... performed in
30 mL of 2.5 m
M
of Tris/HCl buffer, pH 7.0 and 0.1
M
NaCl. Release of fatty acid was titrated with 0.1
M
NaOH.
Enzymatic activity was expressed in units (U) per mg of
protein where ... identity ranging from
44% to 31%. These structural homologs, which are
members of the human HSL protein family, include the
thermophilic esterase from Bacillus acidocaldarius [29...
... of fatty
acid predominating in the oil, but also contained a
complex mixture of other fatty acids, as occurs in vivo.
As might be expected, the fatty acid composition of
CRLPs containing TG from ... (SFA), fatty acids decreasing the risk
[22]. Our earlier work has shown that the fatty acid
composition of chylomicron remnants reflects that of
the diet [23], and modul...
... kinetics of
the various mutants.
The proposed model for transfer of the acyl chain
from ACP to KAS III, a CHN group enzyme, consists
of two steps: specific, albeit weak, binding of ACP to
the docking ... [25]. The formation of the fatty acid
complex is accompanied by the emergence of a well-
defined water molecule within hydrogen bond distance
of His333 N
e
(Fig. 3A,B).
Stru...
... of their target genes.
Indeed, several examples of feedback regulation that
involve miRNAs have been reported. Below, we attempt
to summarize our recent understanding of feedback
regulation of ... Culture of Japan (G.T.); the Program for
Promotion of Fundamental Studies in Health Sciences
of the National Institute of Biomedical Innovation
Table 1. miRNAs involved in the fe...
... of very long chain polyunsaturated omega-3
and omega-6 fatty acid in plants. Nat Biotech 22, 1–7.
12 Qiu X, Hong H & MacKenzie SL (2001) Identification
of a Delta 4 fatty acid desaturase from ... &
Mukerji P (2004) Identification of two novel microalgal
enzymes involved in the conversion of the x3 -fatty acid,
eicosapentaenoic acid, into docosahexaenoic acid. Bio-...
... level of
inhibition consistent with a physiological role (IC
50
of
120 lm), whereas the worst inhibitors tested were
the three acid analogues NAMN, NAAD and nicotinic
acid, with IC
50
values of ... C. Smith
2
and John M. Denu
1
1 Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI, USA
2 Department of Chemistry, University of Wisconsin, Madison, WI, U...
... mechanism of regulation of both domains involves a
common iron dependent process [74,75,79].
Regulation of HIFa subunits
by oxygen-dependent prolyl and asparaginyl
hydroxylation
A variety of oxygen ... of
regulation by oxygen, it has been the recent analysis of HIF-
1a and HIF-2a protein stability and transactivation potency
that have provided the greatest insights into oxyg...
... subsequent degra-
dation of the receptor in transfected human embryonic
kidney cells [50].
Direct regulation of the EGFR by CaM
The direct regulation of the EGFR upon binding of
the Ca
2+
⁄ CaM complex ... C-terminal lobe of the kinase domain of one of the
monomers forming the dimeric receptor interacts with
the N-terminal lobe of the apposed monomer
[19,47,84]. Of relev...
... binding of U2AF. The
lower panel shows that most of the HIV-1 3¢ss deviate from the
consensus because of their low content of pyrimidine nucleotides.
A
B
Fig. 4. Positive and negative regulation of ... the activation of site A3 (25% of the
overall activation). Therefore, the most important
parameter of site A3 activation is expected to be the
displacement of protein hnRN...