Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx
Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx
... The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II Kangcheng ... 4. The unfolding of the 33-kDa protein induced by GdmCl in the presence of different sucrose concentrations. Other conditions as inFig. 1.Fig. 5. The unfolding of the 33-kDa protein induced by ... role in the sucrose or glycerol protein protection against pressuredenaturation.Effects of sucrose and glycerol on the kinetics of the pressure induced protein unfolding and refolding The unfolding...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
... cuticular proteins [35,36]. The abundance of Hisresidues in the basic QH proteins therefore raises the possibility that these proteins play an important role inmaintenance of the integrity of the ... individuals[1–3]. The mechanical properties of the cuticle dependon the content of chitin, the microarchitecture of chitinfilaments, and the interaction between the chitin-filament system and cuticular proteins. ... role as the first barrier against inva-ding pathogens. We extensively determined the sequences of horseshoe crabcuticular proteins. Proteins extracted from a part of the ventral side of the cuticle...
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Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx
... treatment of the collected pools to determine the distribution of sialicacid. Next, we completed online MS ⁄ MS analysis of the major species; some of these species were furtheranalyzed by TOF ⁄ TOF ... protons of GlcNAc, and the H3-equitorial and H3-axial protons of N-glycolylneuraminic acid[23–26]. The 1H-NMR spectrum of cRBCs within the region of interest is shown in Fig. 3 and the list of important ... FEBSdetermined by the label and independent of the attri-butes of the glycan to which it is attached. Accord-ingly, we aimed to determine the amount of individualcRBC glycans by HPLC through the use of...
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Tài liệu Báo cáo khoa học: Elementary modes analysis of photosynthate metabolism in the chloroplast stroma ppt
... many of the reactions of the regenerative limb of Calvin cycle with the addition of transaldolase. The function of this pathway is lessclearly defined, and may be more varied than that of the Calvin ... attention to the second of these, and consider the likely outcomes of changing the topology of stromal metabolism in the dark.Thus it is that the goals of this investigation are three-fold. By applying ... modelling stem primarily from the uncertainty in the definition of the kinetics, both in terms of the form that the rate equation should take, and in the values to beassigned to the associated kinetic...
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Tài liệu Báo cáo khoa học: A comparative analysis of the time-dependent antiproliferative effects of daunorubicin and WP631 pdf
... USA) at the ÔServeis Cientifico-TecnicsÕ of the University of Barcelona,using the 488 nm line of an argon laser and standard opticalemission filters. Percentages of cells at each phase of the cellcycle ... behavior of daunorubicin and WP631,we compared the intracellular accumulation of these com-pounds in Jurkat T cells overtime. We also examined the rate and overall level of cell killing by either ... areshown as a percent of the cells in untreated control cells and represent the means of three independent experiments. The arrow indicates the rapid decrease in the number of viable cells after...
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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx
... that stabilize the binding loop. The hydrophobic core is organized differently in the twomolecules either b y a Phe of the ®rst or by a Trp of the thirdb strand. The natural form of PMP-C is fucosylated ... those of small insect toxins, defensins and some otherproteinase inhibitors [15±17]. The c ommon feature is the presence of three disul®de bridges and three b strands.However, the topology of the ... ifference i n the internal ¯exibility of t he molecules. The rigid nature of the second b strand is consistent with the presence of the indole r ing of Trp25 bridging strands 1, 2 and 3. It should...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... envelope of the bound protein. The percentage of protein bound to RNA was the ratio between the value of bound protein and the total protein present in the sample. The relative percentage of binding ... semiquantitive assessment of the relative levels of toxicity of the different mutants was derived by comparing the number of colonies and growth of the spots at the differentlevels of dilution.CD spectroscopyCD ... (1991) The kis and kid genes of the parDmaintenance system of plasmid R1 form an operon thatis autoregulated at the level of transcription by the co-ordinated action of the Kis and Kid proteins....
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Báo cáo khoa học: Structure–function analysis of the filamentous actin binding domain of the neuronal scaffolding protein spinophilin pot
... Disorders and Stroke to WP. The content is solely the responsibility of the authors and does not necessarily represent the of cial views of the National Institute of Neurological Disorders and Stroke ... spectra of the spinophilin ABD constructs(Fig. 2C), which enables rapid analysis of the overallsecondary structure content of proteins. The CD spec-tra of residues 1–154, 1–221 and 1–305 of spinophilinwere ... regulation and maintenance of dendritic spine morphology is funda-mental to our understanding of the functions of the central nervous system. The molecular details that underlie the regulation of spine...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... by a much slower phase, which is believed toresult from slow release of the cleavage products from the protein. The amplitudes of the initial burst (2.78 and 2.83 nM) gave a ratio of DNA /protein ... Cl–ions, as well as the inclusion of 0.1 mgÆmL)1BSA did notincrease the stability of the protein and slowed the reactionrate (data not shown). The inclusion of 5–10% glycerol increased stability ... the pro-teinÆDNA complex. Furthermore, the fact that the protein was saturated with the same amount of either substrate(Fig. 4) argues against the trivial explanation that only afraction of...
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Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot
... involves the formation of a salt bridge between the a-amino group of the substrate and the carboxylic group of Asp235 and a hydrogen bond to the carbonyl atom of Ser330. The d-aldehyde group of the ... pair of A domains and PCPs and catalyze the formation of the peptide bond between the upstream amino acyl orpeptidyl moiety tethered to the phosphopantetheinylgroup and the free amino group of the ... adenyl-ated at the d-carboxylate rather than the a-carboxy-late and that the a-amino and a-carboxyl groups of the substrate bind at the bottom of the pocket inter-acting with the Arg239 and Glu322...
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