... the dyana algorithm. The outcome was a set of 20
structures with a mean global rmsd of 0.56 ± 0.16 A
˚
and a
mean global heavy atom rmsd of 1.30 ± 0.28 A
˚
.
Structural refinement was carried ... Solution structure of an M-1 conotoxin with a novel
disulfide linkage
Wei-Hong Du
1,2,
*, Yu-Hong Han
3,4,
*, Fei-juan Huang
1,
*, Juan Li
2
, Cheng-Wu Chi
3,4
a...
... ascomycete fungal laccase from
Thielavia arenaria – common structural features of
asco-laccases
Juha P. Kallio
1
, Chiara Gasparetti
2
, Martina Andberg
2
, Harry Boer
2
, Anu Koivula
2
, Kristiina Kruus
2
,
Juha ... Paloheimo M, Valtakari L, Puranen T, Kruus K, Kallio
J, Ma
¨
ntyla
¨
A, Fagerstro
¨
m R, Ojapalo P & Vehmaan-
pera
¨
J (2006) Novel laccase enzyme and use thereof.
Europ...
... have determined the structure of HtA by NMR methods. The
structure consists of one a- helix, a helical turn and seven b-strands that
form an N-terminal hairpin and an anti-parallel b-sheet, with ... were analyzed in a semi-automated iterative manner
by cyana 2.1 [38]. The NOE coordinates and intensities
used as input for automated analysis were generated auto-
matically by...
... that protein family.
In addition, we show that both its fold and potential
surface partially resemble the structural features of the
antimammalian scorpion a- neurotoxins and of the human
antibacterial ... distance data by dynamical simulated annealing from a
random array of atoms. Circumventing problems associated with
folding. FEBS Lett. 239, 129–136.
31. Laskowski, R .A. , Rullm...
... duplex at molar ratios of 1 : 1.2 and of
1 : 2.4.
Spectra analysis
NMR data were analysed and processed with the computer
programs
XWINNMR
,
AURELIA
[22], and
AUREMOL
[23]
(Bruker, Karlsruhe, Germany). ... rmsd
values given in Table 1. Analysis of the Ramachandran plot
shows that the dihedral angles / and w for the secondary
structure elements are all found in the most favored or th...
... physiologi-
cal substrate remains a mystery [17].
Structurally, RICH protein consists of three regions:
a glutamate- and aspartate-rich N-terminal domain, a
catalytic phosphodiesterase domain, and a C-terminal
isoprenylation ... catalytic domains of RICH
and CNPase share a pair of conserved sequence motifs
H-X-(T ⁄ S)-X (Fig. 1B) with three other groups of
enzymes: fungal...
... chain protons of Leu86 and protons of
amino acids Ala14, Val55 and Leu81 are observed, none of
which are seen for the wild-type protein. Analysis of the
backbone dihedral angles F and Y of mutant ... -type and mutan t form of HPr are visualized by
a comparison of t he co rrespondin g backbo ne dihedral angles. Values for the wild-type and the mutant protein are indicated by w...
... Both mastoparan and D-somatostatin
blocked refolding.
Fig. 6. NMR titrations of 2 mM mastoparan by human PDI-bb¢
protein and a plot of the magnitude of changes in mastoparan
proton chemical shifts ... NMR or X-ray crystallogra-
phy. The overall shape of full-length human PDI has
been investigated by small-angle X-ray scattering and
shown, at low resolution, to adopt a flat annular...
... Watson and Crick b ase
pair formation between antiparallel strands. The model iso
ADNA duplex is characterized by the s ame value of slide,
X-displacement and the helical parameters, as ADNA. On
the ... Sundaralingam
3
and Narayanarao Yathindra
1
1
Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India;
2
Department of Chemical
Sciences, TIFR...
... violations greater than 0.5 A
˚
and r.m.s.d.
for bond and angle deviations from ideality of less than
0.01 A
˚
and 5°, respec tively.
Structure calculations for mouse aMT
All structure calculations ... Solution structure of Cu
6
metallothionein from the fungus
Neurospora crassa
Paul A. Cobine
1
, Ryan T. McKay
2,
*, Klaus Zangger
2,
†, Charles T. Dameron
3
and Ian M. Armitage...