... Lys33GP was constructed by the SOE-PCR strategy, using primer 1 (5¢-GTGAATTCCTGGAAGTTCTGTTCCAGGGGCCCAGCAGCGATGAACCGAGCGAAAGCAGCGAACCGAGCTGCGATAGCAGC-3¢) and primer 2 (5¢-GTCTCGAGTTACAGGCGAATATCGGCGCAATGGCACTGCGGCGGAATGCTTTTGGTGCAGCGGCTGCTATCGCAGCTCGG-3¢). ... ZORBAX 300 SB-C18 semiprepar-ative column was from Agilent Technologies (Santa Clara,CA, USA). Trifluoroacetic acid (TFA) and acetonitrile were from Merck (Darmstadt, Germany). All Fmoc amino acidswere ... B-III, of peanut(Arachis hypogaea). J Biochem 93, 479–485.6 Tanaka AS, Sampaio MU, Marangoni S, de Oliveira B,Novello JC, Oliva ML, Fink E & Sampaio CA (1997)Purification and primary structure...