... RanGTP [7,8]. In the cytoplasm, a secondRNase III, Dicer, cleaves the pre-miRNA to generate the mature miRNA [3,9,10]. The function of miRNAs and the ability to knockdown expression of specific genes ... followingmicroinjection of other types of RNA molecules con-taining the same GFP sequence. As expected from the minimal effects on embryonic development caused byinjection of equal numbers of these GFP-specific ... reduction in the level of processed miR-430b at 12 hpf (Fig. 2B).Consistent with the common MMB defects of the threesiRNAs, expression of the pax2a gene, which is anTable 1. Analysis of the efficiencies...