Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx

... 4625MINIREVIEW Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes Ann-Kristin Mossberg1, Kenneth Hun Mok1,2, Ludmilla A. Morozova-Roche3 and Catharina ... 2010)doi:10.1111/j.1742-4658.2010.07890.x Human a-lactalbumin made lethal to tumor cells (HAMLET) and equinelysozyme with oleic acid (ELOA) are complexes consisting of protein and fatty acid that exhibit cytotoxic activities, ... HAMLET.Cytotoxicity of ELOA complexes Similar to HAMLET, the assembly of equine lysozyme and oleic acid into ELOA complexes led to cytotoxicactivity. ELOA effectively reduced the viability of mouse...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... withbinding of both MOF and Tip60 (equivalent to Esa1in yeast NuA4) [124,130]. However, in human cells depletion of MOF, but not Tip60, results in reducedglobal levels of AcH4-K16 and defective ... histone acetylation and deacetyla-tion, and transient recruitment of remodellers and transcription factors.A cycle of transcription commences with the recruit-ment of transcription factors and ... such as SAGA and NuA3, which mainly acety-late histone H3, and NuA4 which acetylates histone H4on K5, K8 and K12. This cascade of events leads to recruitment of transcription factor II H (TFIIH)...

Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx

... form of cyprosin was processedin different isoforms by the excision of the prosegment and of most of the PSI [21]. Conversely to what has been foundin vivo [31], heavy and light chains of the ... Biochemical and immunocytochemistry analysis of barley roots and leaves showed that phytepsin waslocalized to the vacuoles of these cells [80] and, in a differentstudy, phytepsin was also shown to accumulate ... also bythe 13 residues of the N-terminal of the mature enzyme and by the ÔﬂapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionicinteractions...

Báo cáo khoa học: Structure and function of KH domains docx

... which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja-cent and antiparallel to the b1-strand (Fig. 1). Thelength and sequence of the variable ... a1-helix and a2-helix and the b2-strand of KH1. Intimate contactsbetween protein and RNA continue across the cusp of the KH1 and KH2 domains. C46 binds to theFig. 6. Crystal structures of the ... composed of six antiparallel strands. This arrangement of KH molecules buries2100 A˚2 of total buried surface area with cross-strand side-chaininteractions.R. Valverde et al. Structure and function...

Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identiﬁcation of two active site cysteines pptx

... characterization of GlcNAc kinase and cysteine mutantsIn order to get a more detailed insight into the functions of speciﬁc cysteine residues of GlcNAc kinase, the Kmvaluesfor both substrates, GlcNAc and ... chloramphenicol and ampi-cillin. Cells were grown to an absorbance of 0.5, induced with1mMisopropylthio-a-galactoside for 2 h, harvested and resuspended in 20 mMNa2HPO4, pH 7.5, and then lysed ... For determination of the Km of GlcNAc, differentconcentrations of GlcNAc were used in the presence of 10 mMATP. For determination of the Km of ATP, differentconcentrations of ATP were used...

Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf

... (rmsd of 1.6 A˚for 1192 Ca atoms of a tetramer and 1.44 for 306 Ca atoms of a monomer).This indicates that not only is the monomer structure conserved, but also the quaternary structure of thetetramer. ... RevMicrobiol 50, 553–590.3 Ornston LN (1966) The conversion of catechol and protocatechuate to b-ketoadipate by Pseudomonas put-ida. II. Enzymes of the protocatechuate pathway. J BiolChem ... Nonetheless, both the tertiary and quaternary structures, and the structure of the active site, are similar to those of Pseudomonas putida3-carboxy-cis,cis-muconate lactonizing enzyme. One principal...

Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

... Sciences, Turku, Finland).Estimation of expression of cytosolic regulatoryproteinsTotal cell lysates of transfected CHO cells were used to esti-mate expression of Noxo1b, Noxo1c, and Noxo1a. Thelysates ... the following human celllines; androgen-independent prostate cancer LNCaP cells, androgen-independent prostate cancer PC3 and DU145 cells, and testicular germ cell tumor NEC8 cells [38,39].Splicing-speciﬁc ... Research and National Project on Protein Structural and FunctionalAnalyses from the Ministry of Education, Culture,Sports, Science and Technology of Japan, and CREST and BIRD projects of JST...

Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx

... expres-sion of the raptor or rictor genes (partners of mTORC1 and mTORC2 respectively) has only a slighteffect on the translation efﬁciency of TOP mRNAs.This result implies that mTOR regulates TOP ... sclerosis complex (TSC)1–TSC2, and the mammalian target of rapamycin(mTOR) C1 complex (composed of raptor, mLst8, and mTOR). The role of TSC1–TSC2 in the translation of TOP mRNAs has been recently ... steps of ribosome biogenesis and ⁄ or ribosome function. Indeed, a systematic study of the incorporation of RPs into preribosomes led to the identiﬁcation of the in vivo assembly pathway of the...

Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas ﬂuorescens potx

... a-helices. Residues involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg2+) and of Dom-a¢ of a neighbouring subunit (pyrimidinemoiety). ... Superposition of the ThDP cofactors of BAL (grey), ALS (cyan), AHAS (green), IPDC (purple), BFD (yellow), CEAS (blue), POX (pink) and PDC (red) together with some residues important for cofactor binding and ... known to play an important role in catalysis [25].The structures of all group members show ThDP inthe V-conformation that brings the C2 atom of thiazo-lium in close proximity to the N4¢ atom of...

Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx

... shifts for DMT1-TM4 in TFE and SDS.Ó FEBS 2004 Structure and topology of TM4 of DMT1 (Eur. J. Biochem. 271) 1951 Structure and topology of the transmembrane domain 4 of thedivalent metal transporter ... binding of peptides to membranes [37,45,46]. The exchange rate of the amide protons of DMT1-TM4 in both TFE and SDSmicelles was monitored by both 1D and 2D1HNMRspectroscopy (e.g. TOCSY and NOESY) ... from190 to 260 nm at a scan rate of 50 nmÆmin)1with a respondtime of 0.25 s, step resolution of 0.1 nm and band width of 1 nm. Each spectrum was obtained from the average of fourscans. Prior to...

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