Báo cáo khoa học: The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation pdf

... 905–917 ª 2011 The Authors Journal compilation ª 2011 FEBS 917 The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation Yun-Jung ... MO1 and MO2 indeed blocked the expression of PRMT1 protein in zebrafish embryos, both effectively and persistently.Because PRMT1 is the predominant type I protein arginine methyltransferase, the ... 48 and 72 hpf as the source of protein arginine methyltransferase and recombinant mouse fibrillarin as the methyl-accepting protein. The samples were separated by SDS ⁄ PAGE and the methylated proteins...

Báo cáo khoa học: The Rieske protein from Paracoccus denitriﬁcans is inserted into the cytoplasmic membrane by the twin-arginine translocase doc

... questionwhether the Tat translocon is especially ‘permissive’towards the Rieske protein, allowing for variation at the ﬁrst arginine position, or whether the stricter roleof the second arginine is ... calculated for the ISP sequences and the comparison group(Fig. 2). Both curves display a positive score in the h-region which corresponds to relative hydrophobicity. The data show that the ISP group is ... elevated ISP amounts. Exchangeof the ﬁrst invariant arginine (R15K) leads to a com-parable distribution, but the total ISP level is clearlydiminished. In contrast, both the R16K mutation and the...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... HIS-PTI1-4 for the indicatedtime points. The mixes were then incubated with [c-32P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assayvisualized by autoradiography and the bottom ... One tenth of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeated twice usingrecombinant proteins prepared ... OXI1 and PTI1-4kinases were histidine (HIS)- or glutathione S-transfer-ase (GST)-tagged and puriﬁed from Escherichia coli.After mixing together HIS-OXI1 and GST-PTI1-4proteins or HIS-PTI1-4 and...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: intersubunit interactions during virus assembly doc

... SpainIntroduction During HIV-1 morphogenesis [1,2], the capsid protein (CA; or p24) participates in two distinct assemblyevents. The ﬁrst occurs inside the cell and involves the Gag polyprotein, of ... membrane, and the remainingdomains forming the innermost protein layers accor-ding to their positions in Gag: MA, CA-NTD,CA-CTD, the spacer peptide SP1, NC, the spacerpeptide SP2 and the C-terminal ... helices 9 and 10 of the other monomer. NoMHR residues are involved. The structural descriptionof the dimerization interface in the isolated CTD dimer is fully consistent with descriptions of the...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: designing inhibitors of capsid assembly ppt

... highest afﬁnity for the whole CA has the sequence IPVGEIYKRW, whichcorresponds to the a-helix 7 of NTD. This region is important for the formation of CA hexamers [6]; there-fore, this peptide could ... architecture is the production of Gag and Gag-Pol fusion proteins. The GagPol fusion protein, formed by a ribosomalframe shift event, is cleaved into the Gag and Polpolypeptides by the viral ... capsidinvolves targeting the whole CA protein and regionsclose to the CTD. Thus, these peptides and smallorganic molecules, together with the structures of the complexes that they form with CA, provide...

Tài liệu Báo cáo khoa học: The lipid ⁄ protein interface as xenobiotic target site Kinetic analysis of tadpole narcosis ppt

... 2 for the other tested alcohols.Statistical qualityAlthough the focus of the mathematical approach is on the ‘leftward shift’, illustrated in Figs 1 and 2, a statisti-cal analysis was performed ... multiple-sitekinetic formalism for lipid-dependent proteins hastherefore previously been applied to the nAChR [23] and is now applied to tadpole narcosis. General anaes-thetics are well known ... lipid activator sites of the nAChR. This is illustrated for 1-hexanol in Fig. 1. An approximationto the data points for tadpole narcosis and the ‘left-ward shift’ is achieved by increasing...

Tài liệu Báo cáo khoa học: The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC doc

... of KdpDin the absence and in the presence of CCCP. These resultsdemonstrate that the pmf is required for efﬁcient membraneinsertion of KdpD with the tags. This is in agreement with the wild-type ... stimulate the transloca-tion ATPase of SecA [6,38]. The result obtained here thatKdpD is independent of SecA is therefore consistent withprevious ﬁndings. The results obtained for the KdpD protein ... mM) for 5 min or by the addition of 2.5 lLofCCCP(10 mM) for 45 s, prior to labelling of the cells.ResultsMembrane insertion of the KdpD protein The membrane insertion of the KdpD protein is...

Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: interactions of HIV-1 capsid with host protein factors ppt

... proteomics of the capsid protein, its inﬂuence on the packaging of nonviral molecules into HIV-1virions and the subsequent role of the molecules themselves. These inter-actions and their characterization ... cap the top and bottom of the barrel (Fig. 3) [36,37]. The active site, in a hydrophobic pocket on the protein sur-face, is the binding site of CsA and its analogs. Muta-tional analyses and ... structural changes in the CA-CTD, as is evident by the inability to form aC198-C218 disulﬁde bond, which is surprising consid-ering that87HAGPIA92 is located in the CA-NTD[46]. The latter result...

Báo cáo khoa học: The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of b-defensins in A549 cells potx

... treatedwith 0, 20, 40, 60, 80 or 100 lgÆmL)1LPS for 24 h and then the RNA and protein were isolated for RT-PCR and western blot analysis. The results demon-strated that LPS induced HBD-2 ... target the HBD-2 pro-moter. Co-IP and ChIP experiments demonstrate thatHMGN2 does not interact with NF-jB directly and the two proteins do not form a complex in the nucleus and or share the same ... [28,44]. Based on thesedata, we propose that HMGN2 and p65 mutually rein-force their speciﬁc binding to the chromatin in the pro-moter of the HMGN2 gene, thereby regulatingHMGN2 protein levels...

Báo cáo khoa học: The astacin protein family in Caenorhabditis elegans docx

... Germany) to diminish the mutation rate inherent to the PCR reaction. The cyclingconditions were 94 °Cfor3min,94°Cfor40s,55°Cfor40 s, 68 °C for 1 min per kb for 35 cycles, and 68 °Cfor8 min. After ... evolutionary history of the astacin protein family in the nematodes. The identical and complex arrangement of the seven regulatory domains in NAS-39 and BMP-1 suggestsfurthermore that this distinct structure ... elegans surpassesany other organism studied so far. This investigationtherefore was stimulated by the question, what for this959-cell organism would need more than 30 different and active astacin...

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