Báo cáo khoa học: Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins doc
Báo cáo khoa học: Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins doc
... 495–522.Ó FEBS 2004 Dynamics of bacterial sHsps (Eur. J. Biochem. 271) 2503 Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins Nicolas Lentze1, J. Andrew Aquilina2, ... FEBS 2004elution of standard proteins was completely independent of temperature (Fig. 1D). Temperature- and concentration-dependentoligomerization of class B proteins A temperature- controlled ... defect of sHsps withFig. 8. Hypothetical model on chaperone dynamics and chaperone sub-strate interaction of small heat shock proteins (sHsps). For details, seethe text.Ó FEBS 2004 Dynamics of...
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Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot
... depletion of the 14-3-3f isoform sensitized to cell death, whereasoverexpression of this isoform delayed cell death. A combination of tandemaffinity purification and liquid chromatography–tandem ... C2-ceramide-induced apoptosisWith the aim of further analyzing the role of 14-3-3 proteins in apoptosis, an evaluation of the ability of proteins to bind and to be regulated by 14-3-3 proteins during C2-ceramide-induced ... activator of caspase (Smac), signal trans-ducer and activator of transcription 3 (STAT3), and hematopoietic progenitor kinase 1-interacting protein of 55 kDa (HIP-55). Using both MS and MALDI-TOF...
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Báo cáo khoa học: Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana doc
... volume of 5 mL each of a solution of 3-phenylpropionitrile (181.5 mg) and 3-phenylpropionicacid (205.2 mg) in methanol (10 mL), and Tris/HCl buffer(990 mL, 70 mM, pH 8.5) were mixed, and 5 ... determined from the plot of ratio areas vs. ratioconcentrations. One unit is de®ned as 1 lmol convert-edámin)1.Determination of temperature and pH optimum of AtNIT1 Temperature dependence. ... the enzyme stability of both crude extract and puri®ed enzyme: after 2 days atroom temperature and 3 months at 4 °C, 95% and 90%enzyme activity, respectively, remained. Temperature optimumThe...
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Tài liệu Báo cáo khoa học: Temperature and phosphate effects on allosteric phenomena of phosphofructokinase from a hibernating ground squirrel (Spermophilus lateralis) pptx
... inhibition of PFK may not bephysiologically relevant [14]. Temperature- dependentmechanisms of enzyme control may contribute to theregulation of PFK activity as the body temperature of hibernators ... skeletalmuscle of the golden-mantled ground squirrel (Spermo-philus lateralis). Particular attention is paid to theeffects of temperature on enzyme allostery and theinfluence of temperature in ... tempera-ture range of the plots were 103.2 ± 0.2 and 80.0 ± 1.2 kJ mol)1in the absence and presence of phosphate. For the 15–45 °C temperature range, theEa values were 25.7 ± 0.7 and 26.2 ± 1.1...
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Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt
... D esign and synthesis of a p otent c yclic ana-logue of the myelin basic p rotein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation forinduction of experimental ... side chain of Arg78 (Hg) and Glu82 (Hc1 )and the medium-range NOE between the side chains of Asp81(Hb) and Arg78 (Hg and Hc)]. In addition, Glu82 i nteractswiththesidechainofLys75.The ... chain of Arg78. The side chain of Arg78 is lesswell defined, as in the case of aqueous solution, because of the absence of interactions with the s ide chains of A sp81 (inthe agonist analogue) and...
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Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf
... composition and temperature influenceon neurolysin and TOP hydrolytic activities in order tounderstand their unusual variations of specificity [5,6] and broad spectrum of hydrolysis of biologically ... different temperatures and atdifferent salt concentrations [5]. Temperature dependence of the hydrolysis reactionrates of the substrates by neurolysisn and TOPThe temperature dependence of the ... to the temperature dependence of the catalytic constants accord-ing to the substrates, the positive and high values of DH* and DS* for hydrolysis are of note.Influence of the NaCl on TOP and neurolysin...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... studies of the structure and binding sites of enzymes. 8. Inhibitor binding toribonucleases. J Mol Biol 45, 491–511.33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea ... useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high affinityfor their target, along with small size and resistance to degradation. ... Department of Biology and Biochemistry, University of Bath, UK2 Department of Biochemistry, University of Wisconsin–Madison, USA3 Department of Chemistry, University of Wisconsin–Madison, USAIntroductionUpon...
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Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... structure of cN-IIshowing interfaces A and B and the Mg2+site. The inset showsthe tertiary structure of each subunit. Effector sites 1 and 2 and the active site are shown.Table 2. Effect of point ... inosine and IMP remains unaltered, and the effect of theenzyme activators ATP and Mg2+is also unchanged(Table 1). Furthermore, by simultaneously measuringthe rate of nucleoside and phosphate ... transfer of phosphate esterified in the 5¢ position of 6-hydroxypu-rine monophosphate nucleosides [1]. The transfer of phosphate can lead to phosphorylation of inosine,guanosine and a number of their...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filter paper assay and ... domain is mainly builtup by residues from the C-terminus and consists of atwo-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). Thetwo subunits ... base of bound GMP is stackedbetween Val143 and Phe194. N1, N2 and O6 of thenucleotide form hydrogen bonds to main chain atoms,whereas the side chain of Lys173 interacts with bothO6 and N7...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... performed usingT-COFFEE [44].Docking simulation of substrate on the active site of HYDJsThe substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were ... Homology model of HYDJs. (A) Docking of D-p-HPH to theHYDJsactive site. (B) The SGLs of HYDBpare shown as magentalines and those of HYDJsare shown in green. The transition state of D-p-HPH ... [39].Cloning and expression of putative HYDsThe genomic DNA of Jannaschia sp. CCS1 and Pseudomo-nas fluorescens PfO-1 were kindly supplied by Mary Moran(University of Georgia, Athens, GA) and Stuart...
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