Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate speciﬁcity by metal cations, and allosteric behavior of the peptidase pdf

... 27 1–2 80 ª 2007 The Authors Journal compilation ª 2007 FEBS Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate speciﬁcity by metal cations, and allosteric behavior ... proteinengineering of this enzyme. In the present study, the prolidase- coding gene, pepQ,was isolated from Lactococcus lactis NRRL B-1821 and cloned. Characterization of the recombinant proteinrevealed ... understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods.Results and DiscussionCloning and expression of Lc. lactis prolidase The...

Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identiﬁcation of residues essential for glucanosyltransferase activity and folding pot

... pathogens, the cell wall is involved in the interaction with the host cells and in virulence. The biogenesis of the extracellular matrix is a fascinating aspect of yeast m orphogenesis. The elucidation ... ollowed by the release of the cleaved product and stabilization of the carbon cation by the catalytic nucleophile. The new reducing end is thentransferred to the hydroxyl group at the 3 -position of ... the proteins was examined.Aliquots of medium containing the recombinant proteinswere treated with Endo H and analysed by Western blottingusing anti-Gas1p immunoglobulin (Fig. 2C). The apparentmolecular...

Báo cáo khoa học: Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) ppt

... up in the gel(Fig. 1B, lane 6). A subsequent incubation of the gel in DPI resulted in inhibition of the lower band only(Fig. 1B, lane 8). On the basis of the size of multimers of ferritin used ... oligomer of the small form. The different in uences of DPI on the two forms could be caused by its failure to access the ﬂavin cofactor in the large form of complex I,whereas the cofactor remains ... Duve Institute of Cellular Pathol-ogy) for critical reading of the manuscript. This workwas supported by grants from the Grant Agency of the Ministry of Education of the Slovak Republic and the...

Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle ppt

... fisocitrate, and indeed, the site-specific mutagenesis of K253 to Met led to an inactive protein [23]. In addition, the alkylation of the adjacent M258 inactivated the IDH from A. vinelandii [26]. These ... ere not identified in the 5¢-flanking region of Cl-idh. No open reading frames werefound in the immediate vicinity of the gene. The deduced amino-acid sequence of Cl-IDH was 66.0% and 57.4% identical ... its role in regulating the d irection and flux of the RTCA cycle. For further understanding of the R TCAcycle, we are carrying out detailed investigations of eachmember of the cycle. With respect...

Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf

... amino acids in the Triton-bound con-formation of SCP-2. The amino acids lining the cavit-ies in the E. lagascae and A. thaliana Tr-SCP-2 models and in the structure of the SCP-2-like domain of human ... mode of ergosterol in the structural model of A. thaliana SCP-2. The bindingmode in (A) has higher ﬁtness than the binding mode in (B). The polar amino acids at the opening betweenhelices D and ... half of the amino acids in the cavity are conserved, including the three polar amino acids at the second opening.Nevertheless, we could identify some interesting differ-ences in the two proteins...

Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

... [1]. The variety of identified substratesindicates a critical role for PS in cell metabolism involvingcontrolled cleavage of protein transmembrane domains and signal transduction. The role of ... trans-membrane domains six and seven to generate heterodimers of N-terminal fragments (NTF) and C-terminal fragments(CTF) [7]. Many binding partners for the PS proteins havebeen identified including substrates ... impaired in PS1-deficient cells, and down-regulation of NCT inhibitsc-secretase cleavage of APP and Notch [1 1–1 3]. PEN-2 isa small double membrane-spanning protein with N- and C-termini facing the...

Báo cáo khoa học: Characterization of thermostable aminoacylase from hyperthermophilic archaeon Pyrococcus horikoshii pot

... that, in PhoACY, the mutation of one zinc ligand allows the local loosening of Zn2+ and the intervention of another Zn2+ in the metal- binding pocket, resulting in the incorrect coordination of ... shown by a pink sphere.(B) Speculated zinc-binding domain of PhoACY. Zinc ion is shown by a pink sphere. The residues related to zinc binding are shown by the balls and sticks. Characterization of ... Differences in the substrate speciﬁcity between thermostable amino-acylases were found in spite of the high similarity in their amino acid sequences. The substrate speciﬁcity could be altered by the...

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... nonsteroidal agonistsbind exclusively to the EcR ligand-binding domain(LBD), although the presence of USP increasesligand-binding afﬁnity [1 2–1 5]. The diversity of ligand-responsive characteristicsseen ... stretch of 3 5–3 7 aminoacids that lie just to the N-terminal side of the DBD(Fig. 1C). The EcRA isoforms from the two speciesshare a few similar motifs in the middle region of the A ⁄ B domain (Fig. ... beginning at the ﬁrst amino acidfollowing the LdUSP DBD. Both amplicons and the pVP16vector were digested with EcoRI and HindIII restrictionendonucleases. Ligation of the products into the linearizedpVP16...

Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot

... that the OSs from the wild-type strain consisted of terminal-, 2-, 4-, and 3,4,6-linked glucosyl residues, terminal- and 4-linkedgalactosyl residues, and a terminal-linked N-acetylglu-cosaminosyl ... deprotonated [M-H] – ions. The source of the ions was as indicated in the structure shown in the inset. The m ⁄ z 917.4 and 797.4 are due to sodiated and dehydratedforms, respectively, of the m ⁄ z 895.4 ... MALDI-TOF-MS of de-O-acylated LOS from the mutant and glycosyl composition, linkage, and NMR analysis of its OS indicated that the LOS contained a truncated core OS and consisted of a Glc-Kdo2(linker)-lipid...

Báo cáo khoa học: Activation of crystalline cellulose to cellulose IIII results in efﬁcient hydrolysis by cellobiohydrolase pot

... cellulose-binding domain (CBD) [ 7–1 0]. As the ini-tial step of the reaction, they are adsorbed on the surface of crystalline cellulose via the CBD, then glu-cosidic linkages are hydrolyzed by the ... adsorption of Cel7A after incubation for 120 minas representative results of four time points (120, 180, 240, and 320 min). The lines indicate the ﬁtting of the data to the two-bind-ing-site ... that the speciﬁc activity of adsorbed enzyme (k) is greatly in uenced by the crys-talline polymorphic form of the substrate. Moreover, in the cases of cellulose Ib from Halocynthia and hydrothermally...

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