Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
... Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 Emmanuelle ... analysis of native GAPDH shows that it is acomplex of GAPDH and the small protein CP12. In vitroreconstitution assays indicate that the kinetic differences are the result conformation changes of GAPDH ... mM. Recombinant GAPDH and CP12 reconstitutionexperimentsTo check whether the different kinetic parameters obtained for native and recombinant GAPDHs were linked to the presence of CP12 with native...
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Báo cáo khóa học: Comparison of native and recombinant chlorite dismutase from Ideonella dechloratans pot
... dechloratans, and the recombinant version of the protein produced in Escherichia coli, suggests the presence of a covalent modification in the native enzyme.Although t he native and r ecombinant N- and ... in Fig. 1)in the e lectropherogram of the n ative enzyme are missingin the electropherogram of the recombinant enzyme.There are also two peaks in the electropherogram of the recombinant enzyme, ... fit(Fig. 5 B). The major component, accounting for 92% of the total amplitude, t itrated with a pKavalue of 1 0.15 ± 0.0 3,in accordance with the pKavalue of 10.1 for tyrosine [35]. For the minor...
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Báo cáo khoa học: Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes pdf
... comparison of the residues serving as the ligand for the M3 metal ion is shown in Table 1. The residues of the direct ligands to the metal were the same as in hPLAP, but those of indirect ligands,which ... we used the swiss-model program toconstruct 3D models of rIAP-I and rIAP-II; we thenanalyzed the active site of the enzymes and studied the effect of various combinations of metals at the activesite. ... reported that the addition of Zn2+(0.01 mm) decreased the activity of TNAP isolated from the liver and the kidney, butincreased the activity of AP isolated from the small intestine. These findings...
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Báo cáo khoa học: Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans docx
... containingampicillin, and 0.5 mL of the culture was then inoculatedinto 50 mL of the same medium. A crude protein extractcontaining GST–mSPS1 and His–mDLP1 (or hDPS1 and hDLP1) was then prepared from the transformant ... nosBF180140 for the murine homolog and AI590245 and AI261617 for the human homolog) were selected. Wealso cloned the cDNAs with the highest homology toSpDlp1 (Accession nos BE283879 and AI097731 for the ... of pSTVK28. pSTVhDLP1 was digested with BamHI and HindIII and cloned into the same sites of pQE31 to yield pQhDLP1, which was in turn digested with XhoI and HindIII and cloned into the SalI and...
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Báo cáo khoa học: Characterization of depolarization and repolarization phases of mitochondrial membrane potential fluctuations induced by tetramethylrhodamine methyl ester photoactivation pdf
... abolished the rotenone-induced inhibition of R phases. Takentogether, these findings indicate that the activity of both respiratory chain and ATP synthase were required for the recovery of the mitochondrialpotential. ... that the activity of both respiratory chain and ATP syn-thase is required to activate the R phase. On the otherhand, the frequency of D phases was substantiallystable. The frequency of D phases ... as the interval between the zero-derivative timepoints t1 and t2. The peak symmetry was calculated as the lowest of the reciprocal ratios between the t2-p and p-t1 segments. Cut-off values for...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... moreweakly with the reagents currently available.To analyse further the composition of the PS1 complex,samples with high expression of PS1 complex were selected for further study. BN/PAGE gel bands ... to the generation of Ab peptides in pathological processes. Study of the characteristics of the PS complex will facilitateelaboration of the molecular processes involved in the production of ... PS1-deficient cells, and down-regulation of NCT inhibitsc-secretase cleavage of APP and Notch [11–13]. PEN-2 isa small double membrane-spanning protein with N- and C-termini facing the lumen [14]....
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... (i.e. the development of the gills and the production of an adult shell), which is accom-panied by significant growth of the soft body parts[21]. The mantle edge governs shell formation and body ... Metamorphosis represents the ulti-mate stage of oyster development, and is characterizedby the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues ... origin and a high con-servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPsin humans and insects [14], we hypothesized thathomologues of...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... and quantitativereal-time RT-PCR, the locations of the primers DN)105 and DN)9used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... fragments F and I. The number at the right of each RT-PCR product represents the length of the prod-uct. The scheme also shows the location of the reverse primerDN+89 used in another starting ... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbersin parentheses below the diagram show the position of the corresponding...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... butfurther enhances the activity of the enzyme whoseC-terminus has been displaced by low pH or byFC-induced binding of 14-3-3s.Results The C-terminus of isoform 1 of the PM H+-ATPase of A. ... XL10 and the frame and the identity of the cloned fragment verified bysequencing. The construct with the N-terminal portion of PPI1 has been previously described [22]. The DNA fragment coding for ... 0.1 lm for PPI1588His6 and 1.7 ± 0.2 lm for His6PPI188. Thus,all the following experiments were performed with PPI1588His6. The analysis of the effect of PPI1588His6on the dependence...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... primers. Both of the genes were constructed either with or without a His-tag at the 3¢ terminus of the coding region. Thus, the stop codon of SNO1 and SNZ1 was replaced with bases coding for the sequence ... in the presence of 1–10 mMglutamine and 30 lgof the complex at 30 °C for 10 min. The sample was thenboiledfor1minandkeptfrozenat)80 °C for subsequentanalysis. To quantitate the amount of ... N-terminusincluding the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p.Properties of the complex of Sno1p and Snz1pWhen Sno1p with a His-tag and Snz1p without a tag werecoexpressed and then...
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