... in the highest yield, and they all were
among the amino acids found in the Murchison mete-
orite [11]. Both the high content of the ‘very early’
amino acids in these sequence motifs, and the ... along the protein chain (Fig. 3).
From the resulting plot it is clear that most of the con-
served residues are located on the cytosolic side of the
J. Hedlund et...
... carbonyl
of Asn by the nitrogen of the peptide group linking the
Asn to the following residue [4–6]
2
. As hydrolysis of this
intermediate may occur on either side of the imide
nitrogen, the Asp ... containing the regulatory and catalytic domains [15].
Fig. 2. Stereo picture of the three Asn residues in the N-terminal autoregulatory sequence of rPAH. Figure b...
... novel
sequence motifs in the p26 N-terminal extension and
another in the C-terminus. The deletion of these
motifs, termed G (multiple glycine), R (multiple argin-
ine), and TS (multiple threonine ... one
enriched in glycine and the other in arginine. Addition-
ally, the C-terminus contains an unusual stretch of
residues endowed with threonine and serine. These
regio...
... mutant
proteins. This was interpreted as a lack of fosfomycin
binding to these mutant proteins [12]. Binding of fosfomycin
to either wild-type MurA or the K22 mutant proteins in the
absence of UDPNAG is not ... of R120 by lysine reduces the probability of
forming the fully closed form of the protein.
The results obtained with the K22V/R120K double
mutant protein po...
... by
the C domain of the initiation module of dapto-
mycin. That DptE and DptF are involved in the
lipidation process of daptomycin was first shown by
Miao et al. [1]. In their work, the daptomycin ... is GLDSV,
indicating that this putative ACP domain is one of the
few ACPs in which valine replaces isoleucine (I) or
leucine (L) in the conserved sequence. To determin...
... of the MR151-
derived strain with the crtIa deletion (MR841) com-
pletely lose the red color typical of colonies from the
parental strain, suggesting that the absence of CrtIa
blocks the synthesis ... compilation ª 2007 FEBS 4307
in- frame deletion of crtIa in M. xanthus strain MR151.
This strain contains a mutation, carR3, which renders
the expression of the carB...
... have been identified in those strains
that are responsible for most of the steps in the biosynthesis
of the oligosaccharide portions of the LPS molecules. The
inner core of H. in uenzae LPS has ... from the nasopharynx to the middle ear via the
eustachian tube, often in the presence of viral respiratory
infections [5]. Whether all colonizing strains of H....
... are the pH and E
h7
in the
experiments with the addition of inhibitor (the arrow indicates the
time of addition); curves (C) and (D) are the pH and E
h7
in the
control experiment.
Thiol groups in ... addition of NEM or AMdiS to a reducing
culture of L. lactis rapidly increased the E
h7
and
restored the initial E
h7
value (Fig. 3). These data show
the implicati...
... sequence of OPN and
the N-terminal sequence of dentin matrix acidic phos-
phoprotein 1 (DMP1) [27,28] and long sequences of
Glu residues in, for example, integrin-binding sialo-
phosphoprotein ... sequences found in
SPs with known involvement in mineralization are
shown in Table 1, and aligned sequences of their
orthologues are given in Doc. S2 (see Supporting infor-
m...
... determined
using 25 n
M
[
3
H]PonA and increasing amounts of proteins
in individual incubations. The percentage of specific b inding
increased in a protein concentration-dependent manner
within the range of ... fractions with
increasing concentrations of [
3
H]PonA, then subjecting the
binding data to Scatchard analysis (Fig. 4). The analysis
showed the presence of...