... into two exons (IA and IB) in the
X. laevis gene, with the site of the intron insertion localized
within the 5¢ UTR region of t he xMGP gene (Fig. 1 and
Table 2 ). The other introns (2, 3 and 4) ... [23]. The construction of the )70/)36TATA
LUC and 3x( )70/)36)TATALUC involved t he cloning of
one or three copies of double stranded oligonucleotides
spanning...
... sequence of
the L. australasiae ecdysone receptor was similar to that of the ecdysone
receptor- A of the soft tick, Ornithodoros moubata (68%) and to that of the
ecdysone receptor- A1 of the lone ... the ecdysone receptor and the retinoid X receptor were cloned
from the Japanese scorpion Liocheles australasiae, and the amino acid...
... and one oxygen
atom of the side chain carboxylic group of Glu668 and
between the carbonyl carbon atom of Pro of the substrate
Ala-Pro-pNA and the Ser630 oxygen atom of the enzyme to
hold the substrate ... affect the
type of inhibition and the K
i
value of the oligopeptides
Tat(1–9) and Trp2-Tat(1–9). On the other hand, corres-
ponding to the fourf...
... receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMP
ligand. Therefore, the binding of the receptors of both subtypes is blocked and BMP ... the membrane. The binding domains of the receptor
chains have no contact with each other. They are bound
together solely by the BMP ligand. The BMP re...
... that the major T-cell antigens of
the Mycobacterium tuberculosis complex ESAT-6 and
CFP-10 form a tight, 1:1 complex and characterization
of the structural properties of ESAT-6, CFP-10, and
the ... constant (K
d
) of 4.8 · 10
)7
M. The thermal
unfolding of the heterodimer was completely reversible, with a T
m
of
48 °C. The comparative thermodynamics and therm...
... A
˚
) to the carbonyl oxygen of
Asp25¢, while the other conformation had the Ce atom at
5.51 A
˚
from the carbonyl oxygen of Asp25¢. In comparison,
the Leu90 and Leu90¢ in the PR showed the closest ... atom of indinavir formed hydrogen
bond interactions with the amide and OD2 of Asp29¢ ,and
interacted via water OB with the carbonyl oxygen of
Gly27, the OD1...
... structures of EcR and
USP from L. decemlineata (b) the analysis of mRNA
expression profile of L. decemlineata, EcR and USP,
and (c) the measurement of the binding affinity of
steroidal and nonsteroidal ecdysone ... dipteran, the yellow fever
mosquitoe Aedes aegypti [34], and lepidopterans, the
tobacco hornworm Manduca sexta [35–37] and the
silkworm Bombyx m...
... fitted to: G ¼ G
max
/
(1 + exp[–zÆ(V-V
½
)/25]), where G
max
is the maximum
conductance, z is the valence of the process and V
½
is the
midpoint voltage of activation.
Ionic current expression levels ... SCN1B .The
SCN1B gene spans 9kbonchromosome19
across six exons. Exon 3A is an extended exon
3(retentionofpartofintron3)viaalternative
splicing. Exons 1, 2, 3, 4 and 5 (soli...
... possessing
six conserved cysteine residues at the same positions as
that of other CHHs of penaeid shrimps (Fig. 1B).
Tissue-specific gene expression of L. schmitti
CHH gene
The size and expression of the ... osmoregulation
[10,11] and lipid metabolism [12] in different species.
Although the X- organ–sinus gland complex is con-
sidered the main source of CHH production,...
... (1982) Actions of insect toxins and other
toxins derivated from the venom of the Scorpion Androctonus
australis on isolated giant axons of the cockroach (Periplameta
americana). J. Exp. Biol. 97, ... similar to that of other scorpion
sodium toxins, but so far it is the longest among known
scorpion toxin introns.
Construction of expression vectors and expression...