Báo cáo khoa học: Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs potx
Báo cáo khoa học: Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs potx
... Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs Keiichi Ishihara, Nobuyuki Yamagishi and Takumi HatayamaDepartment of Biochemistry, ... °Cfor 1 h and furt her incubated for 6 h , the cellular levels of Hsp105a and Hsc70 (a constitutive isoform of Hsp70)increased, and t he expression of Hsp70 (an inducibleisoform of Hsp70) was ... manufacturer’s instructions.ResultsInduction of hsp promoter activity, activation of HSF and accumulation of hsps by IND and NDGAWe first examined the effect of IND and NDGA on thehsp105 promoter (Fig....
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Báo cáo khoa học: Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1 pdf
... optimized by infecting Sf9 cells with different amounts of virus and by expression for various periods. Aliquots of the culture sup-ernatants and cell pellets were analyzed by SDS ⁄ PAGE and immunoblotting ... P212121 and diffracted X-rays toa resolution of 2.9 A˚(Fig. 4).Phl p 1 and Phl p 2 belong to different clusters of proteins as determined by phylogenetic analysisAmino acid sequences of seven ... Zea m 1 and Cyn d 1, a second consisting of Lol p 3, Dag g 3, Lol p 1, Phl p 1, Ory s 1 and Tri a 3 and a third cluster including Lol p 2, Hol l 1,Phl p 2 and Pha a 1. Although Phl p 1 and Phl...
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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx
... over-expres-sion of Hsp60 and Hsp70 and exhibit abnormal mor-phology in correlation with increased proliferation,premature differentiation and elongation, inhibition of denucleation and of loss of organelles ... (Tdag51) by direct HSF1 binding of a HSE in the proximal promoter region of the Tdag51gene. Tdag51 is a member of the PHL domain family and its N-terminal region is bound and inhibited by major ... normalconditions [62,63]. A genome-wide analysis of theregions bound by HSF2 and of transcriptome shouldsoon allow a broader understanding of the role of HSF2 in brain cortical development.Hsf2tm1Ijb⁄tm1Ijbadult...
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Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf
... discussion, and Prof. F. Dall’Olio (Department of Experimental Pathology, University of Bologna) for critical reading of the manuscript. This work was supported by grants from MIUR (COFIN2001) and from ... oligosaccharidesformed in BxPC3 cells (j in lower part, and A, B, and C of upper part)andT5ASclone(h in lower part, and A, B, and C of upper part)metabolically radiolabelled with [3H]Gal ... gift of pEFneo vector, Prof. R. Tenni (Department of Biochemistry, University of Pavia) for the help with radioisotopefacilities, Prof. M. Valli (Department of Biochemistry, University of Pavia)...
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Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx
... 5 and 20 lM of benomyl treatment (lanes 2 and 3); and 40 h after 5 and 20 lM of benomyl treatment (lanes 4 and 5).Fig. 9. Benomyl induced hyperphosphorylation of bcl2 and dissoci-ation of ... Cleavage of PARP by benomyl treatment. HeLa cells were treated with the indi-cated concentrations of drugs for 40 h and equal amounts of celllysates were resolved by SDS ⁄ PAGE followed by immunoblottingwith ... resultssuggest that the suppression of microtubule dynamics by benomyl was the cause for the loss of tension acrossthe kinetochores and activation of the checkpoint pro-teins and induction of apoptosis.ResultsBenomyl...
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Tài liệu Báo cáo khoa học: Mechanisms of obesity and related pathologies: Androgen deficiency and endothelial dysfunction may be the link between obesity and erectile dysfunction pptx
... 5767MINIREVIEWMechanisms of obesity and related pathologies: Androgendeficiency and endothelial dysfunction may be the linkbetween obesity and erectile dysfunctionAbdulmaged M. Traish1, Robert J. Feeley1 and Andre ... pathophysiologicalmechanisms of endothelial dysfunction and testosterone deficiency includepenile vascular insufficiency as a result of the loss of nitric oxide synthaseexpression and activity and the loss of tissue ... endothelial dysfunction and androgendeficiency, all of which contribute to the pathophysiol-ogy of ED. Approximately 25% of obese individualsexhibit MetS [2] and obesity, as manifested by anincreased...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... assigned as c¢38+5 and z¢Æ67+5,respectively.Sequence coverage of Rev provided by ions of typec¢ and z¢Æis indicated by solid and dashed lines, respec-tively, above and below the sequence ... Fragmentation scheme for production of ions of type c¢ and z¢Æ by reaction of a low-energy electron with a multiply protonatedpeptide.ETD-MS analysis of peptides and proteins N. D. Udeshi et al.6270 ... acetylation and ubiquitina-tion of Lys, and phosphorylation of Ser and Thr [16–18]. Combinations of these modifications are suggestedto constitute a ‘histone code’ that regulates the binding of protein...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... the protein bands were analyzed by calcula-tion of the percentages of the di- (d), mono- (j) and nonglycosy-lated (m) isoform as arithmetic means of separate gel runs. Thenumber of gel runs ... nonglycosylated isoform (m) of PrPCwere quantified and calcu-lated as percentages of the total signal. The glycoforms of the pro-tein bands were analyzed as arithmetic means of separate gelruns. ... 8G8 and P4 for sheep,SAF34 and P4 for cattle, and SAF34 for mice; and for the C-ter-minal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs6H4, SAF60, SAF70 and SAF84 for sheep, cattle and...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... highlighted in green and the nitrogen atoms of R120 and K22 in blue.The open form of MurA is unliganded (A) and the closed conformation (B) contains fosfo-mycin (orange) and UDPNAG (purple) ... UDP-N-acetylmuramic acid) (A) and inactivation mechanism of MurA by fosfomycin as a result of the covalent linkage between Cys115 of MurA and fosfomycin (B).Ó FEBS 2004 Lysine 22 and arginine 120 in MurA ... independent of the thermodynamic and kineticproperties of the binding process and is solely based on thedetectability of the peptides of interest. We have used thismethod to show that binding of fosfomycin...
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Tài liệu Báo cáo khoa học: "Order of Subject and Predicate in Scientific Russia" pptx
... twenty-eight pages of continuous text2 were analyzed for the relative positions of the subject and the predicate. The predicate spot was determined syntactically, by its func- tion, and the following ... " In support of this statement, Harper quotes certain figures: "From a sample of 1, 528 sentences containing a subject and verb: Subject before verb: 81% of all occurrences Verb ... four-fifths of the time." Proceeding from these assumptions, Harper builds his system of mechanical translation of Russian upon word-for-word translation, strip- ping the Russian words of their...
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