Báo cáo khoa học: Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones potx

... (2009) 114 0–1 152 ª 2009 The Authors Journal compilation ª 2009 FEBS Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones Yvonne ... detailedview of the alignment is given in the bottom panels of Fig. 4 for the two hydrophobic pockets. Binding of Trp183 (dark blue) in hp1 induces a slight shift of helix a2 (blue), similar to the effect ... throughout the GABARAP spectrum and the disappearance of certain peaks (Fig. 2A). Again, the mutation of Trp183 to alanine in CRT(17 8–1 88) had atremendous effect on the binding properties of the molecule....

Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC?We have previously discussed the variation in the freeenergy of binding as a function of the changes ... movement of the molecule). Furthermore, the internal motions of each bond vector are independent of each other, but the rotational diffusion of the molecule affects each of thosebond vectors identically ... (within the range 0. 0–0 .3)[36,3 9–4 1].Next, we decided to use the model-free formalism[42,43] to obtain further insight into the apparentinternal mobility of the protein. However, the overalltumbling...

Báo cáo khóa học: Structural properties of the protein SV-IV potx

... both chromatograms refer to the protein segments 6 0–6 3,3 3–3 9, 6 4–7 8, 6 4–7 9, 8 4–9 0, 4 0–5 6, 5–3 2 + 7–3 2, 5–3 9 + 7–3 9. Peaks present in chromatogram A but not in B refer to segment 8 0–8 3. Peakspresent ... both forms. The CD spectra of the 1–7 0 and 7 0–9 0 regions of the proteinare reported in the inset of Fig. 1. The secondary-structureanalysis program estimated about 100% a-helix for the 7 0–9 0 ... and the equilibrium of self-association appears to control the biological properties of the protein [7]. Moreover, the immunomodulatoryactivity is related to the structural integrity of the...

Báo cáo khoa học: Structural ﬂexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for speciﬁc substrate recognition pptx

... different substrates and the cata-lytic domain of the apo-enzyme. The binding of the small substratesin the active site leads to conformational changes in the motif 2loop and SOL. The serine ... and the carbonyl oxygen of the serine substrate [12]. Thesemovements are required to position the carboxylateoxygen for nucleophilic attack of the a-phosphate of ATP. Thus, according to the ... interactions with the tRNA accep-tor stem. The model used to predict the amino acidsthat might participate in the binding of the 3¢-end of tRNA, which were therefore subjected to mutationaland...

Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

... the secondary structure of the catalytic domain of PKCfand the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presenceand the absence of MgATP. We used 2D ... structure of 282 residues of a total of 344have been solved [19]. The catalytic domain of PKChpossesses a high degree of homology ( 80%) with the catalytic domain of other isoforms of PKC belonging to ... increase). It seems,then, that this substrate was able to modulate the sec-ondary structure signiﬁcantly. To gain further insight into the structure and folding of cat-f and into the structural changes...

Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... importantclue for understanding the Nogo-40–NgR interaction.Recently, the deter mination of t he crystallographic s truc-ture of the N gR ectodomain l ed to the speculation thatone potential Nogo-66 binding ... sufﬁcient to stabilize a full helical conformation for the p eptide.NMR s pectroscopy was further utilized to explore the structural properties of Nogo-40. The very narrow reson-ance dispersion of ... induced to form a helical structure via interaction w ith Zn2+.Furthermore, the d etermination of the Nogo-40 solutionstructure offers a starting point for further understanding the interaction...

Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... fimbriae or other appendages of the bacterium,a hypothesis requiring further investigation.It is of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS ... to C6, 30 3–4 2, loss of ketene)confirmed that deuterium labeling was present. The chro-matographically isolated 2-amino-2,6-dideoxygalactosefraction was a mixture of the D-andL-forms of the aminoglycose, ... and2-amino-2,6-dideoxygalactose, the 1H-NMR spectra of which were identical with those of authentic referenceglycoses, and further confirms their characterization. The combined MS data and the isolation of the two...

Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx

... the association of the helices in the core of the oligomer, we calculated the electrostatic potential of the surfaces of the twolong helical segments predicted from the above algo-rithms; these ... 38 8–3 97 ª 2005 The Authors Journal compilation ª 2005 FEBS to be helical in nature, implicating that these could be the main contributors to the core of the oligomer. I697located in one of the ... stretch at the N-terminal has only some smallpropensity for formation of short helices, transiently,and the chain as such is highly dynamic.Structure of the core of the oligomersBecause of the large...

Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

... used for general assignments of the CPS andLPS O-chain polysaccharide: COSY, TOCSY, NOESY andHSQC. Due to overlap in proton resonances, selectiveTOCSY and TOCSY–TOCSY experiments were used to complete ... 3 of residue b (Fig. 3B).Proton resonances for the CPS sample did not appear asresolved multiplets due to heterogeneity resulting from the presence of both the amide a nd nonamide forms of GalNAcA ... wasused in most of 1D analogues of 2D NMR experiments. The 1D1H-NMR spectrum of CPS showed three reso-nances in the low-ﬁeld region ( 5. 3–4 .4 p.p.m.) in the relativeratio of 1 : 1 : 3, of which...

Báo cáo khoa học: Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis pdf

... essential for the thermal stability a ndbiological functions of the bacteria i n extreme environments[ 9–1 1]. The polar lipids found in Thermus aquaticus,Thermus ﬁliformis, Thermus scotoductus,andThermus ... study is the ﬁrst to determine the full structure of glycoglycerolipidin thermophilic bacteria Meiothermus spp. The structural information will be very useful for further investigations of the mechanisms ... glycerol diethers of the ther-mophilic anaerobe Thermodesulfotobacterium commune. Syst.Appl. Microbiol. 4, 1–1 7.6. Huber, R. & Stetter, K O. (1992) The order Thermotogales.In The Prokaryotes.(Balows,A.,Tru¨per,...

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