosaminyl)asparagine amidase A from almond (PNGase A) was from Seikagaku (Tokyo,Japan) and b-galactosidase from jack beans was obtained from Glyco (Upper ... N-linkedcarbohydrate chains from H. pomatia and L. stagnalisrepresent mostly core xylosylated monoantennary anddiantennary complex -type glycans with extended, oftenmultiply branched antennae and ... [42].Methylation analysisPA-oligosaccharides were permethylated and hydrolyzed.Partially methylated alditol acetates obtained after sodiumborohydride reduction and peracetylation were analyzed bycapillary...
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Báo cáo y học: " Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice
... adversely affect the repairing capacity and the defensive mechanism of the GI mucosae that have been damaged during chemotherapy. In this regard, AP was shown to be beneficial to cancer patients ... dampening effect of CY on VEGF expression in the gastric mucosa. These data suggest that AP is a cytoprotective agent which can protect against the cytotoxicity of CY on hematopoietic and gastrointestinal ... Chemicals and Reagents All chemicals and reagents were of analytical grade and were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA) unless otherwise specified. Preparation of Angelica...
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Báo cáo Y học: ORF6 from the clavulanic acid gene cluster of Streptomyces clavuligerus has ornithine acetyltransferase activity potx
... Biosynthetic pathway leading to clavulanic acid. BLS, beta-lactam synthetase (ORF3); PAH, proclavaminate amidino hydrolase(ORF4); CAS, clavaminate synthase (ORF5); CAD, clavaldehydedehydrogenase ... reversible transfer of an acetyl group from N-acetylornithine to glutamate, butnot the formation of N-acetylglutamate from glutamateand acetyl-coenzyme A, nor (detectably) the hydrolysis of N-acetylornithine. ... [6–8].Hydroxylation by clavaminic acid synthase (CAS/ORF5),followed by hydrolysis of the guanidino side-chain catalysedby proclavaminate amidino hydrolase (PAH/ORF4) yieldsproclavaminic acid...
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Báo cáo Y học: Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is an M-type homopolymer doc
... (Millipore) andthen mixed with the matrix solution (a- cyano-4-hydroxy-cinnamic acid) for MALDI-TOF MS analysis.Structure comparison A search of the SwissProt-TrEMBLE database, pairwiseand m ultiple ... saturated with a- cyano-4-hydroxycinnamic a cid ( CHCA) or 2 ,5-dihydroxy-benzoic acid d issolved in water. The mixture of peptide andmatrix was placed on the MALDI stainless-steel plate andallowed ... ourknowledge only s pleen ferritin from another A ntarcticteleost, Gymnodraco acuticeps, has been partially character-ized; it is an H -type homopolymer, as i ndicated by the N-terminal amino-acid sequence,...
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Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc
... men and three women (all Italian),ranging from 49 to 79 years of age (mean age, 64.8 ±7.8 years). None had any apparent distant metastasis, andnone had been previously treated for HCC. The ... growth and metas-tasis. As pharmacological regulation of uPA andc-met has proved to be a challenge in cancer, thisregulatory mechanism may potentially be a new tool with which to alter the expression ... reproducibility. The expression of uPA and c-met mRNAs in the celllines shown in Fig. 2 was evaluated using a TaqMan GeneExpression Assay (Applied Biosystems). GAPDH was usedas an internal standard. The...
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Báo cáo y học: " Factors affecting the long-term response to tacrolimus in renal transplant patients: Pharmacokinetic and pharmacogenetic approac"
... amount of CYP 3A5 protein accurately as it can be affected by other factors, genetic (expression of m-RNA, stability of CYP 3A5 protein, dual metabolic pathway via CYP 3A4 and CYP 3A5 , linkage disequi-librium ... be a more accurate me-thodology for the analysis of such data but it is more demanding in the quantity of available data. In the LR analysis, the kinetic parameters were modeled based on sex, ... CYP 3A, the primary subfamily of the cytoch-rome P450 (CYP) enzymatic system, is responsible for the metabolism of tacrolimus [10]. Specifically; tacro-limus is mainly metabolized by CYP 3A4 ...
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Báo cáo y học: "Foundation for the Community Control of Hereditary Diseases, Budapest, Hungary"
... folds, ocular hypotelorism, preauricular tags and pits, low-set ears, simian crease, clino- and camptodactyly, partial syndactyly between toes 2 and 3, hydrocele, umbilical hernia, sacral dimple, ... prevalence of congenital abnormalities is used. Of course, the total prevalence of congenital abnormalities depends on the spectrum of congenital abnormalities evaluated, the period of study ... characteristics of the study population, etc. In Hungary the total prevalence of congenital abnormalities was 66.83 per 1000 informative offspring in the 1980s and within this, the total rate...
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Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"
... Criteria for Assessing Applicability to Human Germline Gene Therapy An ideal gene transfer system in the context of human germline gene therapy would have the following features: (a) the ability ... rates) would there be any point in attempting the technique with humans – but the necessary data on efficiency could only come from actual attempts with humans. Another problem associated with ... cells are simply incubated in a DNA-containing solution. As a potential tool for genetically manipulating animals, sperm-mediated gene transfer (SMGT) has the advantages of simplicity and cost-effectiveness,...
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Báo cáo y học: "Update on the treatment of ocular toxoplasmosis"
... demonstrate the efficacy of daraprim in the therapy of ocular toxoplasmosis. But the out-come was measured 4 weeks after initiation of ther-apy. Dihydrofolate reductase inhibitors (DHFR) have ... of ocular toxoplasmosis. The efficacy of the drug was reported in cerebral toxoplasmosis in AIDS patients. The dos-age used by Rothova et al was 250mg a day or 500mg every other day and therapy ... tablets 750mg QID have been given for the treatment of Toxoplasma retinochoroiditis. Azithromycine is an alternative to classical therapy was proposed as an alternative therapy in the treatment...
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Tài liệu Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide doc
... vertebrateorigins. Science 298, 2157–2167.8 Satou Y, Yamada L, Mochizuki Y, Takatori N,Kawashima T, Sasaki A, Hamaguchi M, Awazu S,Yagi K, Sasakura Y et al. (2002) A cDNA resource from the basal ... [48].Measurement and analysis of AChE activityand inhibitionAcetylcholinesterase activity was measured according to the method of Ellman et al. [49] as modified by Doctoret al. [50] in 100 mm NaHPO4, ... [53].Apparent sedimentation coefficients were calculated relativeto the sedimentation of catalase (11.3S). Data were plottedas fractional activity of total AChE activity on the gradientas a function...
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