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Báo cáo Y học: Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge pptx

Báo cáo Y học: Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge pptx

Báo cáo Y học: Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge pptx

... performed by Edman degra-dation (PE Applied Biosystems, model 47 3A) on native andon reduced and pyridylethylated peptides. Bacterial challenge of white bass and RNA samplingThe challenge experiment ... 90) is probably the translational start sitebecause it is followed by a typical signal peptide motif with a basic residue (lysine) and a hydrophobic region (rich invaline and alanine) and matches ... Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge Hiroko Shike1, Xavier Lauth1, Mark E. Westerman2, Vaughn E. Ostland2, James M. Carlberg2,Jon C. Van...
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Báo cáo Y học: Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin a-sarcin potx

Báo cáo Y học: Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin a-sarcin potx

... acid and base on the catalysis by a- sarcin [19,20],rendered proteins with no detectable activity against ApA[20]. Cleavage of ApA by a- sarcin is a low-specificityreaction requiring high amounts ... catalysis rather than in substrate binding. Therole of Arg77 has not yet been established as all attempts toisolate any RNase T1 Arg77 mutant have been unsuccessful[23]. Hydroxylamine treatment ... ribonucleaseactivity of a- sarcin has been developed by using thedinucleotide ApA as substrate [18]. Both mutant variantshydrolysed ApA. They displayed a Kmvalue similar tothat of the wild-type protein although...
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Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

... IETD-CHO,N-acetyl-Ile-Glu-Thr-Asp-aldehyde; IETD-AMC, N-acetyl-Ile-Glu-Thr-Asp-AMC; LEHD-AMC, N-acetyl-Leu-Glu-His-Asp-AMC;YVAD-AMC, N-acetyl-Tyr-Val-Ala-Asp-AMC; z-VAD-fmk, ben-zoyloxycarbonyl-Val-Ala-Asp(OMe) fluoromethylketone.(Received ... purchased fromBiomol. Ac-DEVD-AMC is a substrate for caspases 3and 7; Ac-YVAD-AMC is a substrate for caspase 1;Ac-IETD-AMC is a substrate for caspase 8 and 10;Ac-LEHD-AMC is a substrate for caspases ... KIPase activity is a novel caspase-like activity associated with cellproliferationCahora Medina-Palazon, Emmanuelle Bernard, Victoria Frost, Simon Morley and Alison J. SinclairBiochemistry...
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Báo cáo khoa học: Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex pptx

Báo cáo khoa học: Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex pptx

... homologs are poly (A) polymerases. Proc Natl Acad Sci USA 101, 4407–4412.18 Nakanishi T, Kubota H, Ishibashi N, Kumagai S,Watanabe H, Yamashita M, Kashiwabara S, Miyado K& Baba T (2006) ... separated by SDS-PAGE and analysed by western blotting.Polyadenylation assayOocyte total RNA was extracted using the Mini RNA Isola-tion IITMKit (Zymo Research, Cambridge, UK), and PATwas carried ... poly (A) tail length by cytoplas-mic polyadenylation is critical for the regulation of specific mRNA expres-sion. Gld2, an atypical poly (A) polymerase, is involved in cytoplasmicpolyadenylation...
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Báo cáo Y học: Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase docx

Báo cáo Y học: Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase docx

... [13].Activity assaysEnzyme catalyzed reactions were monitored continuouslyon a Hewlett-Packard 8453 diode-array spectrophotometer.Ferric reductase activity was assayed in both aerobic andanaerobic ... iron availability in thehost plays a very important role in promoting the infection by mycobacteria. Interestingly, it has been reported thatNramp1 (natural resistance-associated macrophage ... species (FAD-NADPH and FADH2-NADP+) and FAD semiquinone.Unlike bovine AdR, FprA highly favored the CT speciesFAD-NADPH, as judged by comparison of the spectra [21]. By analysis of the...
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Báo cáo Y học: Concentration-dependent reversible activation-inhibition of human butyrylcholinesterase by tetraethylammonium ion pptx

Báo cáo Y học: Concentration-dependent reversible activation-inhibition of human butyrylcholinesterase by tetraethylammonium ion pptx

... carried out thesteady-state and progress curve analysis of BTC hydrolysis by recombinant wild-type human BuChE, by four selectedmutants (Y3 3 2A/ D70G, Y3 32D/D7 0Y, W8 2A, A3 2 8Y) and by commercial ... to statistically anticipate the real value unless theclear plateau is reached [23].The initial rate data for the W8 2A mutant differedsubstantially from the data for other enzymes. It appearedthat ... Swiss-Model, an automated protein modelingFig. 1. pS c urves for the hydrolysis of butyryl-thiocholine c atalyzed by the w ild-type, by v ar-ious human butyrylcholinesterase mutants andby...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx

Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx

... 5¢ TAG TTC AAA TGC ACA TCT TAT 3¢C14U 5¢ GGC AGA GAA TAC GTT TAT ACT AA 3¢C14L 5¢ TCT CAG ATT GAC TAC TAC AAC TT 3¢C15U 5¢ CAT GAA AAG CAT TTC AAA AA 3¢C15L 5¢ TAA AAA ACA GTG GTT ACC AA 3¢C16U ... CAA GTT TAT AGT CTA CAC GTT GTA C 3¢C19U 5¢ CTT AGC ATC TTC CAT GGC TTG ATC 3¢C19L 5¢ ATA CAC ACA AAC TCA CAA GG 3¢MWU 5¢ CAA CAT ATT TTA AAT AAA ATT GAC AAT 3¢C9LM* 5¢ ATA AAA ATG GTA TAC ... CAT AAA ACT AAC AAT ACA TGT 3¢C1011L 5¢ TAG CAG ATA TTG AAA AGG AG 3¢C12U 5¢ CCA GTG AAT ATT CAG GAC TGA T 3¢C12L 5¢ AGG CTC TAG CAT GAT TTG ATG T 3¢C13U 5¢ GCA TTT TTA TTT TGA ATG TAA A...
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Báo cáo y học:

Báo cáo y học: "Do we need a critical care ultrasound certification program? Implications from an Australian medical-legal perspective"

... provide a AbstractMedical practitioners have a duty to maintain a certain standard of care in providing their services. With critical care ultrasound gaining popularity in the ICU, it is envisaged ... it is opined by most practitioners as acceptable or reasonable practice, it will not be accepted by the court (Box 1).Standard of care e requisite standard of care is reasonable skills and ... permit-ting a registrar to perform a vaginal hysterectomy that was beyond the capacity of the registrar in question. As a result of poor surgical skills, the patient’s fallopian tube was entrapped...
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Báo cáo y học:

Báo cáo y học: "Spinal Intramedullary Cysticercosis: A Case Report and Literature Review"

... anatomically as extraspinal (vertebral) or intraspinal (epidural, subdural, arachnoid, or intramedullary), of which the intramedullary type is quite rare and only fifty-three cases have been ... clinical manifestations included pain, paraparesis, spasticity, bowel and bladder in-continence, and sexual dysfunction1,20. However, in-flammatory reaction against the dead parasite is as-sociated ... reported a case of intramedullary cysticer-cosis at T4 and T5 vertebral level and discussed its diagnosis and treatment with literature review. Case Report A 40-year-old female patient was transferred...
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Báo cáo y học:

Báo cáo y học: "The Impact of a Nationwide Antibiotic Restriction Program on Antibiotic Usage and Resistance against Nosocomial Pathogens in Turkey"

... a Nationwide Antibiotic Restriction Program on Antibiotic Usage and Resistance against Nosocomial Pathogens in Turkey Adalet Altunsoy1, Cenk Aypak2, Alpay Azap1, Önder Ergönül3, İsmail ... G, Markogiannakis A, Papaparaskevas J, Daikos GL, Stefanakos G, Zissis NP, Avlamis A. Differences in the changes in resistance patterns to third- and fourth-generation cephalo-sporins and ... February 2003 by a central regulation of Ministry of Health and was announced nation-wide via official newspaper of the state [11]. This is a quasi-experimental study performed in four year...
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