... of Illinois at Urbana-Champaign, Urbana, IL USA2 Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore3 Division of Environmental Science and Engineering, ... 2-isopropylalinine (2IPA), aniline and 2,4-dimethylalanine (24DMA) by E. coli JM109 expressing the wild-typeAtdA enzyme and the V205A and I248L mutants.AtdA32IPA Aniline 24DMARate(nmolÆmin)1Æmg)1protein)RelativerateRate(nmolÆmin)1Æmg)1protein)RelativerateRate(nmolÆmin)1Æmg)1protein)RelativerateWT ... glutamate and its releasefrom glutamine, respectively, AtdA1 and AtdA2 maybe involved in the recognition and release of anilineamino groups [12]. Hence, a similar engineeringapproach with AtdA1 and...