Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

... (2005) 5217–5229 ª 2005 FEBS 5229 Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle Anna Penelova1, Larry ... vari-ations.Results Steady-state levels of cyclin mRNA in the cell cycle In a ﬁrst series of experiments we determined whether mRNA steady-state levels of cyclins and several cell cycle- related mRNAs change ... EL4 cells in log-arithmic growth.No major variation in the mRNA half-life of cyclin mRNAs in the cell cycle In order to test whether changes in mRNA steady-state levels correlate with any changes...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudorcoding sequences were joined to the C-terminal region of the GFP or RFP coding ... transcribed from theseconstructs in vitro was injected into medaka eggs in the one -cell stage to visualize the localization and to mea-sure the mobility of the components in the PGCs. The medaka ... movement in the granules and diffused in the cytosol of HeLa cells, just as in the medaka embryo. The other twogene products, Nanos and Tudor of the medaka, which are known as con-stituents of the...

Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

... proteins shows that each of the domains does contribute to the accumulation in cyto-plasmic foci. Similar to what we found for Lsm8p,there appears to be a hierarchy to the contribution of the ... Smdomains or, in the case of Lsm8p, made point muta-tions in putative RNA-binding residues.We investigated the cellular localization of GFP-tagged versions of these proteins, as well as their ... the latter, indicating that in the absence of an N-terminaldomain distinct localization is lacking. Finally, the Lsm1p Sm domain by itself (LsmDN1DCp) accumu-lates in both the nucleus and the cytoplasmic...

Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot

... dashes. The active site (YTVAK) and the cofactor-binding motifs aremarked in bold. The D43, T67 and A69 residues, involved in cofactor speciﬁcity, are indicated by asterisks. Flanking the N-terminal ... that the N-terminal segmentwould be sufﬁcient to target and anchor the protein to the ER membranes. Furthermore, we analyzed the contribution of the C-terminal end to ER localization.We fused the ... the addition of cofactor and carried out for 15 min at 37 °Cin0.5 mL. The amount of protein used in the reaction mixturewas 15 lg. The reaction was terminated by the addition of an equal volume of cold...

Báo cáo khoa học: "Analysis and Synthesis of the Distribution of Consonants over Languages: A Complex Network Approach" pptx

... corresponding to the nodes in the set VLand the other corre-sponding to the nodes in the set VC.Degree distribution of the nodes in VL: Fig-ure 2 shows the degree distribution of the nodes in ... originated. In this paper, we mainly dealt with the occur-rence principles of the consonants in the invento-ries of the world’s languages. The work can be fur-ther extended to identify the ... work. Rather, here we try to investigate the ef-fect of the exact distribution of the language inven-tory size on the nature of the degree distribution of the consonants through a synthetic...

Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... thereforeattempted to deﬁne further the features in the C-ter-minus of 4E-BP1 that are involved in its binding to raptor. The data for the other truncation mutantsshown in Fig. 1C indicate that other regions ... that the N-terminus, containing the RAIP motif, and a more C-terminal region (out-side the ﬁnal 20 residues, i.e. other than the TOSmotif) are involved in binding to raptor. Although the TOS ... sites. The interaction of these proteins with raptor, via their TOSmotifs, promotes their phosphorylation by mTOR in vitro. Both of these types of protein are implicated in controlling the translational...

Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

... in the maturation of the enzymes. Most o f them a ct pleiotropically in the synthesis of all four hydrogenases, in particular in the synthesis and insertion of the metal centre [6,7]. Two of the products ... Klenowenzyme to remove the protruding ends and by cloning into the SmaI r estricted vector pT7-7. I n this w ay the GTG startcodon of the hypE gene was out of frame relative to an A TG of the vector. The ... using carbamoylphosphate as asubstrate and transferring the carboxamido moiety in anATP-dependent reaction to the thiolate of the C-terminalcysteine of the HypE protein yielding a protein-S-carbox-amide...

Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

... byPCR to conﬁrm the presence of the RIP/lectin codingsequence in the tobacco genome. Only those plants thatwere positive in the PCR analysis were withheld for further analysis. Expression of the ... convertedinto SNA-II and only 5% in SNA-V, the SNA-V-expressingtobacco plants contained little or no rSNA-II, indicatingthat in tobacco the precursor of rSNA-V is exclusivelyconverted into the ... leaf protein. Allother lines exhibited markedly lower expression levels of recombinant RIPs/lectins, varying between 0.03% and1.7% of the total soluble protein. To verify whether the rRIPs...

Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf

... Dimerizationmarkedly affects the binding ability of the subunits. Notably, heparin andmannose 6-phosphate binding abilities of PSP-II are abolished, indicatingthat the corresponding binding sites may be ... (Fig. 1A) along with an increase in ellipticity below 250 nm. These changes reﬂect the loss of tertiary structure of the protein. Monitoring of the decrease with temperature of the ellipticity at ... Variation in ellipticity at 268 nm with temperature monitored in the absence (s)orin the presence of 0.5 (n)or4(h)mM Zn2+. The continuous lines correspond to the ﬁt of the experimental data to a...

Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein pot

... need other factors, apart from theirmature amino acid sequence, in order to fold efﬁciently andrapidly into the native form in vivo. The nature of thesefactors, whether they are found in their ... [18,19]. The extremelyineﬃcient folding of PCI in vitro [18], together with the presence of the above-mentioned long pro-sequences,suggest a possible involvement of them in the in vivo folding of ... PCI.Here, using different protein variants, we have investi-gated the role of both pro-sequences in the in vitro refolding of PCI, together with studies of the in uence of the N-terminal pro-region...

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