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Báo cáo khoa học: Functional role of the linker region in purified human P-glycoprotein pot

Báo cáo khoa học: Functional role of the linker region in purified human P-glycoprotein pot

Báo cáo khoa học: Functional role of the linker region in purified human P-glycoprotein pot

... This kinetic analysis provided new insightinto the role of the linker region. In addition, we fur-ther analyzed the functional role of the linker region based on the crystal structures of a ... cleavage of the linker [22]. Althoughthese results provided valuable suggestions for the role of the P-gp linker region, the molecular details, suchas the involvement of the linker region in substraterecognition, ... (2006) RNF2 interacts with the linker region of the human P-glycoprotein. Int J Oncol 29,1413–1419.26 Georges E (2007) The P-glycoprotein (ABCB1) linker domain encodes high-affinity binding sequences...
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Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

... preventing binding of oxygen.We analyzed the azide inhibition and determined the inhibition constants for sodium azide, in order to see the effect of the mutation (L559A) on the bindingproperties ... stability of the trinuclear site isdecreased, the binding of azide to the trinuclear sitemay be decreased. The depletion of the T2 copper andchloride ion may also affect the azide binding. In fact,it ... lastamino acids in the C-terminus of MaL. Deletion of the four last amino acids strongly reduced the expression of the protein in T. reesei and S. cerevisiae. Further-more, the deletion resulted in...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2–junctioncomplex. In this study the ... [14,20]. Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure ... important functional role, such as the correctpositioning of the SAP motifs in relation to the rest of the protein as well as for the stability of the protein. The truncated Ydc2 mutant proteins localizeto...
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Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the fibrin polymerization process pdf

Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the fibrin polymerization process pdf

... situated in the E-domain of the fibrinmolecule, the complementary site ‘c’ is suggested to besituated in the D-domain. We have tried to model the intermolecular binding between the D-domain of one ... the N-terminal region of the Aa-chain by the splitting off of fibrinopeptide A[6]. Site ‘a’ is formed by amino acid residues cGln329,cAsp330, cHis340 and cAsp364, situated in the cC-domain of ... situated in the bC-domain in a ‘knob’–‘hole’ type of interaction [7,9]. The remain-ing part of site ‘C’ (Bb19–46) probably remains boundto the fibrin D-domain. To determine the localization of a complementary...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... to DEC1 in the region between aminoacids 48–124 of DEC1. This region, including the bHLHdomain, coincided w ith t he r egion required for bo th the suppressive activity of DEC1 and the interaction ... Arg65, is responsible for t heE-box binding.Determination of the region in BMAL1 for bindingto DEC1To identify the region in BMAL1 required for the binding toDEC1, w e constructed e xpression ... eletion of 111 amino acids f rom the N-terminal region (BMAL1:112–626) did not affect the binding of BMAL1 to DEC1; deletion of 235 amino acids(BMAL1:236–626) slightly diminished the binding,although...
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Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx

Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx

... obtained by other groupswho also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutationalanalysis [35] and structural analysis of the plant defensinRs-AFP1 ... moleculecomprising Ôresidues in the protruding domain consisting of the type VI b-turn and the first part of b-strand 3Õ (i.e. the b-hairpin loop and part of the adjoining b-sheet) andÔresidues in the loop ... is not possible to infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general value in the mechanism of action of defensins. However, our...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... thisprotease at pH9 [10]. Further interest in the aromaticstacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing the functionally catalytic quadruple ... position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain atresidue 169 (Table 1 and Fig. 3A). However, the ASAs of Asp113 ... size of the side-chain at residue 169 and the pK2showed a clear linear relationship (Fig. 3B). The pK2increased as the size of the residue at position 169 increased. The effect of the size of...
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Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx

Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx

... Pex15p.To investigate the in uence of individual peroxins of the importomer on the composition of the membrane-bound AAA complex, we selected gene deletion strainsaffected in the docking complex ... acontrol. The corresponding solubilisates(right panel) were analyzed for the presence of Pex1p and Pex6p as indicated. The defici-ency of either protein of the AAA peroxincouple in the absence of ... 3805by the fact that the presence of transmembranesegments often interferes with the performance of integral membrane proteins in two-hybrid assays.Interestingly, Pex1p clearly interacted with the...
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Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx

Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx

... hydrolysis, resulting in anincrease in intracellular sterol trafficking. It is unclear, then,whether the corresponding increase in pregnenolone pro-duction results from an increase in free sterol ... ([14,15]). In the latter case, the concentration of Adrp limits the production of pregneno-lone [15] through a mechanism involving oxidized Adxp,that is in excess in the human placenta [16]. Whether ... (5¢-cagtagagacatgggagatcccccgcggaattcgagctcggtacccgggTCATTCTTTATTTAGAGCATCCAGC-3¢). The sequences in lower-case are complementary to the end of the GAL10/CYC1 promoter (lip1) and to the beginning of the PGK1 terminator (lip2). The 1647 bp PCRfragment was transformed into...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... anal-ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the twomodes of aglycone binding. The amino acid sequence of Zm-p60.1 was com-pared with the ... distances of reactingatoms. The only noted difference was in the orienta-tion of the aromatic ring of pNPGlc in the F193Amutant (Fig. 6D), owing to lost van der Waals contactwith the side-chain of ... modeling to obtaininsights into the molecular interactions underlying the observed changes in the mutants’ enzymatic kinetics.Modeling was only applicable to F193A because inter-pretation of...
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