Báo cáo khoa học: The N-linked oligosaccharides of aminopeptidase N from Manduca sexta pptx

... GlcNAc antennaeand the distal GlcNAc of th e chitobiose un it in N- glycans. Furthermore, the major N- glycan componentson APN1 carry antennae linked to the 6-arm of coremannose. This is in accord ... character-ization of the N- glycans on the major APN1 protein, asinspection of their amino acid sequences revealed sevenconsensus sequences for N- glycosylation in APN3 and ﬁvein APN4. Therefore, these ... APN2 isknown also to bind Cry1 toxins [13,14], no binding partnershave yet been identiﬁed for either APN3 or APN4. The total monosaccharide composition of APN (inclu-ding the N- linked glycans,...

Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

... 2559Structure of Anx(Gh1) in comparison with Anx24(Ca32)and AnxA5 The three-dimensional crystal structure of annexin G h1contains the typical annexin fold, well known from the studies of other members of ... raised the question of how this might be accomplished by plantannexins. As mentioned earlier, the endonexin sequence as aconstituent of canonical calcium binding sites in annexins isconserved in ... that the cotton annexin, in contrast to the bell pepper protein,provides canonical calcium binding sites in the ﬁrst domain. The observed conformation of the other domains does notallow binding...

Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies pptx

... predicted gen-eral feature of the mechanism is the devel-opment of a strong hydrogen bond between the incoming nucleophile and the leavinggroup in the transition state of the reaction. The phosphate ... recorded in the absence and presence of rescue reagent concentra-tions in the range 10–200 mm. Guanidine and propar-gylamine did not exhibit a signiﬁcant effect on the activity of the wild-type enzyme, ... hydrogen bonds from the NE and NH2 atoms of the guanidine sidechain of Arg535 to two oxygen atoms of phosphatedominate the contacts between the enzyme and the phosphate group [3,6]. The side chain...

Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt

... space-filling mode. The positions of the N- terminal and C-terminal amino acid residues of the model, the position of the flexibleloop region, which is omitted in the final model, and the position of the ... activation of KlPDCinvolves only a part of the processes in ScPDC,namely, binding of the regulatory substrate(s) in the cleft between the R and PP domains. The boundactivator may then enhance the ... Mg2+at the interface between twosubunits. The aminopyrimidine ring of ThDP is boundat the PYR domain of one subunit. The diphosphateresidue is bound to the PP domain of the other sub-unit at the...

Báo cáo khoa học: The oxygenase component of phenol hydroxylase from Acinetobacter radioresistens S13 docx

... resolution and a bandwidth of 2 nm. The concentration was 1.25 lMfor PHO. The spectrum wasanalyzed with the CDNNdeconvolution program [32].Construction and screening of the genomic library The ... structure has beenelucidated [19,20], allowed the identiﬁcation of the consen-sus sequences for the coordination of a dinuclear oxo-ironcentre. The presence of this kind of nonhaem iron is alsosuggested ... consumption of 14 ± 2 min)1, due to an uncoupled activity of PHR aloneupon phenolic substrates addition. The presence of PHR and PHO (in a reaction mixturecontaining NADH as electron donor) was not...

Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"

... finding' was anyold finding not expected by the attending physician.If an important finding (as mentioned in Table 1) was found,then we determined whether any action was taken because of the ... finding' was any oldfinding predicted by the attending physician; a 'new unex-pected finding' was any new finding not predicted by the attending physician; and an 'old unexpected ... revealed no differences betweenmedical and surgical patients with regard to the incidence of new and unexpected findings on daily routine CXRs and the effect of new and unexpected CXR findings on daily...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... [3].Interest in control mechanisms of cyclin B1 and cyclinB2 cell cycle-dependent transcription began early[3,30–33]. When investigating the regulation of humancyclin B1 transcription, a potential ... upregulation would indicate a function of Lin-9 inrepression. This indicates that Lin-9 is involved in the activation of these genes in cycling cells.In summary, it has been shown that the DREAMcomplex ... tothat of the CDE, with repression in resting cells andrelief from downregulation later in the cell cycle.Mutation of the CHR led to derepression of transcrip-tion in G0cells [10].Genes cannot...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: intersubunit interactions during virus assembly doc

... against the N- terminal end of helix 4 of the NTD of a neighboring subunit in the hexamer.Additional contacts involve helix 11 of the CTD and the C-terminal end of helix 7 of the NTD of the neigh-boring ... domains in eachhexamer, the dimerization interface between CTDdomains belonging to neighboring hexamers, andanother interface between CTD and NTD domainsbelonging to neighboring subunits of the ... [17,57]. In addition, processing of the MA-NTD linker allows the folding as a b-hair-pin of the NTD N- terminal segment, leading to a localconformational change in NTD. This rearrangementhas been proposed...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: designing inhibitors of capsid assembly ppt

... the assembly-competent mutants E187A and N1 83A: the conformations of the two assembly-incompetentmutants (Y169A and L211A) in the absence of CAIare the same as the structures of any mutant whencomplexed to the ... CTD-binding organic compound alsoestablishes hydrophobic contacts with regions involved in the interfacebetween the NTD and CTD. These results open new venues for the devel-opment of new antiviral ... peptide. Interestingly, Glu187(together with Ser178, Glu180 and Gln192) is one of the residues that decreases the association constant of CTD, as previously demonstrated in an alaninescanning of the...

Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

... 1A), and increas-ing concentrations of proBMP-2. A proBMP-2 concen-tration-dependent inhibition of the BMP-2-induced APactivity was observed (Fig. 1C). The possibility of con-tamination of the ... by noggin.In addition to the potential physiological implica-tions of our ﬁndings, the interaction of proBMP-2 withBMPR-IA and noggin allows indirect conclusionsabout the position of the ... immobilized noggin areindicated. The higher scattering of the sen-sorgrams in (C) and (D) is due to the factthat only 64 resonance units of noggin wereimmobilized in these experiments.Table 1. Kinetic...

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