... (Eur. J. Biochem. 270) Ó FEBS 2003
Analysis of the effect of potato carboxypeptidase inhibitor
pro-sequence on the folding of the mature protein
Sı
´
lvia Bronsoms, Josep Villanueva, Francesc ... min; then 10 lLofincreasing
concentrations of PCI or ProNtPCI were added and
the measures were continued for 2 min. The slope of the
first part of the ass...
... respectively, in the part of the cytoplasm
outside of the granules. FRAP analysis in the gran-
ules provided typical recovery curves of these fusion
proteins: a diffusion constant with two components
of 0.9 ... and to mea-
sure the mobility of the components in the PGCs. The
medaka embryo was peeled off the chorion, and the
segment containing the part of PG...
... localization. The N-terminus
has the biggest effect on localization; the C-terminus
plays a secondary role, determining the preferred local-
ization in the absence of the N-terminus and the Sm
domain ... under
stress conditions, showing that none of the Lsm1p
domains is required absolutely for this localization.
However, a strong reduction in P-body localization of
m...
... (Sigma-Aldrich).
Concentrations of ethanol in the reaction mixture did not
exceed 1%. Retinoid concentrations were determined on
the basis of the corresponding extinction coefficients at the
appropriate ... anchor the protein to
the ER membranes. Furthermore, we analyzed the
contribution of the C-terminal end to ER localization.
We fused the last 41 amino acids of...
... work. Rather, here we try to investigate the ef-
fect of the exact distribution of the language inven-
tory size on the nature of the degree distribution of
the consonants through a synthetic ... contributions, pointing out some
of the implications of the current work and indi-
cating the possible future directions.
2 PlaNet: The Phoneme-Language
Network
We d...
... that the introduction of
acidic residues at the positions of the phosphorylation
sites in 4E-BP1 decreases the interaction of 4E-BP1
with raptor. This implies that the regions of 4E-BP1
containing ... Some
degradation of the GST fusion proteins is
evident from the presence of products run-
ning at the position of GST itself. (E) Binding
of raptor to different a...
... the
maturation of the enzymes. Most o f them a ct pleiotropically
in the synthesis of all four hydrogenases, in particular in the
synthesis and insertion of the metal centre [6,7]. Two of the
products of the ... doi:10.1111/j.1432-1033.2004.04280.x
characterization relative to the substrate ATP. The analysis
of mutant variants of HypE demonstrates that the...
... to confirm the presence of the RIP/lectin coding
sequence in the tobacco genome. Only those plants that
were positive in the PCR analysis were withheld for further
analysis. Expression of the ... Inhibition percentages were calculated by the following
formula: 100 · (number of lesions wild-type – number of lesions
transformant)/number of lesions wild-type. When the n...
... trans-
ition temperature of the thermal denaturation. This
destabilization occurs with the dissociation of the het-
erodimer at low protein concentrations. Nevertheless,
the concentration of PSP-I⁄ ... presence of 3 mm CaCl
2
did not modify the
chromatographic behaviour of PSP-I⁄ PSP-II. In con-
trast, the addition of 2 mm Zn
2+
intensified the
deviation of the...
... equivalent
amounts of each of these proteins in the four samples.
Interestingly, Band 6, which corresponded to a band
excised in the vicinity of the 380-kDa region of the
first dimension BN-PAGE separation, only ... determine whether the
degradosome components seen in Fig. 2B were still present across the mass range. Band 6 was chosen on the basis of the apparent mi...