... kept at 4 °C and used within 10 days.
Quantification of alkaline phosphatase and
aminopeptidase activities
Specific alkaline phosphatase (ALP) and N-aminopeptidase
(APN) enzymatic activities of BBMV ... Characterization of a Cry1Ac-receptor alkaline phosphatase
in susceptible and resistant
Heliothis virescens
larvae
Juan L. Jurat-Fuentes
1
and Micha...
... Miyamoto K, Tanaka K, Kawai M, Tainaka
K, Imada C, Okami Y & Inamori Y (1993) Cloning
and sequence of an alkaline serine protease-encoding
gene from the marine bacterium Alteromonas sp. strain
O-7. ... PCR was performed in 50 lL containing 1 ng
of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP
and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GA
AAAACCATGGTGAATGAATACCAAGCGACT-...
... with M. brassicae CSPMbraA6
[32]. We observed also that BrC15-Ac was able to displace
ASA, suggesting that brominated fatty acid and ASA both
associated with W81 in the same ligand binding site.
The ... purification of recombinant ASP3c.
(A) SDS/PAGE analysis of recombinant ASP3c secreted by Pichia
pastoris. Lane 1 shows standards (Low range and Polypeptide kits,
Bio-Rad, France) a...
... Tittmann
K (2005) Intermediates and transition states in thiamin
diphosphate-dependent decarboxylases. A kinetic and
NMR study on wild-type indolepyruvate decarboxylase
and variants using indolepyruvate, ... in vivo role in phenylalanine and tryptophan
catabolism [2]. It may also be involved in tyrosine
metabolism but, when alternative pathways are avail-
able, it is unlikel...
... amino acid sequence of hemerythrin from
Siphonosoma cumanense. Protein Seq Data Anal 3,
141–147.
43 Yano H, Satake K, Ueno Y & Tsugita A (1991) The
amino acid sequence of the beta chain of ... (http://www.fruitfly.org/seq_tools/
promoter.html) and has a probability of 0.98 (indicated by a red
arrow in A) . Enlarged (T) indicates possible transcription start. Putative
rib...
... The radioactivity was
counted on a gamma counter (Riastar, Packard Instrument,
USA), and each point was the mean of triplicates. Binding
data were analyzed using the RADLIG 4 software (BIO-
SOFT, ... genetic ability to thrive on pea seeds and
resist the toxic activity of pea albumin PA1b: five susceptible
strains ÔS. oryzae WAA42, Benin, and Bouriz, S. zeamais
LS, and S. granar...
... of trophic and toxic interactions
involving insects, comparative structural and func-
tional data on insect aminopeptidases are lacking.
In aphids, APN occurs in the apical network of
lamellae, ... by fitting data by a weighted linear regression using
the software SigmaPlotÒ. AlabNA,
L-alanine-b-naphthylamide; AlapNA, L-alanine-p-nitroanilide; ArgpNA, L-arginine-p-nitroanilide; M...
... 2387
Characterization of a novel long-chain acyl-CoA
thioesterase from Alcaligenes faecalis
Puja Shahi*
†
, Ish Kumar*
‡
, Ritu Sharma, Shefali Sanger and Ravinder S. Jolly
Institute of Microbial ... the
abnormal accumulation of intracellular acyl-CoA.
We have isolated a thioesterase from Alcaligenes
faecalis ISH108 and demonstrated its application in
chemoselective and ra...