Báo cáo khoa học: Optimization of conditions for the glycosyltransferase activity of penicillin-binding protein 1a from Thermotoga maritima ppt

... Optimization of conditions for the glycosyltransferase activity of penicillin-binding protein 1a from Thermotoga maritima Julien Offant1,2,3, Mohammed Terrak4, ... exemplified by a study of the fullectodomain of PBP1a from Thermotoga maritima (Tm -1a* ), extending our previous work on the GTasedomain from this protein (Tm-GT1a*) [38]. T. maritima is a Gram-negative ... investigate the substrate speci-ficity of the TPase reaction of Tm -1a* .Fig. 3. Screening of conditions for GTase activity of CYMAL-4-purified Tm -1a* .Tm -1a* (250 nM) was incubated in the presence of...

Báo cáo khoa học: "A Formula Finder for the Automatic Synthesis of Translation Algorithms" docx

... specifies the truth values of the variables in Dr for the given values of i and j. The program then evaluates the truth value of Dr in terms of the truth values of the component propositions. ... translated of the oscilla- tions of the voltage of the trigger . . . rather than by the smoother inverted construction . . . of the oscilla- tions of trigger voltage .In any event, the transla- ... all instances of the transformation, the formula finder ascertains whether the given variables can be combined into a logical formula that implies the given transformation. The formula finder...

Báo cáo khoa học: A kinetic model for the burst phase of processive cellulases pptx

... phase often utilizes the intersection p of the ordinate and the extrapolation of the steady-state condition for P1(t) (dotted line inFig. 1A) . This value is used as a measure of the amount of ... supports the cur-rent explanation of the double exponential slowdown.In the last section, we show two examples of how the analysis of the kinetic parameters may elucidatecertain aspects of the activity ... P1produced during the burst, i.e. the excess of P1with respect to the steady-state production rate, and it istherefore a measure of the magnitude of the burst. Anexpression for p is readily...

Báo cáo khoa học: A structural basis for the pH-dependence of coﬁlin F-actin interactions potx

... others [31], and the thrustingforward of subdomain 2 with respect to the rest of the monomer.In this report we study the pH-dependence of the actin–cofilin interface and provide evidence for ... controlledby the interplay of a variety of signaling pathways. The importance of the contribution of ADF/cofilins to cellmotility is reflected in their being regulated by many of thesesignals, ... to the sample(data not shown).Effect of pH on cofilin conformation The regulation of the cofilin activity by pH occurs in a pHrange suggesting the involvement of histidine residues. Infact, the...

Tài liệu Báo cáo khoa học: Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc docx

... vitamin D from the bloodstream for further metabolism [4].In conclusion, the identiﬁcation of new metabolites of vitamin D3 made by the action of P450scc willenable us to proceed with further biological ... arose by the action of P450scc, as well as the products that they gave rise to.This revealed both the major and minor pathways for the metabolism of vitamin D3 by P450scc.ResultsMetabolism of vitamin ... hydroxylate the D-ring of both secosteroids at C17. The conﬁgu-ration of the newly introduced hydroxyl group wasassigned as 17a based on the preferential hydroxyl-ation by P450scc from the less...

Tài liệu Báo cáo khoa học: "Generalized Expectation Criteria for Semi-Supervised Learning of Conditional Random Fields" pdf

... distribu-tions from majority label information: we assign .99 probabil-ity to the majority label of the feature and divide the remainingprobability uniformly among the remainder of the labels.Labeled ... andCRR07 use only the majority label of the word), itcan, however, be integrated into the GE criteria byusing the distribution from the relative proportions of labels rather than a the previous ... from a large corpus, andthen uses these features to augment the feature space of the labeled data. Since this is an orthogonalmethod for improving accuracy it can be combinedwith many of the...

Tài liệu Báo cáo khoa học: "Incorporating Context Information for the Extraction of Terms" pdf

... incorporating information gained from the textual context of the candidate term. 2 Context information for terms The idea of incorporating context information for term extraction came from that ... otherwise (2) a is the examined string, lal the length of a in terms of number of words, f(a) the frequency of a in the corpus, Ta the set of candidate terms that contain a, P(T~) the ... type from extended word units in that they cannot be freely modified" (Sager, 1978). Therefore, information from the modifiers of the candidate strings could be used in the pro- cedure of...

Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx

... of YidC had no major effect on the insertion of MscL, but formation of the pentamer was almostcompletely abolished under these conditions, suggest-ing a novel role for YidC in assembly of the ... equal to that of the non-depleted IMVs. Thisindicates that YidC is required for assembly of the MscL complex (Fig. 5).To investigate the role of the Sec translocon in for- mation of the MscL–HA ... bydepletion of YidC. The single-cysteine mutants of MscL wereexpressed from the pEH3 vector in the SRP depletion strain FTL10in the presence or absence of L-arabinose to control the expression of yidC....

Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx

... that the clearance and further metabo-lism of the allergen was altered as a result of the inflammation in the lungs of sensitized animals.Up to now there are few data available on the fate of ... At the earlier time points the lung showed the strongest radio- activity labelling. The radioactivity decreased with timeand at 48 h the radioactivity detected in lung wasapproximately of the ... Except for the 30 kDa protein in lymph nodes, they all correspond to mole-cular masses of easily identified known selenoproteins.There are 25 mammalian selenoproteins identified [20]. The two...

Báo cáo khoa học: "Data point selection for cross-language adaptation of dependency parsers" pot

... aconstituent-based parser on the training section of the Danish treebank and evaluated it on sentences of up to 40 words in the test section of the Swedishtreebank and obtained an F1-score of 66.40%. Dan-ish ... Figure 1, we use the remaining three tree-banks as training material for each language. The test section of the language in question is used for testing, while the POS sequences in the target train-ing ... marginal. Weobtain the best results for Arabic training using la-beled data from the Bulgarian treebank, and the bestresults for Bulgarian training on Portuguese only. The best results for Danish were,...

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