Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt
Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt
... December2009)doi:10.1111/j.1742-4658.2009.07555.x The present study aimed to investigate the role played by the leaflets of the plasma membrane in the uptake of drugs into cells and in their extrusionby P-glycoprotein and multidrug resistance- associated ... accu-mulation of dye in the cytoplasmic leaflet of the plasma membrane and release into the cell interior. The initial binding of dye to the cells, evident as the TMRM-mediated initial drop in TMA-DPH ... quenching could be the result of TMRM transfer from the cell surface furtherinto the outer leaflet of the membrane or flip-flopacross the membrane and residence in the inner leaflet of the membrane. ...
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Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt
... glycoprotein [28,29], in uenzavirus hemagglutinin [30,31], and IgM [32] in ER, a further role of N-linked oligosacharide chains of fhx/P25 in the process of assembly of the elementary unit in ER ... assembly of the elementary unit of fibroin in ERIt was demonstrated in this study that the elementaryunit of fibroin, having the same molar ratio of H- and L-chains, and fhx/P25 as of the elementary ... fhx/P25 in the elementary unit in the presence of L-chainTwo possibilities exist with respect to the role of L-chain in the protection of terminal a1,2-mannose residues of fhx/P25 in the elementary...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... tubulin partitioning into the detergent phase(HAT) is the acetylated tubulin forming a complexwith H+-ATPase. The finding of the H+-ATPase–acetylated tubulincomplex in plasma membranes of ... comparison of blots, it can beseen that most of the acetylated tubulin and ATPasewere involved in the immunoprecipitation process. Bymeasuring the intensity of the ATPase band in Fig. 5B(lane ... and the findings describedbelow, suggest that the decrease of HAT in the plasma membrane should be interpreted as a lower level of the H+-ATPase–tubulin complex. We therefore presumethat the...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... an 8 0-fold increase in laccase produc-tion. The identity of the recombinant protein was furtherconfirmed by immunodetection using Western b lot analysis and N -terminal sequencing. The m olecular ... strain ss3 of P. cinnabarinus. In order to improve the secretion of the recombinantlaccase, the laccase cDNA was fused to the GLA prepro-sequence and the production level markedly increased, ... activity (%)pHFig. 8. Effect of the pH on the activity of the purified laccase. pH in the range of 2.5–8 were tested with 500 lMABTS (d )and1 7lM of syringaldazine ( j) as the su bstrate.Sd 194...
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Báo cáo khoa học: Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus pptx
... towards the flavin cofactor is possibleonly in the dimeric structure of NADH oxidase. None of the tryptophan residues is close to the binding site of the flavincofactor in the monomeric form of the ... nitrogen of Trp47 and to O2 of the ribityl chain and is located in the middle of the flavin system with distances of 3.3 A˚toN1 0and3 .7A˚to N5. Arrows indicate the possible flipping move-ment of the ... time-dependence of NADH oxidation monitoredat 340 nm in the absence and in the presence of 1.25M and 4Murea. Surprisingly, the activity of NADH oxidase isincreased in the presence of urea and reached...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... control the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering ... distribution,making them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and b-catenin without ... (11). In this manner, the bacteria could access integrins and inject CagA (12). Injected CagA can then induce the massive disruption of cell junctions(13) and a loss of cell polarity (14). The induction...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the ... binding motifs common to most tyrosinasesequences [3] that contain five of the six copper-bind-ing histidine ligands. The sixth histidine ligand found in tyrosinases typically occurs before the ... whether the C-terminal domain in the plant and fungal enzymes has a purely inhibitoryfunction and ⁄ or a role in membrane binding is unclearat present. With regard to V. spinosum pro-tyrosinase,sequence...
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Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt
... whichcatalyzes the production of ROS and results in efficientkilling of invading pathogens [5]. The effect of the calmodulininhibitor W7 on fMLP induced ROS production wasinvestigated and compared with the ... counteracts the calcineurininduced affinity switch of a5b1integrin in CHO cells,suggesting also a role for Ca2+/calmodulin-dependentmolecules in regulating the affinity state of integrins onneutrophils ... calmodulin and PtdIns3K. In guinea pig neutrophils, it has been demonstrated thatinhibition of calmodulin results in inhibition of Rac and p21activated kinases [50]. Involvement of Rac in ROS...
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Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
... of the active site was better in the protein bear-ing the N2 domain than in the protein bearing the Ncdomain. Thus, the accessibility of the active site in the N2 proteins was independent of ... of the water–lipid interface. Also, both the N-terminal and C-terminal domains of hoPL wereinvolved in the inactivation of the protein at the water–triglyceride interface. The inactivation of ... investigate the role of the two domains of PLRP2 in the function of the protein, two chimeric proteins were designed by swapping the N and C structural domains between the horse PL (Nc and Ccdomains) and...
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Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
... Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNaseCarmine Ercole1, Francesca Avitabile1, Pompea Del Vecchio1, Orlando ... result alsoprovides indirect evidence of correct pairing of the inter-chain disulfide bridges in the recombinant sample [11]. The kinetics of the N-terminal arms swapping in the P19A-BS-RNase ... into an apparent molar heat capacity takinginto account the instrument calibration curve and the buffer–buffer scanning curve, and by dividing each datapoint by the scan rate and the protein...
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Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdflam the nao de tom tat bao cáo khoa hocbáo cáo khoa họcbáo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXBT Tieng anh 6 UNIT 2Trách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ