Báo cáo khoa học: The Thermoplasma acidophilum Lon protease has a Ser-Lys dyad active site pot
Báo cáo khoa học: The Thermoplasma acidophilum Lon protease has a Ser-Lys dyad active site pot
... wild-type Lon the protease domain might influ-ence the activity of the ATPase domain.Keywords:AAA+ protease; archaea; Lon( La) endopepti-dase; Lon (La) protease; Ser-Lys dyad. Endopeptidase La (EC ... [21](Fig. 1 A, B). TaLon encompasses an N-terminal ATPaseassociated with various cellular a ctivities ( AAA+domain)and a C-terminal protease domain, but lacks the N-terminal a- helical domain inherent ... acterial Lon protease and its homologues i n eukaryal organelles aresoluble, the archaeal c ounterpart is membrane-attached[13]. Bacterial LonB proteases, like archaeal Lon, lack the LAN domain...
- 5
- 298
- 0
Tài liệu Báo cáo khoa học: The double-stranded RNA-binding motif, a versatile macromolecular docking platform doc
... in RNA helicase A (RHA), which has a DEXHhelicase domain for the unwinding of duplex of nucleicacids [11], and in RNaseIII and the related Dicer ⁄Drosha enzymes of the RNAi ⁄ miRNA pathway, ... whichcarry an RNase domain for dsRNA cleavage and pro-cessing [12–14]. A second group of dsRBM-containing proteins donot carry an identifiable catalytic domain. Examplesare the staufen and Xlrbpa ... Sequence alignment for a set ofdsRBMs along a cartoon for the basic abbbafold (B) that dsRBM adopts. The alignmentwas generated byCLUSTALW [60] and manu-ally optimized. The species of origin, nameand...
- 9
- 542
- 0
Tài liệu Báo cáo khoa học: "THE REPRESENTATION OF INCONSISTENT INFORMATION IN A DYNAMIC MODEL-THEORETIC SEMANTICS" ppt
... ABSTRACT Model-theoretic semantics provides a computationally attractive means of representing the semantics of natural language. However, the models used in this formalism are static and are ... management of the relationship is external to the model. This mechanism has the disadvantage that it involves high overhead - the denotational relationships must be repeatedly verified, and ... evaluates a formula with respect to a model has been augmented with a table that contains each ~-expression and the ima6e of its denotation in the current stage of the dynamic model. When the domain...
- 3
- 394
- 0
Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCAVps4–TRP F TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGAVps4–TRP R TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAAVps4–RDF F GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTGVps4–RDF R CACGTTAATCAATTGACTAAGTGAACTGTTCTTGCTTCTable ... the AAA superfamily typically containone or two ATPase domains that assemble into one ortwo stacked hexameric rings. The ATPase catalytic site is located at the interface between adjacent ATPasedomains ... D,Uchimura S, Nara A, Yoshimori T, Hayashizaki Y,Kawai J, Ishidoh K et al. (2004) Mammalian class EVps proteins, SBP1 and mVps2 ⁄ CHMP 2A, interactwith and regulate the function of an AAA-ATPaseSKD1...
- 23
- 490
- 0
Báo cáo khoa học: The male seahorse synthesizes and secretes a novel C-type lectin into the brood pouch during early pregnancy pdf
... andXhoI sites at either end, using the following primers:forward, 5¢-CGCGGATCCTGGTCTTTCCAAAATATTCAGGCCA-3¢ and reverse, 5¢-GTCCTCGAGGTACATCACATCTCTGAT-3¢. After digestion, the PCR-amplifiedfragment ... levels afteruptake of the eggs, but that its levels drop off after the larvae are hatched and as the juvenile seahorses arepreparing to leave the pouch. This suggests the passageof a signal between ... h, and D595readings takenevery 30 min to assess the rate of bacterial growth. The D valueswere calculated relative to the initial readings in the same samples.An asterisk denotes mean values...
- 15
- 379
- 0
Báo cáo khoa học: The binding of IMP to Ribonuclease A pptx
... accessible areas were calculated with the program naccess [56]. Atomic coordinates and the X-rayamplitudes of the RNase A IMP, and RNase A AMP,complexes have been deposited in Research Collaboratoryfor ... 3989 has been attributed to the lattice contacts that limitaccess to the active site of mol B in the asymmetricunit.In all free RNase A structures reported so far the side chain of the catalytic ... for the RNase A AMP complex is 532 A ˚2and 540 A ˚2inmol A and mol B, respectively. The shape correlationstatistic Sc [33] is 0.77 for the association of AMP to the active site of RNase A. Comparative...
- 14
- 360
- 0
Báo cáo khoa học: The Mycobacterium tuberculosis ORF Rv0654 encodes a carotenoid oxygenase mediating central and excentric cleavage of conventional and aromatic carotenoids doc
... demonstrated that MtCCO mediates the symmetrical cleavage of b-carotene at the C15-C15¢ site, as well as the asymmetrical cleavage of the C13-C14 or the C13¢-C14¢ double bond.To test the cleavage ... source of apocarotenoids. In mam-mals, the synthesis of apocarotenoids, including retinoic acid, is initiatedby the b-carotene cleavage oxygenases I and II catalyzing either a centralor an excentric ... b-apo-8¢-(C30) and b-apo-10¢-carotenal (C27), as well as theirhydroxylated derivatives, 3-OH-b-apo-8¢- and 3-OH-b-apo-10¢-carotenal. Table 1 gives the Kmand kcatvaluesdetermined in the biphasic...
- 12
- 377
- 0
Báo cáo khoa học: Atrial natriuretic peptide-dependent photolabeling of a regulatory ATP-binding site on the natriuretic peptide receptor-A pptx
... cyclase by the atrial natriureticfactor. FEBS Lett 219, 375–379.21 Marala RB, Sitaramayya A & Sharma RK (1991)Dual regulation of atrial natriuretic factor-dependentguanylate cyclase activity ... Sharma RK (2002) Evolution of the membrane guany-late cyclase transduction system. Mol Cell Biochem 230,3–30.40 Yamazaki A, Yu H, Yamazaki M, Honkawa H, Matsu-ura I, Usukura J & Yamazaki ... preserve the phosphorylation state of the receptor. No125I-labeled ANPradioactive signal remained in the membrane preparation, as meas-ured using a gamma counter. Membrane preparations were thenused...
- 12
- 338
- 0
Báo cáo khoa học: The mystery of nonclassical protein secretion A current view on cargo proteins and potential export routes pdf
... secretedearly after monocyte activation and HMGB1 at a laterstage. IL-1b consistently acts at an early phase of inflam-mation whereas HMGB1 functions as a late mediator ofinflammation (see above). These ... proteins in mammalian cells revealed the existence of a machinery that is capable of translocating the protein across the plasma membrane [103], demonstrating a conserved pathway among lower and higher ... parallel quantitativemeasurements of the appearance of unrelated cytoplasmicproteins in cellular supernatants [8,9] as well as the identification of mutants that are deficient in nonclassicalexport...
- 11
- 507
- 0
Báo cáo khoa học: The competitor-introduced Gc recruitment system, a new approach for screening affinity-enhanced proteins doc
... that directly upstream of PHOP2(5¢-ATACAATTAATTGACATCAGCAGACAGCAAATGCACTTGATATACGCAGCTCGACTACGTCGTAAGGCCG-3¢ and 5¢ -ATCTTTCAAATAGAGCCTGG-3¢). The amplified DNA fragments were used to transformBFG2Z18-K3 5A, ... 5¢-AAATATAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGCGTAAAGGATGGGGAAAG-3 ¢. The final ratio of target cells was determined by the number ofcolonies retaining the target gene divided by that of total ... 5¢-TTTTGTCGACATGGCGCAACACGATGAAGCCGTAGACAAC-3¢ and 5¢-AAAAGGATCCTTATTTCGGCGCCTGAGCAT-3¢, and inserted into the SalI–BamHI sites of pGK425 [19], yielding plasmidspLMZ-WT and pLMZ-K3 5A, respectively. The...
- 9
- 356
- 0
Từ khóa: Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ