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Báo cáo khoa học: Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus pot

Báo cáo khoa học: Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus pot

Báo cáo khoa học: Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus pot

... Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus Hyo-Il ... Competitiveinteraction of component enzymes with the peripheral subunit-binding domain of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus: kinetic analysis usingsurface ... Perham, R.N. (2002)Thermodynamic analysis of the binding of component enzymes in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Protein Sci. 11, 1091–1100.13....
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Báo cáo khoa học: Interactions of imidazoline ligands with the active site of purified monoamine oxidase A potx

Báo cáo khoa học: Interactions of imidazoline ligands with the active site of purified monoamine oxidase A potx

... so the nitro-genous group of agmatine must be within 3 A˚ of N5 of the isoalloxazine ring of the flavin. Agmatine, however,binds poorly to MAO-A, giving only 50% inhibition of the oxidation of ... and the resultinginhibition. The use of purified enzyme simplifies the system for kinetics and also allows observation of the spectrum of the flavin cofactor in MAO-A. The subtlechanges in the ... and the values for 2-BFI and idazoxan aregiven in Table 1.Evidence for the proximity of these ligands to the flavin in MAO-A comes from the in uence of the lig-and on the redox properties of the...
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Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf

Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf

... protein; GST, glutathione S-transferase;HD, Huntington’s disease; HIP1, Huntingtin interacting protein 1; HIPPI, Huntingtin interacting protein 1 protein interactor; Htt, Huntingtin;IOD, integrated ... decrease in the intrinsic fluorescence of GST–HIPPI protein, indica-ting binding of the protein with this mutated motif(Fig. 1B, panel II). The apparent dissociation constant(Kd) of this binding ... addition of the upstream sequences of the caspase-1 gene (717 bp) to the N-terminal domain (without the pDED domain) of HIPPI did notdecrease the fluorescence intensities determined byexciting either...
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Báo cáo khoa học: Interactions of elongation factor EF-P with the Escherichia coli ribosome doc

Báo cáo khoa học: Interactions of elongation factor EF-P with the Escherichia coli ribosome doc

... which is a precursor of hypusine in the eIF-5A protein [32]. The model in Fig. 5, in which the orientation of the spermidine residue of EF-P is facing the mRNA-binding pocket of the 30S subunit is ... importantto the function of the EF-P protein is the fact that the interactions of the molecule are in close proximity to the peptidyltransferase center of domain V near the positions of the A-site ... Streptomycin is a potent inhibitor of EF-P-medi-ated synthesis of peptide bonds [12]. Streptomycininhibits translation by increasing the error rate of syn-thesis and interfering with the proofreading...
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Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf

Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf

... receptors, including human RXR (which is the ortholog of USP) have shown that an area on the surface of the ligand binding domain involving the C-terminus of a-helix 3, a-helix 4, and the N-terminus of ... binding byeither purified recombinant dUSP, or by components of Sf9nuclear extracts. Binding conditions for recombinant dUSPwere 1.0 lg of dUSP, 1 lg dIdC, 3 lLof5· binding buffer(1·:10mm of ... under the same conditions, transfection of increasing amounts of USP-expressing plasmid doesnot cause an increase in response to 20OHE alone, butinstead causes a decline in the level of induction...
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Báo cáo khoa học: Interactions of the antimicrobial b-peptide b-17 with phospholipid vesicles differ from membrane interactions of magainins potx

Báo cáo khoa học: Interactions of the antimicrobial b-peptide b-17 with phospholipid vesicles differ from membrane interactions of magainins potx

... calori-metry results using magainin II with those of the morepotent magainin analog, [Ala8,13,18]-magainin II amide[30]. We find that the peptide-induced changes in the transition of DPam2PtdEtn are ... hydration of the headgroup and the higher energy cost of bending the membrane. In addition, the presence of the anionic lipid PtdGro in thesemixtures is important as this is a major component of prokaryotic ... magainin IIand for [Ala8,13,18]-magainin II amide (data not shown). In a toroidal pore there are two types of curvatures to beconsidered, i.e. one in the plane of the membrane and the other...
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Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

... protein H appear to be involved in the interaction as the binding of 125I-labelled DS was reduced to isogenicderivatives of the AP1 strain lacking either of these surfaceproteins (Fig. 2). The ... charged sulfate groups of the GAGchains and positively charged regions of the ligand. Typi-cally, the heparin-binding domains of known GAG-bindingproteins are rich in basic amino acids that are ... 3D). In previous studies, we havedefined the binding regions in the M1 protein for fibrinogento the NH2-terminal half (A–B3), for IgG to the central S domain, and for human serum albumin to the...
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Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt

Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt

... pro-cessed into fragments by proteolysis. Recently, the Brichos domain has been found also in other proteins,including a protein (TFIZ1) that binds trefoil domains[13]. The Brichos domain may be involved ... part of proSP-C (CTC)].CTC contains a  100-residue Brichos domain, cov-ering the region from residue 94 to the C-terminal end. The name Brichos refers to the fact that the domain was initially ... involved in foldingand processing of the precursors and in binding toother polypeptides [12,14]. The structural propertieshave not been experimentally investigated for any Bri-chos domain, and...
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Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf

Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf

... 5.1-fold,respectively.Increased protein level of TFAM induced byD-GalTo further understand the expression of TFAM protein in the inner ear, western blot analysis was performed. The protein levels of TFAM in the ... in mutant mtDNA harboring the com-mon deletion. By applying the specific probe, we caneven detect the presence of the common deletion in the inner ear in the control group. The proportions of ... explore the possible mechanisms involved in the development of presbycusis. In the present work, we demonstrated significantlyincreased levels of the mitochondrial common deletion in the inner ear of...
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Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx

Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx

... within the mature 18S rRNA (Fig. 7C, asterisk). These resultsfurther indicate the involvement of Utp25p in the earlysteps of processing of pre-40S.To determine whether Utp25p might associate in ... further indicating thatthis protein is associated only with the early pre-rRNAs. This is in accordance with the hypothesis of the involvement of Utp25p in the early cleavages of the 35S pre-rRNA, ... C-terminalportion, divergences in the remaining sequence of the protein would render hUtp25p nonfunctional and ⁄ orunstable in yeast.To analyze the possibility that the C-terminalDUF1253 domain...
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