... comparison with 3-indolylacetonitrile, the rate of hydrolysis of 3-phenyl-propionitrile is increased by a factor of 330, and the Kmvalue is reduced by a factor of 23. With the exception of ¯uoro, ... (5¢-ACAATTGATGATTCAACGCCCAAC3¢). Using the BglII sites in the 5¢ overhang of AtNIT1-forand the 3¢-noncoding region of the cDNA, the AtNIT1cDNA was inserted in-frame in the BamHI site of pQE10. The resulting expression ... had the better stabilizing effect (Table 2). The loss of enzyme activity on the addition of 2 mMdithiothreitol was20% compared with 63% for the reference without thiol.However, increasing the...