Báo cáo khoa học: Genomic structure, expression and characte

Báo cáo khoa học: Genomic structure, expression and characterization of a STAT5 homologue from pufferﬁsh (Tetraodon ﬂuviatilis) ppt

... TGG AAC GAC GG gtaagggaac 172 ttttttttag A GCC ATA CTG Gly593 (2) 15 143 AAC AAA GCA G gtatattcag 434 atgtttccag GT GAG AGA ATG Gly640 (1) 16 156 CCG CCC CTT T gtaagcaacc 139 tcacctaaag CC AAA ... ttttctacag C ACC TTT ATT Ser331 (2) 9 80 AAC ACA AGG AA gtaagttcaa 535 tctgccacag T GAA AGC AGT Ans391 (2) 10 88 TTC AGG AAC ATG gtgagtgcct 306 atccttgtag TCC TTG AAG Met420 (3) 11 123 TTT CAA GTG...

Ngày tải lên : 31/03/2014, 07:20

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Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulﬁde isomerase from Conus marmoreus pdf

... Subsequently, insulin was added, and a stable nonenzymatic rate was recorded. Finally, PDI was added, and the total NADPH oxidation rate was recorded. The oxidase and isomerase activities of PDI were mea- sured ... reaction times, the refolding mixture was acidiﬁed and immediately analyzed by RP-HPLC. The amounts of native and linear tx 3a were calculated from their elution...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

... min at 72 °C with a 5 min preheat at 95 °C and a 10 min ﬁnal extension at 72 °C using primers, S1 (5¢-GCAAATGCAACTGGA AGCGG-3¢)andA1(5¢-ACAGCCTGCTAGCAAAGA GG-3¢) for ampliﬁcation of HRT1, and ... Wititsuwannakul 4 , Seiji Takahashi 1 , Atiya Rattanapittayaporn 4 and Tanetoshi Koyama 1 1 Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Ja...

Ngày tải lên : 07/03/2014, 21:20

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Báo cáo khoa học: Gene cloning, expression and characterization of avian cathelicidin orthologs, Cc-CATHs, fromCoturnix coturnix pdf

... MEGFFWKTLLVVGALAIAGTSSLPH.KPLIYEEAVDLAVSIYNSKSGEDS.LYRLLEAVSPPKWD.PLSES L L L L L L L L L L L L L L L L L L L L L L L Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A N N N N N N N N N N N N N N N N N N N N N N N R R R R R R R R R R R R R R R R R R R R R R R L...

Ngày tải lên : 28/03/2014, 23:20

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Báo cáo khóa học: Localization, puriﬁcation and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot

... Localization, puriﬁcation and properties of a tetrathionate hydrolase from Acidithiobacillus caldus Zhanna Bugaytsova* and E. Bo¨ rje Lindstro¨m Department of Molecular Biology, Umea ˚ University, ... interference of ammonium sulfate with the HPLC- assay of tetrathionate was also tested. The height of the tetrathionate peak decreased and a shoulder appeared in the HPLC-c...

Ngày tải lên : 07/03/2014, 14:20

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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

... (5¢ﬁ3¢) FLAG epitope GAC TAC AAG GAT GAC GAT GAT AAG DYKDDDDK Library template (FLAG-negative) TTG GGG CTG GAT GAC GCG GAT AAG L GLDDADK Randomization NNS NNS NNS GAT GAC NNS GAT AAG XXXDDXDK Selected ... proteins carrying a functional FLAG epitope from an excess of FLAG-negative com- petitors and from a large library of random variants was also undertaken by plasmid display, to...

Ngày tải lên : 30/03/2014, 20:20

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Báo cáo khoa học: Neuropeptide Y expression and function during osteoblast differentiation – insights from transthyretin knockout mice potx

... 5¢-GTGGGCCGCTCTAGGCACCAA-3¢ and 5¢-CT CTTTGATGTCACGCACGATTTC-3¢; for HPRT, 5¢-GT AATGATCAGTCAACGGGGGAC-3¢ and 5¢-CCAGCA AGCTTGCAACCTTAACCA-3¢; for GAPDH, 5¢-ACTCC ACTCACGGCAAATTC-3¢ and 5¢-CCTTCCACAATGC CAAAGTT-3¢; ... 5¢-TCTGATGAGACCGTCACTGC-3¢ and 5¢-TC TCCTGGCTCTCTTTGGAA-3¢; for PAM, 5¢-CCTGG GGTCACACCTAAAGA-3¢ and 5¢-TGTAAGGACACAC CGGAACA-3¢; for Y1 receptor, 5¢-CTCGCTGGTTCTCA T...

Ngày tải lên : 15/03/2014, 09:20

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Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... 710–728 RpCAtrRq a TCA CAA ATG TCC AGT GCC AGT T 757–778 Full-length sequencing of RpCAbr RpCAbrF TAC AAG GAT GCC ATT AGC 613–630 RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839 RpCAbrR2 AGA GCA GCA GAC CTT ACG 706–723 RpCAbrR3 ... 706–723 RpCAbrR3 GTT ACT TCC GCA GCT AGG 466–483 Probe ampliﬁcation for FISH RpCAbrF TAC AAG GAT GCC ATT AGC 613–630 RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839 RpCAtrFp...

Ngày tải lên : 18/02/2014, 16:20

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Báo cáo khoa học: Identiﬁcation, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot

... I-D-AEA-K R-DD-QNTIVK-VGATAASASA 122 A Active site R101 B N R I/M HvTrxh1 HvTrxh2 Ta a Os a Os b Td Ta b Ta c Ta d Hv a Sc Lp Hb Os c C K N A Zm Os e Os d Lc Pc Ata Atb Atc Atd Ate Atf Atg Bna Bo Bnb Bra Brb Pm Fe Cm Nta Pt Ath Ati Rc Ntb Ntc Pp Psa Psb Psc Psd * Fig. ... puriﬁed protein was missing methionine and alanine from the N-terminus. This was conﬁrmed by the N-terminal...

Ngày tải lên : 23/03/2014, 17:22

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Báo cáo khoa học: Coexpression, puriﬁcation and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx

... GGGTCTAGAATGGAAAAAGATCTACAGTTAAGA 33 CCGGAATTCTTATTTCCCTTCTCTCATCTC 40 GCGCGCCATGGAAAAAGATCTACAGTTAAGA 41 GGGGGATCCCCATAATCCACTCCACCTGCTAAA 44 GGGGGGGATCCT CATTATTTCCCTTCT 66 AATACCGCCATGTATAATATCTATTACTTC 67 ... AATACCGCCATGTATAATATCTATTACTTC 67 GTAATAGATATTATACATGGCGGTATTGAA 75 GGGGGGGCCATGGAAAGAGCAGACGATTT 4294 H P. Chen et al.(Eur. J. Biochem. 271) Ó FEBS 2004 assay k it was obtained f ro...

Ngày tải lên : 30/03/2014, 15:20

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