Báo cáo khoa học: Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes docx

... FEBS Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes Jun Inoue, Yuka Ito, Satoko Shimada, ... the addition of glutamine, the gene expression of the SREBP targets,such as HMG CoA synthase and the LDL receptor,was slightly increased, but then the mRNA levels of both SREBPs and their target ... that the larger the amount of glutamine added to the med-ium, the more the expression of genes related to lipid homeostasis is pro-moted by the activation of sterol regulatory element binding...

Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot

... Elevated serine ⁄ threonine phosphorylation of IRS-1 and IRS-2 inhibits their binding to the juxtamem-brane region of the insulin receptor and impairs their ability to undergo insulin-induced tyrosine ... been the subject of intensive research for many years. Yet, wedon’t know the details of the molecular dysregulationin the target cells of the hormone. Studies of cells frompatients with the ... 1C).Directly after their isolation, adipocytes respondedto insulin by increasing the uptake of 2-deoxyglucose.Neither the maximal effect of insulin on glucoseuptake and hence the amount of GLUT4 (M....

Tài liệu Báo cáo khoa học: How disorder inﬂuences order and vice versa – mutual effects in fusion proteins containing an intrinsically disordered and a globular protein docx

... (inthis case on the tumbling of the rigid part), with the extent of the perturbation being proportional to the length of the disordered region.With the aim of investigating the reciprocal confor-mational ... of disordered and ordered moieties within the fusion proteins, wecalculated the theoretical average spectra of equimolarIDP and GFP mixtures by averaging the spectra of the individual IDP and ... Far-UV CD spectra. The CD spectrum of each of the fusionproteins is compared with that of individual proteins, with the theo-retical average spectrum and with the spectrum of equimolar pro-tein...

Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

... conditions [22], butwith the wall of the pore made of both lipid and pro-tein molecules. At the rim of the membrane hole, the two leaﬂets of the bilayer can make contact with eachother, forming a membrane ... This softwareallows the use of different reference sets of proteins, includingmembrane proteins, increasing the reliability of the analysis.AcknowledgementsThis work was supported by the Spanish ... activity of a pep-tide derived from the second helix of the putativepore-forming domain of Bax. We show that peptideswith the sequence of the ﬁrst (a5) and second (a6) heli-ces of the Bax...

Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx

... analysed the thermal unfolding proﬁle of lysozyme in the presence of additives. Figure 3A shows the thermal unfolding curve of lysozyme in the presence of ArgEE as m onitored by DSC. In the absence of ... to Argis related to the ethylation of the main chain of the carboxylgroup. Figure 5B shows the amount of aggregation of lysozyme at pH 6.5 and 10.0 in the presence of Arg and ArgEE after heat ... equation and determined the apparent Tm.ResultsThermal inactivation and aggregation of lysozymein the presence of additivesFigure 1 s hows the thermal inactivation and aggregation of lysozyme...

Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

... regarding the identiﬁcation and distribution of TLP genes in nature, the structural and functionalcharacterization of the TLP from various organisms, and the evolution of TLP and transthyretin. The ... in the peroxisomes of metazoan and plant species, and the co-regulation of TLP genes in some bacteria, suggests a concertedeffort in the rapid generation of allantoin. The co-dis-tribution of ... some stage during their development[2–4] and this synthesis is primarily localized to the liver, choroid plexus and retinal pigment epithelium. The expression of the transthyretin gene in vertebratesoccurs...

Báo cáo khoa học: Post-ischemic brain damage: pathophysiology and role of inﬂammatory mediators ppt

... levels of cytokines and chemokinesenhance the expression of adhesion molecules on cerebral endothelial cells,facilitating the adhesion and transendothelial migration of circulatingneutrophils and ... [24–26]. Thesemediators induce the expression of the adhesion mole-cules ICAM-1 [27–30], P-selectin and E-selectin [31,32] and integrins [33,34] on endothelial cells and leukocytes,which promote the ... after the insult and remain elevated fordays [45–49]. The expression of these pro-inﬂammatory genes is regulated by transcription factors that arestrongly stimulated by the ischemic insult and...

Báo cáo khoa học: pH dependence, substrate speciﬁcity and inhibition of human kynurenine aminotransferase I ppt

... picture of the enzyme and should be helpful in a comprehensive under-standing of the role of hKAT-I, especially in KYNAbiosynthesis in the human brain.Experimental proceduresEnzyme expression and ... by their rather low afﬁnity to the enzyme.Effects of other keto acids on hKAT-I activityAbility to function as the amino group acceptor for anumber of biologically relevant keto acids and their substrate ... shown).Incorporation of a-ketobutyrate or other a-keto acidconcomitantly decreased and increased the 335 nm and 422 nm peak, respectively, and the relative dimensions of the pyridoxamine peak (335 nm) and enzyme-PLP...

Báo cáo khoa học: Systems biology: model based evaluation and comparison of potential explanations for given biological data pot

... mentioned above, the sharedfeatures are the total number and the values of the data points themselves, and the variation is given by the number of times the data points appear.Another type of bootstrap ... and the iteration of the above mentioned analyses with the experimental datagathering phase is the topic of an accompanying mini-review [56], and is outside the scope of the presentone.SoftwareAn ... majority of the residuals are similarto their neighbours (e.g. if the simulations lie on the same side of the experimental data for large parts of the data set), the model does not explain the data...

Báo cáo khoa học: Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase docx

... isomerase and many other proteins. The active site of enolase is presentat the C terminus of this barrel. The small or N-terminaldomain wraps around the outside of the main domain [8].Most of the ... possibilities for the design of selective inhibitors, although their presence in the secondshell of the catalytic site, rather than in the ﬁrst (Fig. 4)raises doubts as to their accessibility ... to the 5¢ end of the enolase gene; and 5¢-CGCGGATCCATATCCGTTACGACCACCGGG-3¢, complementary to the 3¢-terminal codingregion of the gene, followed by a BamHI restriction site(underlined). The...

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