Báo cáo khoa học: High-resolution crystal structures of the flavoprotein NrdI in oxidized and reduced states – an unusual flavodoxin pot
Báo cáo khoa học: High-resolution crystal structures of the flavoprotein NrdI in oxidized and reduced states – an unusual flavodoxin pot
... tothese as the 40s and 70s loops, respectively, in baNrdI. The flavin moiety is sandwiched between Trp74 on oneface and the side chain of Thr42 and the main chainatoms of residues 4 2–4 4 on the ... P-loop. The flavinmoiety of FMN binds in a pocket formed by loops at the C-terminal ends of b-strands 3 and 4 (loops 4 2–4 9 and 7 1–7 9, respectively), known in Fld as the W and Y loops, or the 50s and ... than in flavodoxins. We have determined the crystal structures of NrdI from Bacillus anthracis, the causative agent of anthrax, in the oxidized and semiquinone forms, at resolutions of 0.96 and...
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Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc
... [10,11], and the VanR–VanS systemactivates the expression of vancomycin resistance[12,13]. Phosphate control of the production of actino-rhodin and undecylprodigiosin in S. lividans and S. coelicolor ... between the purified cytoplasmic domain of SenS (SenSc) and SenR. Using designed mutant pro-teins, the phosphorylation sites within SenSc and SenRhave been investigated. Bandshift and footprintinganalyses ... rapid and sensitive method for the quantification of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 24 8–2 54.43 Laemmli UK (1970) Cleavage of structural...
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Báo cáo khoa học: Electron-transfer subunits of the NiFe hydrogenases in Thiocapsa roseopersicina BBS pptx
... membrane and can be easilyTable 1. Activities of Hyn hydrogenase in vivo and in vitro in the presence and absence of the Isp1 and Isp2 proteins. The resultsare given as percentages of the level ... electroncarrier) and in vitro (in the presence of redox viologendyes). The data in Table 1 unequivocally prove that the in vivo H2-producing activity of the isp1,2 mutantstrain is completely lost and the ... reduced the in vivo activities of the hydrogenases,although they were fully active in vitro. The absence of HupC resulted in adecrease in HupSL activity in the membrane, but removal of the Isp1 and Isp2...
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Báo cáo khoa học: A biophysical view of the interplay between mechanical forces and signaling pathways during transendothelial cell migration doc
... talin, which binds the integrin to an actin fila-ment [16]. Stretching talin activates vinculin, a FA pro-tein, leading to reinforcement of the FA [17] (Fig. 1).Therefore, a rearrangement of the ... direction of shear stress. In (C), the force is in the direction of tension of actin filaments, maintained with the help of neighboring cells in contact. In (D), the force is in the direction of pulling ... completedvia integrin binding at FAs, leading to stretching of talin and activation of vinculin to reinforce the FA (D). The ECs respond to this interactionby forming stress fibers that contract, allowing...
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Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
... polymorphisms in the UGT1A7 and UGT1A9 glucuronidating enzymes in Caucasian and African-American subjects and theirimpact on the metabolism of 7-ethyl-10-hydroxycamp-tothecin and flavopiridol anticancer ... 33 is involved in the interactions of the enzyme with the substrate in bothUGT2B4 and UGT2B7. In contrast to the F33L mutation, which reduces the activity of UGT2B4 and UGT2B7, exchanging F33 ... Vmaxvalues. On the other hand, the Vmax of the mutanttowards 17-epiestriol was slightly increased and the Kmwas not significantly modified.Replacement of the Y33 residue of UGT2B7 by phe-nylalanine...
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Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf
... Kallio-Ratilainen and Johanna Aura for their technical assistance and Dr Xiaoyan Wu forassistance in protein purification. The Academy of Finland and the National Technology Agency of Finland, TEKES, ... between the C-terminal Glu (or Asp) of b-strand B6 (BAX and BCX have Asp) and Arg of the loop between b-strands B7 and A6. Thermophiles tend tohave more salt bridges, but on the other hand mesophilicTRX ... Joensuu, Finland;2Helsinki University of Technology, Finland The crystal structures of thermophilic xylanases fromChaetomium thermophilum and Nonomuraea flexuosa weredetermined at 1.75 and 2.1...
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt
... &CC( 7–8 ) steps (Fig. 2B) of the DNA strand, and GA(2 3–2 4) (Fig. 2C) and AT(2 4–2 5) (Fig. 2D) steps of the PNA strand, flanking the A24 C7 mismatch in PDwt(Scheme 1), and the corresponding AT( 6–7 ) ... destabilization and hence, diminution of the melting temperature (Tm) in the former.Base stacking in the vicinity of A C mismatch in PNAÆDNA and DNA duplexesIntra strand base stacking at the AC( 6–7 ) ... (a) reduced binding enthalpy caused by the loss of hydrogen bond and weak stacking and also (b) entropic and enthalpiccontribution resulting from the nature of interaction of water in the vicinity...
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Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein pot
... Asn2 and Asn15,predominantly express the structure Mana 1–6 (GlcNAcb 1–2 Mana 1–3 )Manb 1–4 GlcNAcb 1–4 GlcNAc. In the case of bovine, human and frog rhodopsin, both of these sites areglycosylated. On the ... conformational change in the polypeptide chain. Examination of the N-glycan structures provides insight into the processing of sugarchains in molluscs (Fig. 5) In this context, the question of whether the ... that the GU(ODS) and GU(amide) of a given PA-glycan can be represented by the sum of the contribution of each component monosac-charide unit. The UC values of the a 1–6 -linked and a 1–3 -linked mannose...
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Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc
... obtained with the transition at posi-tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the othertwo ... found in the 23S rRNA variant containing the double mutationU1911C ⁄ U1915C (Fig. 3). Thus, isomerization of uri-dines 1911 and 1917 in H69 occurs autonomously of each other and is independent of ... in the presence of 10 mm arabinose and made competent by con-centrating ten-fold and washing five times with ice-cold10% glycerol. Selection for the recombination event and the elimination of...
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Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx
... report the binding potentials between the phenol-hydro-xyl group of bisphenol A and estrogen-related receptor c residues Glu275 and Arg316 in the ligand-binding domain. By inducing mutations in otheramino ... structure BPA and its ball -and- stick structure,together with a space-filling structure in the ligand-binding pocket of the ERRc. The space-filling structure of BPA originated from the X-ray crystal ... 3D). The results clearly indicate that Glu275 and Arg316are crucial for the binding of BPA, and thus their sidechain carboxyl and guanidino groups are indeedengaged in hydrogen bonding with the...
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