... ions (Fe, Ni) and CO and CN ligands in the active centre, the orientation of the Fe–S clusters within the small subunit, and the proteolytic cleavage of the C-terminal end of the largesubunit by ... each other in the hupK gene and its regulatoryregion. One of them contained the hupK-hypC1DE genes(pBRKCDE), and the other one contained only the hypC1DE genes (pBRCDE). The presence of the ... be identified in the vicinity of the hynSL genes [16] and the structural genes of the soluble hydrogenase.Downstream from the hupSL structural genes, accessorygenes (hupDHI )andthehupR gene (corresponding...