... primer, 5¢-GAGAGAGGATCCATATGACAAAAACCGAGTTAC-3¢, NdeIsite; antisense primer, 5¢-GAGAGAAAGCTTCAAAACTGGGACAGTTG-3¢, HindIII site. The same method was used to amplify the codingsequence for the E.coliMGST, ... purification,functional characterization, and projection structuredetermination. J Biol Chem 27 8, 22 199 22 209. 23 Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E,Nakamura Y, Miyajima N, Hirosawa M, Sugiura M,Sasamoto ... to the nucleo-tide sequence 10 655–11 080 in the E. coli genome with a GTG start. Sense primer, 5¢-GAGAGACATATGCCATCGGCCATTTTAAAG-3¢; antisense primer, 5¢ -GAGAGAAAGCTTCTAACGCAGGGAGAAAAC-3¢....