Báo cáo khoa học: Functional interaction of Escherichia coli heat-labile enterotoxin with blood group A-active glycoconjugates from differentiated HT29 cells docx
Báo cáo khoa học: Functional interaction of Escherichia coli heat-labile enterotoxin with blood group A-active glycoconjugates from differentiated HT29 cells docx
... recognized blood group A-active glycoconjugates on the cell sur-face of undifferentiated [16] and differentiated HT29 cells (Fig. 4). Although no direct quantification of blood group A-active glycoconjugates ... Binding of Escherichia coli heat-labile enterotoxin (LT-I) to HT29 cells in culture. Differentiated and control HT29- ATCC (A)and HT29- 5F7 (B) cells were incubated with increasing concentra-tions of 125I-labelled ... 0.4 HT29- 5F7 differentiated 2270 ± 120 1600 ± 130 2.1 ± 0.3ABFig. 6. Blood group A-active glycoconjugates from differentiated HT29 cells and their ability to interact with Escherichia coli...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... character-ization of E. coli Pta and set out to investigate thefunction of its N-terminal domain by the construc-tion and analysis of three E. coli Ptas with deletions from the N-terminal ... PCR from plasmidpCA24N–Pta, hereafter called pPta, containing the entirecoding sequence of pta from E. coli. Different sets of primerswere used to amplify different E. coli Pta fragments from the ... occu-pancy in Escherichia coli. Nat Chem Biol 5, 593–599.15 Kitagawa M, Ara T, Arifuzzaman M, Ioka-NakamichiT, Inamoto E, Toyonaga H & Mori H (2005) Completeset of ORF clones of Escherichia coli...
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Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
... thepattern of splicing of E1A mRNA in comparison with empty pEGFP vector (Fig. 4B). Expression of EGFP–Ki-1 ⁄ 57 leads to formation of the 10S and 9S iso-forms, concomitantly with a reduction of 13S ... malig-nant cells from Hodgkin’s lymphoma. Despite studies showing its phos-phorylation, arginine methylation, and interaction with several regulatoryproteins, the functional role of Ki-1 ⁄ 57 ... colocalized with theCajal body protein marker p80-coilin in the nucleus of HEK293 cells treated with Adox (Fig. 7Ai–iii). Thisfinding may, in addition, strengthen the hypothesis of the involvement of...
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Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx
... only treatment of CTPS with trypsin produced a limited number of cleavagefragments over the course of 1 h, of which the formation of some fragments was suppressed in the presence of ATP and/or ... and purificationWild-type and mutant forms of recombinant E. coli CTPSwere expressed in and purified from E. coli strainBL21(DE3) cells transformed with either mutant or wild-type plasmid pET15b-CTPS1 ... is consistent with previous reportsthat suggested interactions between the GAT and synthasedomains within the tertiary structure of the enzyme[17,28,55]. The existence of such interactions...
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Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... thefunction of the RC gene of Escherichia coli. Nature 221, 838–841.14. Cashel, M. & Kalbacher, B. (1970) The control of ribonucleic acidsynthesis in Escherichia coli. V. Characterization of a ... h)1in both groups of media.Therefore, the growth rate of GRd.tsf ranges from 70–95% of that of UY211 (Fig. 3). The lowest growth rate of GRd.tsf (70%), relative to the growth rate of UY211, ... min)1) from each exchange reaction wasplotted as function of the added amount of EF-Ts. Theslope of the GDP exchange in the absence of EF-Ts wassubtracted from each slope value. The exchange of...
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Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
... hydrogen-bonded with Pro216 and Tyr218 from the other subunit, and finallyleading to the disintegration of tetramers of the enzyme.This can be fitted well with the explanation of why theE59L ... Hung HC (2006)Determinants of the dual cofactor specificity andsubstrate cooperativity of the human mitochondrialNAD(P)+-dependent malic enzyme: functional roles of glutamine 362. J Biol Chem ... Regulatory properties of the enzyme from Ascaris suum. J Biol Chem 251, 3599–3602.23 Hung HC, Kuo MW, Chang GG & Liu GY (2005)Characterization of the functional role of allosteric siteAsp102...
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Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt
... Authors Journal compilation ª 2009 FEBSThe interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of itspeptidyl-prolyl isomerase activityLuigi ... activity of SlyD is not required formaturation of Escherichia coli hydrogenase. J Bacteriol189, 7942–7944.25 Leach MR, Sandal S, Sun H & Zamble DB (2005) Metalbinding activity of the Escherichia ... Structural traces of full-length SlyD.Fig. S2. Electrostatic surface potential for SlyD.Fig. S3. Interactions of SlyD with FK506 and rapamy-cin.Fig. S4. NMR titration experiments of SlyD with Ni(II)...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... con-sumption of O2and substrate with the formation of detectable products. Figure S4 shows that conversion of 2,4-pentanedione (5 mm) proceeded with depletion of a molar equivalent of dissolved ... orbital with the 3d orbital of the metal cofactor.Two important pieces of information can be gleaned from the pre-edge peak. First, occurrence of this tran-sition implies distortion of the metal ... comparison of nonheme metal-dependent enzymes.Fig. S2. Overlay of the homology model of Bxe_A2876(white) with the template crystal structure of RgCarb(RgCAR) (yellow) and the crystal structure of...
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Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx
... phos-photransferase system (PTS) in Escherichia coli is one of the best studied biochemical networks from theo-retical and experimental points of view, and hasKeywords Escherichia coli; model verification; ... model [14]Table 2. Comparison of the predictions of the specific glucoseuptake by the model of [15] with the model of [18], and with theexperimental results of [15].StrainNishioet al.[15]aNishioet ... taken from [23]; theoretical predic-tions from the model of Nishio et al. [15] (full line with squares);theoretical predictions from the model of Bettenbrock et al. [18](broken line with diamonds);...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... particular,phosphorylation of dGuo was reduced markedly.Structure of Dm-dNK-E52D in complex with dTTPThe structure of one of the mutants, E52D, was solvedusing X-ray crystallography at a resolution of 2.5 A˚incomplex ... a structuralstudy of Dm-dNK in which the enzyme was cocrystal-lized with both deoxythymidine (dThd) and dTTP sep-arately, E52 formed a hydrogen bond with the 5¢-OH group of dThd, whereas it ... quantitation of microgram quantities of protein util-izing the principle of protein–dye binding. Anal Biochem72, 248–254.22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B &Loffler M (1996) Functional...
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