Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx

... Proceedings of EACL '99 Determination of Syntactic Functions in Estonian Constraint Grammar Kaili Mfifirisep Institute of Computer Science University of Tartu Liivi 2, ... of syntactic analysis of Estonian using Constraint Grammar. Constraint Gram- mar framework divides parsing into two different modules: morphological disam- biguation and determination of ... less than 2% (Puolakainen, 1998). All the syntactic information is given by syntactic tags in constraint grammar framework. The syntactic tags of Estonian Constraint Grammar (ESTCG) are...

Báo cáo khoa học: Internalization of cystatin C in human cell lines docx

... cyste-ine protease, legumain, because of removal of thekey amino acid in the legumain-binding site of cysta-tin C [22]. The amino acid substitutions reside in opposing parts of the cystatin C ... containing 1 lmrecombinant cystatin C.Quantification of cystatin C uptakeThe determination of basal cystatin C levels was followedby measuring the cellular content of active cysteine proteaseinhibitor ... biolo-gical specificity of cystatin C. Identification of leucine 9 in the N-terminal binding region as a selectivity-confer-ring residue in the inhibition of mammalian cysteinepeptidases. J Biol...

Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

... requirement of three. The crystal structures of CTX–inhibitor complexes gave interesting insights into the binding. Surprisingly,none of the inhibitors occupied any of the aglycone subsites of the ... Quantitative determination of nonco-valent binding interactions using soft ionization massspectrometry. Int J Mass Spectrom 216, 1–27.54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact ... S-Xyl3-Me. In contrast, S-Xyl2-Medid not bind to any xylanase at the initial concentration of 5–200 lm, indi-cating increasing afﬁnity with increasing number of xylopyranosyl units,with a minimum...

Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

... Determination of the reopening temperature of a DNA hairpinstructure in vitroXuefeng PanInstitute of Microbiology, The Chinese Academy of Sciences, Beijing, ChinaA novel method, ... the sequen-cing primer. Determination of the hairpin reopening temperatureOligonucleotide primer 2 was mixed with single-strandedtemplate DNA at a ratio of 3 : 1 in the annealing buffer.This ... program[25]). (C) Illustration of the working mechan-ism for measuring the reopening temperature of a small DNA hairpin structure.Ó FEBS 2004 Reopening temperature of single DNA hairpin (Eur. J. Biochem....

Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

... otherthan collision of the two flavin-binding domains.DiscussionThe ability to dissect CPR into distinct functional domainshas assisted in providing detailed structural and kineticinformation about ... FMN domains of NR1 enabled the determination of the midpoint reduction potentials for theoxidized/semiquinone (E1) and semiquinone/hydroquinone(E2) flavin couples. Data for both domains were ... expression of theFMN domain.Recombinant protein expression and purificationFor expression of the various domains in Escherichia coli,strain BLR (DE3/pLysS) containing the appropriateplasmid strains...

Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

... Mixtures of lactose and (asialo)fetuin were expec-ted to be potent inhibitors. Carbohydrate-independentbinding of the neoglycoprotein and biotin-independentbinding of the second-step reagent was ... cell lines of diverse histogenetic origin. The median signal of binding of the ﬂuorescent probe is givenas extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent ... Theoccurrence of ricin resistance in CHO cell mutants beingattributed to GnT-III activity [15,16] is an example in which an ensuing decrease in afﬁnity in plant lectinbinding can help to explain the...

Báo cáo khoa học: Determination of the metal ion dependence and substrate speciﬁcity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

... p.p.m.Extinction coefﬁcients of HPDA and substituted HPDAwere determined by adding XylE and TodF stepwise tosolutions containing known amounts of catechol and deter-mining the resulting UV absorbance. ... Thebinding of divalent cations by the decarboxylase pre-cludes metal binding studies of the hydratase in thatcomplex. In this study, BphH, the hydratase in the PCBsdegradation pathway of B. ... catalysis.Number of metal ion bound per enzyme and determination of coordination numberThe equilibrium concentration of Mg2+bound toBphH was determined by atomic absorption spectro-scopy. A maximum of...

Báo cáo khoa học: " Connectability Calculations, Syntactic Functions, and Russian Syntax" pdf

... coordinative functions (coordination, apposition, etc.) remains to be written. 1. Introduction The subject of this paper is a certain kind of routine for testing the connectability of pairs of ... N(Neut), and N(Plu) are four nominal- properties segments stored with the CT routine and containing 1's in their masculine-singular, feminine- singular, neuter-singular, and plural positions, ... Y. In each cell of the table (see Fig. 2), the decrement is a string of zeros with a 1 in the position representing a certain function, and Y is the address of the first cell of a subroutine...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... shown that interactions betweenKCs and endotoxin comprise the initiating event lead-ing to hepatotoxicity in liver injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, ... mediator of lethal sepsis, triggering theproduction of proinﬂammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate therole of KCs in the regulation of CYP isoform ... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidativestress on the surrounding tissue, releasing largeamounts...

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... long-chain saturatedfatty acids (SFAs) led to increased expression of proin-ﬂammatory cytokines, inhibition of insulin signaling,induction of endoplasmic reticulum (ER) stress, andpromotion of ... elevations in theexpression of activating transcription factor 4 (ATF4)and of CCAAT⁄ enhancer-binding protein homologousprotein (CHOP) proteins downstream of eIF2a wereobserved (Fig. 4A), indicating ... Hoechst 33342(30 min, 37 °C). Annexin V–ﬂuorescein isothiocyanate(FITC) (BD Pharmigen, San Diego, CA, USA) binding andPI staining were performed according to [44]. Followingincubation with 1.0...

Xem thêm