Báo cáo khoa học: Experimental and steady-state analysis of the GAL regulatory system in Kluyveromyces lactis docx

... designs of the GAL system. It is of interest toascertain the role of autoregulation in the synthesis of activator protein KlGal4p in K. lactis as the synthesis of the corresponding activator in S. ... nucleus and cytoplasm; (d) the nuclear conﬁnement of KlGal80p; and (e) the fact thatKlGAL4 is the only gene in the GAL system with onebinding site, with the remaining genes having twobinding sites.Although ... activator KlGal4p plays a vital role in the induc-tion mechanism of the GAL system. The KlGAL4 genecontains a UASG in its own promoter for the binding of KlGal4p, resulting in an autoregulatory...

Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... oppositeGlu144 on the other side of the scissile glycosidic bond,contributes to catalysis by promoting distortion of the )1sugar and by increasing the pKa of the catalytic acid. The hydroxyl group of Tyr214 ... is no example of mutational analysis of all of these residues in the sameenzyme.To obtain an insight into how the many conservedresidues in the active sites of family 18 chitinasescontribute ... afﬁnity of ChiB for allosamidin, which is ananalogue of the proposed oxazolinium ion intermediate [32]. The minor (or even positive) effects of Y214F on substrate and intermediate binding and the...

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... hydrolysis of the nucleotide. To investi-gate the in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins, Pex1p and Pex6p carrying mutated ATP-binding sites ... do interact in vivo. We assigned their corresponding binding sites and elucidated the importance of ATP-binding and -hydrolysis of Pex1p and Pex6p for theirinteraction. We show that the interaction ... weretested for interaction with the binding partner in the yeast two-hybrid system. In the ﬁrst set of mutants, the lysine residue in the GXXGXGKT sequence of either one of the two WalkerA motifs...

Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

... buried in the interior of the protein molecule,to the ionone ring of retinol would be expected. Inter-estingly, binding of retinol caused an increase of the emission of the single Trp residue, ... In addition to the proteinpeaks, they also contained IAEDANS or IANBD bands,indicative of covalent binding of the dyes [19].Binding activities of Ag-NPA-1Binding of fatty acids, retinol and ... result in quenching of protein Trp ﬂuorescence and in anincrease of the speciﬁc DAUDA emission after the noncovalent incorporation of the ligand. Binding of DAUDA to Ag-NPA-1 caused  20% quenching...

Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx

... within the flower. The presence of cardosins A and C in the initial stages of flower devel-opment is in agreement with the presence of cyprosin B in flower meristems, as the antibody used in the immunocytochemical ... cardosins A, C and D,which extends beyond the coding region (Fig. 3), and the relevance of the region of 529 bp in cardosin Aexpression may explain the ineffectiveness of the cardosin D flanking ... DiscussionCloning by library screening of four full-length genesencoding cardosins A, B, C and D precursors, togetherwith the cloning of a partial sequence of the cyprosin Bgene and the isolation of the...

Báo cáo khoa học: Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395 docx

... with the E12value being the midpoint of these E1 and E2values [35]; and that for the FAD domain of P450 BM3;eE12value cited for the FAD domain of P450 BM3 is the midpoint of the E1 and ... differences in the environment of the FAD cofactor in the different proteins.These differences might also be manifest in the semiquinoneforms, as the relative intensities of the semiquinone signal in F1395A/S ... 1. The coenzyme-binding site in nNOSFAD domain and sequence alignments around the conserved aromatic residue in this domain.(A) The nicotinamide-binding site of nNOSFAD domain showing the...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved in coordinating a putative ... solutions. The ligandbound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car-bonyl. Binding of a xanthine base from XMP in the same position, would lead to the ... and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 ﬁlter...

Tài liệu Báo cáo khoa học: "Subjectivity and Sentiment Analysis of Modern Standard Arabic" doc

... best-performing results across the N-GRAM fea-tures and their combinations. In each case, our base-line is the majority class in the training set.3.1 SubjectivityAmong all the lemmatization settings, ... settings, a development setting (DEV) and a testsetting (TEST). In the development setting, we run the typical 5 fold cross validation where we train on4 folds and test on the 5th and then ... are reduced to their lemma citation forms, forinstance in case of verbs it is the 3rd person mas-culine singular perfective form; and (3) Stem, whichis the surface form minus in ectional morphemes,...

Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot

... 1c-ActinActin, cytoplasmic 1Tubulin b Tubulin aAnkyrin repeatdomain-containing protein 18AAnkyrin repeatdomain-containingprotein 18AHeat-shock protein b1a-Actin-2UnclassiﬁedKeratin, ... 14-3-3,either directly or indirectly. Analysis in detail of identi-ﬁed 14-3-3-binding proteins showed that more thanhalf of the proteins lost their ability to bind in C2-ceramide-induced HeLa ... staining of pro-teins in cell extracts suggests that some proteins couldbe cleaved during apoptosis initiation; this fact sug-gests an interesting hypothesis concerning the role of 14-3-3-binding...

Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate speciﬁcity doc

... that the thiol group of Cys135 adds to C6 of the pyrimidine ring, favoring the exit of the aminic group. The subsequent addition of a water molecule and the expulsion of the activecysteine complete ... environment in which it thrives in, leadsto the hypothesis of the existence of a reduced thiaminbiosynthetic pathway. Indeed, degradation products of thiamin [18] can be present in the stomach duringdigestion ... involved in thia-min biosynthesis, plays another relevant (despite stillnot being characterized) role in H. pylori and otherbacteria.Finally, the pivotal role of TenA in the thiamin bio-synthetic...

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