... aspects of comparative studies
at the subcellular level
In addition to the description of the proteome of a
subcellular entity, the analysis of dynamic proteome chan-
ges at a subcellular level promises ... post-translational modifications that affect the
migration behaviour of the protein on the 2D gel. Despite
the exceptional analytical power of...
... accordance with the
guidelines of the Ministry of Education, Science, Sports and
Culture of Japan for the use of laboratory animals.
Cell fractionation
Subcellular fractions of rat liver were prepared ... with partial digestion of the surface lamina of
the nuclear matrix to allow penetration of the gold
particles into the nuclear matrix [19] and the export
me...
... embryo-
genesis, all the evidence suggests that sex determination
might disobey the conventional rules of evolutionary
conservation. The common picture emerging here is that
the genes at the top of the cascade ... activity in the fish ovary [47,48]. Given the
abovementioned observation that estrogens repress male
differentiation it appears that, once initiated, factors o...
... degeneration of the caudate nucleus, putamen,
and occasionally the globus pallidus, with little involve-
ment of the rest of the brain.
The question of how mutations in the same gene or
group of ... euchromatin at the nucleoplasm, and of gene-silenced condensed
heterochromatin at the vicinity of the INM are circled. In the latter state, epigenetic modifica...
... resulted in an
increase in the ratio of intermediate to mature cyto-
chrome c
1
and a disappearance of the intermediate form
of the Rieske protein. At the same time, the levels of
subunits 7, 8 and ... around the
catalytic core of the enzyme to arrive at the three dimen-
sional organization revealed by the crystal structures
(Fig. 1A). The supernumerary sub...
... other amino acids
situated in the vicinity of these residues. The results
confirmed the importance of the amino acid residues, all
located at the putative catalytic domain, for the GCPII
hydrolytic ... °C
ATTCTCGAGTCATTATGCAACATAAATCTGTCTCTT
44/716 AAACTCGAGAGATCTAAATCCTCCAATGAAGC 30 s/94 °C; 1 min/56 °C; 4 min/72 °C
AAACTCGAGTTATTATTCAATATCAAACAGAG
59/750 AAAAGATCTAAAGCATTTT...
... ending at nucleotide 16 295 of the genome ([20]
see Fig. 1). Since the RNA is polyadenylated, it is uncer-
tain if the termination occurs at the C residue at 16 293
or the A residue at 16 295 of the ... populations of H-strand transcripts escape
termination at the mt-TERM and continue through the
distal sites of the genome.
Studies from two laboratories sho...
... studies demonstrate that miR-23a
potently promotes the growth of the gastric adeno-
carcinoma cell line MGC803, providing the first proof-
of- concept that there is a potential link between the
tumor ... expression. These data highlight the
prediction that IL6R is a direct target of miR-23a.
miR-23a negatively regulates IL6R expression at
the mRNA and protein levels
miRNAs c...
... the cleft of actin protomers located
in the vicinity of the barbed end of the filament, pro-
ducing an ATP or ADP–Pi cap at this end. Because of
the presence of this cap at the barbed end the critical
concentration ... while
at pH 6.5 the acceleration of the cleavage was lar-
ger in the absence of Pi than in its presence. We
also studied the effect of...
... postulate two pathways for the catabolism of
CH
3
-4-aminobutyrate that is generated from the side
chain of 2,6-dihydroxypseudooxynicotine [7]. The first
would start with the oxidative demethylation ... RT-PCR analysis of transcripts. (A) Schematic representation of the pAO1 gene and ORF
cluster flanked by Tn554 and DTn. The cluster consists of the pmfR gene, encoding...