Báo cáo khoa học: Protein glycation in Saccharomyces cerevisiae Argpyrimidine formation and methylglyoxal catabolism ppt

... Weinvestigated methylglyoxal metabolism and protein glycation in Saccharo-myces cerevisiae. Using a speciﬁc antibody against argpyrimidine, a markerof protein glycation by methylglyoxal, we ... cells [21]. Protein glycation by methylglyoxal in yeast, monitoredby argpyrimidine formation in proteins, was evaluated in a set of null mutant yeast strains for genes involved in MGO detoxiﬁcation: ... of argpyrimidine formation in yeast. (A) Time course of argpyrimidine formation in single gene deletion strains. Yeaststrains and growth time are shown. (B) Time course of argpyrimidine formation...

Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx

... CaM-bindingdomains, and contains at least one conserved potentialCaM-binding motif [46]. Additionally, the binding ofCaM at the C-terminus and the binding of NADPH atthe extreme N-terminus ... domains are degenerate 34-amino acidrepeats forming anti-parallel a-helices known to beinvolved in an array of protein protein interactions(generally with non-TPR proteins) [41]. Both domainsare ... N-terminal half ofthe protein, including the dehydrogenase domain. Spe-ciﬁc binding of the FNR is mediated by a unique seriesof proline/serine-rich repeat motifs located in theC-terminus....

Báo cáo khoa học: Autonomous oscillations in Saccharomyces cerevisiae during batch cultures on trehalose pot

... short-period-sustained oscillation of Saccharomyces cerevisiae. Yeast 12, 673–682.8 Satroutdinov AD, Kuriyama H & Kobayashi H (1992)Oscillatory metabolism of Saccharomyces cerevisiae in continuous ... tre-halose in whole yeast cells. Anal Biochem 248, 186–188.34 Hans MA, Heinzle E & Wittmann C (2003) Free intra-cellular amino acid pools during autonomous oscilla-tions in Saccharomyces cerevisiae. ... However,sustained glycolytic oscillations have been observed in intact cells under speciﬁc conditions [4,5]. Their fre-quency is around 1 min in intact cells, and the syn-chronizing agent is...

Báo cáo khoa học: Protein transport in organelles: The Toc complex way of preprotein import pdf

... their role in precursor protein recognition and receptor protein function.Toc159 was ﬁrst identiﬁed by chemical cross-linking atboth the reversible and stable binding stages of prepro-tein import ... preproteins. AtToc12 (12)exposes a J-domain (J) into the intermembrane space and has arole in anchoring Hsp70, thereby assisting in the transfer of prepro-teins to the translocase at the inner ... FEBSaccumulation of many proteins involved in photosyn-thesis (termed ‘photosynthetic proteins’), suggestingthat both atToc33 and atToc159 are involved in theimport of photosynthetic proteins. However,...

Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx

... for proteinaceous insertion machinery. Other single-spanning proteins have also been suggested to use thismembrane integration route, including the photosys-tem II subunits, PsbW and PsbX. In ... undergoes conformational rearrange-ment during active protein transport, with the amphi-pathic helix and C-terminal tail interacting only in response to conditions leading to protein transport[30]. ... immediate vicinity ofthe twin arginine motif, whereas Hcf106 cross-linksless strongly to residues in the hydrophobic core and the early mature protein [32]. Binding of the precursorcan occur in the...

Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt

... mitochondrial and chloroplastictargeting signals in terms of arginine and serine con-tent (i.e. not as high as in mitochondrial targetingsignals) and may be slightly enriched in hydrophobicresidues. In ... raises interestingquestions for inter-organelle communication. A greaterunderstanding of the process of dual targeting mayprovide useful insights into the targeting of location-speciﬁc proteins ... here[17,18]. Instead, we provide an overview the signals,proteins, receptors, sorting of dual-targeted proteins and why dual targeting may occur. Finally, an outlineof the future challenges in this...

Báo cáo khoa học: Nucleosome positioning in relation to nucleosome spacing and DNA sequence-speciﬁc binding of a protein doc

... ª 2007 FEBSNucleosome positioning in relation to nucleosome spacing and DNA sequence-speciﬁc binding of a protein Rama-Haritha Pusarla*, Vinesh Vinayachandran* and Purnima BhargavaCentre for ... thenucleosome-spacing effects on the bulk chromatin.The distance between protein- binding sitesdecides nucleosome positioningPositioning of ﬁve nucleosomes in a single register as aresult of the binding ... the number of bindingsites, and the distance between the binding sites.DiscussionNucleosome spacing and positioningwith changing ionic strengthNucleosomes are nucleoprotein complexes formed...

Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx

... strain in glucose med-ium (Figs 10A and S4), indicating that, although theseproteins show sequence similarity in the C-terminalportion, divergences in the remaining sequence of the protein ... two-hybrid assaysFusion proteins with either lexA DNA-binding domain(BD -protein) or Gal4p transcription activation domain(AD -protein) were expressed in the host strain L40 [39],which has two ... identiﬁed in the nucleolus, an indication that this protein could be involved in ribosome biogenesis. Here we show that Utp25pinteracts with the SSU processome proteins Sas10p and Mpp10p, and affects18S...

Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx

... were washed off and bound preproteinseluted with 250 mm imidazole containing buffer. In every case, precursor protein was detected in the imid-azole eluate, indicating a direct interaction withToc34DTM. ... Toc75 preprotein contains a bipar-tite targeting signal. The N-terminal domain is respon-sible for chloroplast targeting and translocationinitiation. Translocation is halted when this domain istranslocated ... character-istics of the intermembrane space proteins Tic22 and MGD1, in vitro import experiments into isolatedchloroplasts were performed using35S-labelled precur-sor proteins. Both pTic22 and pMGD1 were...

Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf

... reaction.AbbreviationsAPPBP1, APP binding protein 1; CtBP, C-terminus binding protein; CTD, C-terminal domain; E1, activating enzyme; E2, conjugating enzyme;E3, ligase; HECT, homologous to E6AP C-terminus; IR, internal ... in Nedd8, and Arg72 in ubiquitin (highlighted in yellow in Fig. 2C). Matching E1 residues are Thr149 in uba2, Arg190 in uba3 and Gln608 in uba1 (high-lighted in purple in Fig. 3E). Interestingly, ... migration inhibiting factor 2; Nedd8, neural cell-expresseddevelopmentally down-regulation protein 8; Pc2, polycomb protein 2; PIAS, protein inhibitor of activated STAT; RanBP2, Ran bindingprotein...

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