... be involved in governing the activationmechanism of fumarate in the human and A. suumm-NAD-ME [27,32]. In our previous work, we delineated the functional role of Asp102, which is close to the ... Fumarate-binding site for human m-NAD-ME. (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3). (B) Fumarate-binding site of m-NAD-ME. The corresponding amino acids in the fumarate-binding ... charge of the residue and the allosteric property of the enzyme being retained, the kinetic properties are quitedifferent from those of the wild-type. The KA,FumandaKA,Fumvalues of the E59D...