Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... FEBS 2481
Catalytic activation of human glucokinase by substrate
binding – residue contacts involved in the binding of
D-glucose to the super-open form and conformational
transitions
Janne Molnes
1,2,3
, ... E256 and E290) and the S-domain
(residues T168 and K169) (Fig. 1B). To identify the
contact residues involved in the in...
... on the surface of the hTNF-a protein,
enabling easy access to catalytic antibodies. The acces-
sibility of the TNF-a molecule to ETNF-6-H to is one
of the most important factors in cleaving the ... chains
capable of the degradation of urease in Helicobact-
er pylori. The catalytic antibody light chain, UA15-L,
could suppress the number of bacteria i...
... & Penning TM (1996) Character-
ization of the substrate binding site in rat liver 3alpha-
hydroxysteroid ⁄ dihydrodiol dehydrogenase. The roles
of tryptophans in ligand binding and protein fluores-
cence. ... core in this family [ 3–6 ]. To iden-
tify the catalytic residues involved in the PGDS and
PGFS activity catalyzed by AKR1B1, we introduced
sit...
... in
catalysis and the specific involvement of the catalytic
residues in the enzymatic reaction. The two zinc ions
in the active metal center are thought to participate in
substrate binding by activating the ... the
deprotonation of the zinc-bound water to form the
nucleophilic hydroxide and less involved in substrate
binding; and (c) the...
... leading to autophosphoryla-
tion of tyrosine residues on the cytoplasmic domain of the
receptor, thereby creating docking sites for adaptor pro-
teins, such as Shc and G rb2. The latter protein ... again after returning to a low level. On the
other hand, the s ame phosphorylation of EGFR by PKC is
known to cause decreased EGFR tyrosine kinase activity,
its binding...
... receptor Toll and thereby initiates a cyto-
plasmic signaling pathway, resulting in the release of a
rel family transcription factor Dorsal from the inhibi-
tor protein Cactus to activate genes involved ... perform immune functions. This
mechanism leads to activation of phenoloxidase, which
oxidizes catechols, leading to the formation of toxic
quinones and melanin...
... characteristic
of the fasting state. The inclusion of fatty acids resulted in
increased activities of the a-1 or the a-2 isoforms of AMP-
activated protein kinase (AMPK), increased phosphoryla-
tion of acetyl-CoA ... mechanism. The finding that epinephrine totally blocked
the activation of AMPK by palmitate is of note and requires
further investigation of t...
... throughout
the brain, and is involved in the regulation of energy
balance by stimulating increased food intake. Pep-
tide YY (PYY) is produced by the gastrointestinal
tract, and, via NPY receptor binding, ... of natural substrates is poorly characterized.
Identifying substrates is a crucial step in gaining
insights into the precise functions of proteases and
thei...
... responses,
the significance of ERK signaling in terms of regulating
miRNA expression is of great interest.
To study the role of the ERK1 ⁄ 2 pathway in the
regulation of miRNA expression, we first determined
the ... ERK1 ⁄ 2 activation (Fig. 4B). We
also confirmed inhibition of protein synthesis by
monitoring expression of c-Fos protein, which is a
Fig. 2. Acti...
... respect to ATP and noncom-
petitive inhibitors with respect to Rib5P. These results
indicate that binding of the substrates is ordered with
initial binding of ATP followed by binding of Rib5P.
In ... ordered binding of the substrates with ATP
binding first and a random release of the products
[16].
Discussion
The kinetic analysis of the K197A and R...