Báo cáo khoa học: Stage-speciﬁc activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx

... and of ﬁve in the Caenorhabditis elegans genome. ADAMTSmembers contain a signal peptide, a prodomain, ametalloprotease (MP) domain, a disintegrin (DI)domain, a variable number of thrombospondin ... results indicate that activation of MIG-17occurred in the basement membranes of the muscle,intestine and gonad in the L3 larvae.We next studied the activation of MIG-17 in adultanimals expressing ... staining of intestinaland muscular basement membranes was often faintand discontinuous (Fig. 5C). In the older adults,ABCDEFFig. 4. MIG-17 activation in L3 larvae. (A–D)Cross-sections of...

Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt

... B of cytokineinduced expression of intercellular adhesion molecule 1(ICAM-1) in an intestinal epithelial cell line, a processblocked by proteasome inhibitors. Gut 42, 779–787.23 Lakshminarayanan ... 4347indicating that LPS induces ICAM-1 expression in SCs, suggest that ICAM-1 may play a role in the focalaccumulation and antigen-induced activation of T cells in inﬂammatory demyelinating diseases of ... 134–141.Supporting informationThe following supporting information is available:Fig. S1. LPS induces the expression of ICAM-1mRNA in cultured Schwann cells.This supporting information can be found in...

Báo cáo khoa học: ATP allosteric activation of atrial natriuretic factor receptor guanylate cyclase pot

... domain, the site of ATP binding, exhibitssequence homology with the catalytic domains of tyro-sine kinases [30]; (b) staurosporine binds to the sameATP site as that of various protein kinases, ... bindingresults in 3D terms, the ARM domain model [30] wasanalyzed. The original 3D model of the ARM domainwas built using crystal structures of insulin receptorkinase and hematopoietic cell ... A˚.Docking of staurosporine to the ARM domainCo-crystallography studies have shown that stauro-sporine binds to the ATP binding pocket of proteinkinases and that such binding induces conformationalchanges...

Báo cáo khoa học: Speciﬁc cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot

... thechanges in the thermal unfolding of actin induced byinteraction of G-actin with actin-binding proteins [25–27], G–F transformation of actin, and stabilization of F-actin by phalloidin and Pianalogs ... conformationaltransitions in actin crystals involve so-called DNaseI-binding loop (D-loop) in subdomain 2 of the actinmolecule [7]. In tetramethylrhodamine-modiﬁed ADP-G-actin, the D-loop was found to be in an ... sta-bility of G-actin may depend on the conformation of the nucleotide-binding cleft. This suggestion is sup-ported by the studies on the effects of actin-bindingproteins on actin structure.Actin-binding...

Báo cáo khoa học: Speciﬁc degradation of H. pylori urease by a catalytic antibody light chain pptx

... was capable of digesting factorVIII molecule [7], suggesting a pathological role of thisantibody in vivo. The Bence-Jones proteins, which arefound in the urine of patients with certain diseases,particularly ... The light chain of ECL2B mAb was capable of cleaving a pep-tide (RSSHFPYSQYQFWKNFQTLK) derived fromCCR5, a chemokine receptor, which plays a crucialrole in HIV infection. In all of these catalytic ... chain (HpU-9-L) strongly interacted with the b-subunit, but onlyweakly with the a-subunit. In this study, we investi-gated the binding and catalytic features of HpU-9 mAb subunits against...

Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx

... roles of CaM in intracellu-lar signaling, there is significant interest in betterunderstanding the structural basis of its recognition of target proteins.CaM is a 148-amino-acid protein consisting ... environment of the cell, the network of CaM-dependent signalingpathways may play a role in the cellular regulation of protein processing. Excess production of iNOS in the absence of sufficient ... studies involvingexchange of EF hand pairs within CaM have been per-formed to study specific interactions of CaM domainswith target enzymes during binding and activation [4,5]. The present investigation,...

Báo cáo khoa học: The C-terminus of viral vascular endothelial growth factor-E partially blocks binding to VEGF receptor-1 docx

... the C-terminus also increased VEGFR-1 binding and migration of THP-1 monocytes indicating that both the C-terminal residues andO-linked sugars contribute to blocking viral VEGF binding to VEGFR-1.The ... in bioassaysthat detect receptor binding and cross-linking at the cell surface. These assays made use of BaF3 cell linesexpressing chimeric receptors consisting of the extracel-lular domain ... ORFVNZ2VEGF, wasgreater than the increase in VEGFR-1 bindingobserved in the receptor-binding assay (Fig. 4).Fig. 3. Deletion of the C-terminus of ORFVNZ2VEGF increases binding to VEGFR-1 and VEGFR-2....

Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx

... special type of ubiquitin-binding domain also found in NEMO. Therelation ⁄ relevance of A20 interaction with ABINproteins regarding the inhibitory effect of the ubiquinediting complex of A20, Itch, ... modiﬁedwith K63-linked multiubiquitin chains, mediating therecruitment of various ubiquitin-binding proteinsinvolved in TNF signaling, but also with K48-linkedubiquitin chains that prompt proteasomal ... subgroup of TNF ligands and involves activation of NIK-mediated stimulation of IKK1 and conversion of p100-containing NF-jB complexes into p52-containing NF-jB complexes by proteolyticprocessing of...

Báo cáo khoa học: Probing the access of protons to the K pathway in the Paracoccus denitriﬁcans cytochrome c oxidase pdf

... state of E278[22], of individual heme propionates [23,24] and of Y280 [25] was previously studied employing this tech-nique in combination with site-directed mutants andisotopic labeling.Here ... mutants of E78II in the Paracoccus cyto-chrome c oxidase are characterized in terms of cata-lytic and proton pumping activity, kinetics of heme a3reduction and electrochemically induced ... activity of the E78IIA mutant, thekinetics of reduction of this mutant were investigatedby stopped-ﬂow spectrophotometry by anaerobicallymixing a degassed sample of the mutant in the oxidizedstate...

Tài liệu Báo cáo khoa học: "Evaluating the Accuracy of an Unlexicalized Statistical Parser on the PARC DepBank" docx

... and of differing syntactic annotation of similar infor-mation, which lead to problems mapping betweenour GR output and the current DepBank. Finally,differences in the linguistic intuitions of ... nodes representing lemma-afﬁx-tags and arcs representing named grammati-cal relations. The encoding of this mapping withinthe grammar is similar to that of F-structure map-ping in LFG. However, ... extended in a variety of ways – for example, by pruning PoS tags but al-lowing multiple tag possibilities per word as in- put to the parser, by incorporating lexical subcate-gorization into parse...

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