... made up of two f unctional domains,one corresponding to the cofactor-binding domain, orRossman fold (residues 1–147 and 313–334 in spinachGAPDH (accession code in PDB:1JN0), the other being thecatalytic ... directly involved in the interaction with thesmall protein. It is likely that the introduction of thenegative charge destabilizes the S loop, thus indicating thatthis loop, in addition to its role ... prote in [11,12]. On thecontrary, the interface of transitory complexes bears m anycharged residues, and its composition is closer to t hat of solvent-exposed regions of the p rotein. The arginine...